中国农业科学 ›› 2008, Vol. 41 ›› Issue (5): 1341-1346 .doi: 10.3864/j.issn.0578-1752.2008.05.011

• 植物保护 • 上一篇    下一篇

DNA微阵列技术在病原细菌基因转录谱分析中的应用

高世强,张新建,吴茂森,何晨阳   

  1. 中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室
  • 收稿日期:2007-03-15 修回日期:1900-01-01 出版日期:2008-05-10 发布日期:2008-05-10
  • 通讯作者: 何晨阳

Transcriptional profile of plant pathogenic bacteria revealed by DNA microarray analysis

Shi-Qiangg GAO Chenyang He   

  1. 中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室
  • Received:2007-03-15 Revised:1900-01-01 Online:2008-05-10 Published:2008-05-10
  • Contact: Chenyang He

摘要: DNA微阵列技术是近年来发展起来的一种功能基因组学研究技术。随着这项新技术的建立和日趋完善及其在植物病理学研究中的应用,为病原细菌致病机理的研究带来了新思路并开辟了新途径。利用DNA微阵列技术,检测病原细菌在不同生长条件下和在侵染植物过程中的基因转录谱,可以从基因组水平上鉴定出新的与毒性和适应性相关的基因及其表达调控网络,有助于阐明这些基因的生物学功能及其机理。本文结合本实验室近几年来的相关研究结果,综述DNA微阵列技术在病原细菌转录谱研究中应用的最新进展。

关键词: DNA微阵列, 植物病原细菌, 基因表达谱, 病原物—寄主互作, 致病机制

Abstract: With the completion of full genomic sequences of many bacterial phytopathogens, the development of microarray profiling technology provides new insights into the study of pathogen-host interactions. Using DNA microarray technology to detect the transcriptional profiles of bacterial pathogens under different conditions (especially during in vivo infection) could identify pathogenicity-related genes and their regulatory networks. These would gain helpful information to understand the gene function and pathogenic mechanisms of pathogens. Microarrays therefore provide new ideas for the prevention of plant pathogenic bacteria. Here we will review the latest application of DNA microarray technology in the transcriptional profile study of plant pathogenic bacteria.

Key words: DNA microarray, plant pathogenic bacteria, gene expression profiling, pathogen-host interactions, pathogenic mechanisms