关键词: 崂山奶山羊, 微卫星, 泌乳量, 乳脂率, 乳蛋白率

Abstract:

【Objective】 The aim of the present work was to research whether the microsatellite DNA could improve milk performance traits of Laoshan dairy goat as the genetic marker. 【Method】 According to comparative genetic map, five dairy cattle microsatellite loci (CSSM66, BM302, BM1329, BM143 and BMS0812) correlated with milk performance traits of chromosome 4, 6 and 15 were detected in 175 Laoshan dairy goats. The genetic analysis and correlation analysis between microsatellite markers and milk performance traits were calculated in Laoshan dairy goat. 【Result】 The genetic analysis showed that 5 microsatellite loci had a rich diversity in Laoshan dairy goat group. The mean values of the Ne, Ho, PIC and Fis were 0.39924, 0.4667, 0.6748 and 0.347 respectively. Least square analysis evidenced that CSSM66 had a significant effect on the fat percentage (P＜0.01) and 300 d milk yield (P＜0.05), and the genotypes 210/210 and 194/194 were the most favorable genotypes respectively, the alleles 210 and 194 had a positive effect on fat percentage and 300 d milk yield respectively. BM302 had a significant effect on 300 d milk yield(P＜0.01), and the genotype 156/150 was the most favorable genotype, the alleles 156 and 150 had a positive effect on 300 d milk yield. BM143 had a significant effect on the fat percentage (P＜0.05) and protein percentage (P＜0.01), and the genotypes 114/100 and 100/100 were the most favorable genotypes, both the alleles 114 and 100 had a positive effect on fat percentage and protein percentage. BM1329 had a significant effect on fat percentage (P＜0.05), and the genotype 182/180 was the most favorable genotype. 【Conclusion】 In this study, the microsatellite loci with a rich diversity and a significant effect on milk performance traits were found, and they provided valuable genetic markers for marker-assisted selection of Laoshan dairy goat.

Key words: laoshan dairy goat, microsatellite, milk yield, fat percentage, protein percentage