关键词: 鸡F2资源群, 冠型性状, 微卫星标记, 连锁图谱, 连锁分析

Abstract:

【Objective】 To establish a linkage map of the chromosome 2 in the chicken-F2 resource group with cockscomb trait record, and to determine a preliminary mapping for the gene of controlling comb trait combined with the phenotypic analysis.【Method】 Through test cross, 2 Tulufan fighting cocks with the homozygous pea-comb genotype and 6 rose comb hens with the homozygous rose-comb genotype were chosen as the parents. Four hundred and thirty six chickens of the F2 generation group were built by the F2 generation experimental design, and the genetic linkage map of EL (East Lansing) family was also referred. Meanwhile, the 14 microsatellite markers on the chromosome 2 of chicken were selected, and the family genetic linkage map of chromosome 2 was built preliminarily by using MapMaker/EXP3.0 software. In addition, the linkage analysis of cocks comb gene was completed through the LOD scoring method. 【Result】 By the χ2 test, the proportion of differentiation of the F2 resource group was accorded with the law of independent segregation of classic Mendelian inheritance. Except for the MCW0157, the other 13 microsatellite markers all could have linkage, and the genetic linkage map of chromosome 2 in this family was similar with the EL family’s. 【Conclusion】 The site MCW0082 of chicken chromosome 2 may have linkage with the rose comb gene.

Key words: chicken-F2 resource group, comb trait, microsatellite marker, linkage map, linkage analysis