氯氟氰菊酯对斜纹夜蛾抗性和敏感种群表皮穿透比较

doi:10.3864/j.issn.0578-1752.2009.07.017

中国农业科学 ›› 2009, Vol. 42 ›› Issue (7): 2386-2391 .doi: 10.3864/j.issn.0578-1752.2009.07.017

氯氟氰菊酯对斜纹夜蛾抗性和敏感种群表皮穿透比较

刘永杰,沈晋良,杨田堂,肖鹏,贺金#br#

（山东农业大学植物保护学院）

收稿日期:2008-11-11 修回日期:2009-02-10 出版日期:2009-07-10 发布日期:2009-07-10

Comparison of Cuticular Penetration Between Susceptible and Lambda-Cyhalothrin-Resistant Populations in Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae)#br#

LIU Yong-jie, SHEN Jin-liang, YANG Tian-tang, XIAO Peng, HE Jin#br#

（山东农业大学植物保护学院）

Received:2008-11-11 Revised:2009-02-10 Online:2009-07-10 Published:2009-07-10

摘要/Abstract

摘要：

【目的】研究证实斜纹夜蛾对氯氟氰菊酯的抗性是否与其表皮穿透率降低有关。【方法】分别采用点滴法、浸叶法和叶片夹毒法测定了斜纹夜蛾（Spodoptera litura Fabricius）对氯氟氰菊酯的抗性，用14C标记氯氟氰菊酯测定了斜纹夜蛾抗性和敏感种群3龄和5龄幼虫的表皮穿透率。【结果】采用3种方法测定结果表明，3龄幼虫抗性水平的次序为点滴法（抗性倍数为2 765.4倍）＞浸叶法（365.1倍）＞叶片夹毒法（34.0倍），点滴法、浸叶法及叶片夹毒法间抗性倍数之比依次为81.3﹕10.7﹕1;5龄幼虫抗性水平的次序为点滴法（抗性倍数为2 271.3倍）＞浸叶法（257.2倍）＞叶片夹毒法（20.2倍），点滴法、浸叶法及叶片夹毒法间抗性倍数之比依次为112.4﹕12.7﹕1。经穿透昆虫表皮的触杀毒力所引起的抗性高于经口器和表皮进入的胃毒毒力和触杀毒力之和所引起的抗性，更明显高于经口器进入的胃毒毒力所引起的抗性。同位素标记氯氟氰菊酯测定结果表明，处理后0.5 h和1.0 h，抗性种群和敏感种群间表皮穿透率差异不显著，处理后2 h，两种群间的穿透率存在明显差异，至处理后8 h，敏感种群3龄和5龄幼虫的表皮穿透率分别为46.3%和43.3%，抗性种群3龄和5龄幼虫的表皮穿透率分别为35.7%和29.1%，氯氟氰菊酯对抗性种群3龄幼虫的表皮穿透率仅为敏感种群幼虫穿透率的0.77倍，对5龄幼虫的表皮穿透率仅为敏感种群幼虫穿透率的0.67倍，抗性种群的穿透率明显慢于敏感种群的穿透率。【结论】斜纹夜蛾抗性种群对氯氟氰菊酯的表皮穿透率明显低于敏感种群，可能是其产生抗药性的一个原因。

关键词: 甜菜夜蛾')">甜菜夜蛾, 抗药性, 氯氟氰菊酯, 表皮穿透率

Abstract:

【Objective】 In this paper, the relationship of the resistance of the common cutworm, Spodoptera litura Fabricius, to lambda-cyhalothrin and cuticular penetration of lambda-cyhalothrin was studied. 【Method】 Topical application, leaf-dipping and leaf-sandwich methods were used to determine the resistance levels of Spodoptera litura to lambda-cyhalothrin and cuticular penetration of 14C-lambda-cyhalothrin was carried out. 【Result】 The results indicate that the sequence of resistance levels of the 3rd instar larvae was topical application (2765.4 fold) ＞leaf-dipping method (365.1 fold) ＞leaf-sandwich method (34.0 fold) and the sequence of resistance levels of the 5th instar larvae was also topical application (2271.3 fold) ＞leaf-dipping method (257.2 fold) ＞leaf-sandwich method (20.2 fold). The resistance levels of the 3rd instar larvae were 81.3 and 10.7 times higher obtained by topical application (contact toxicity) and leaf-dipping method (contact toxicity and stomach toxicity) than that by leaf-sandwich method (stomach toxicity). Compared in the same way, the resistance levels of the 5th instar larvae were 112.4 and 12.7 times higher. Therefore, the resistant population of Spodoptera litura has a resistance level obtained by way of contact toxicity at least 81.3 times than that of stomach toxicity. After 0.5 h and 1.0 h treatments, the results indicate that there was no significant difference of cuticular penetration of 14C-lambda-cyhalothrin between the resistant and susceptible populations. After 2 h treatment, the rate of cuticular penetration of the resistant population became a little slower than that of the susceptible population. After 8h treatment, the rate of cuticular penetration in 3rd and 5th instar larvae of the resistant population was about 0.77 and 0.67 folds of that in the susceptible population. 【Conclusion】 Delayed cuticular penetration is responsible for the resistance of Spodoptera litura to lambda-cyhalothrin.

Key words: Spodoptera litura')">Spodoptera litura, insecticide resistance, lambda-cyhalothrin, cuticular penetration

刘永杰,沈晋良,杨田堂,肖鹏,贺金
. 氯氟氰菊酯对斜纹夜蛾抗性和敏感种群表皮穿透比较[J]. 中国农业科学, 2009, 42(7): 2386-2391 .

LIU Yong-jie,SHEN Jin-liang,YANG Tian-tang,XIAO Peng,HE Jin
. Comparison of Cuticular Penetration Between Susceptible and Lambda-Cyhalothrin-Resistant Populations in Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae)#br# [J]. Scientia Agricultura Sinica, 2009, 42(7): 2386-2391 .

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氯氟氰菊酯对斜纹夜蛾抗性和敏感种群表皮穿透比较

Comparison of Cuticular Penetration Between Susceptible and Lambda-Cyhalothrin-Resistant Populations in Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae)#br#

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