关键词: 2-十二烷基环丁酮, 辐照, 硅胶柱, 气相色谱-质谱联用

Abstract:

【Objective】 A novel method was developed for quantitative determination of 2-dodecylcyclobutanone which is one of the specific markers of irradiated fat containing foods. 【Method】 The method includes extraction and separation by silica gel column and analysis by capillary column gas chromatography-mass spectrometry (GC-MS). And the correlative parameters of the concentration of eluting solvent, extent of deactivated silica, and collecting time for separation and chromatogram detection were optimized. 【Result】The limit of detection was 0.005 mg?L-1, the recoveries were 75.9%-103.9% according to adding concentration of 0.1-2.0 mg?L-1, and the relative standard deviations were all below 5.7%. 【Conclusion】It showed that the silica gel column separation method has lower cost, higher separative efficiency, better stability, lower detection limit, and larger analytic sample as compared to the EN1785 method.

Key words: 2-dodecylcyclobutanone, irradiation, silica column, gas chromatography-mass spectrometry