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Elucidation of the structure, antioxidant, and interfacial properties of flaxseed proteins tailored by microwave treatment
YU Xiao, DUAN Zi-qiang, QIN Xiao-peng, ZHU Ying-ying, HUANG Feng-hong, PENG Deng-feng, BAI Yan-hong, DENG Qian-chun
2023, 22 (5): 1574-1589.   DOI: 10.1016/j.jia.2023.04.021
Abstract306)      PDF in ScienceDirect      
The microwave treatment is commonly applied to flaxseed to release nutrients, inactivate enzymes, remove cyanogens, and intensify flavors. The current study aimed to explore the influences of microwave exposure on the antioxidant and interfacial properties of flaxseed protein isolates (FPI), focusing on the altering composition and molecular structure. The results showed that after microwave exposure (700 W, 1–5 min), more compact assembly of storage proteins and subsequent permeation by membrane fragments of oil bodies occurred for cold-pressing flaxseed flours. Moreover, the particle sizes of FPI was progressively reduced with the decrement ranged from 37.84 to 60.66% , whereas the zeta potential values initially decreased and then substantially recovered during 1–5 min of microwave exposure. The conformation unfolding, chain cross-linking, and depolymerization were sequentially induced for FPI based on the analysis of fluorescence emission spectra, secondary structure, and protein subunit profiles, thereby affecting the dispersion or aggregation properties between albumin and globulin fractions in FPI. Microwave exposure retained specific phenolic acids and superior antioxidant activities of FPI. The inferior gas–water interface absorption and the loose/porous assembly structure were observed for the foams prepared by FPI, concurrent with obviously shrinking foaming properties upon microwave exposure. Improving oil–water interface activities of FPI produced the emulsion droplets with descending sizes and dense interface coating, which were then mildly destabilized due to the lipid leakage and weakened rheological behavior with microwave exposure extended to 5 min. Our findings elucidated that microwave treatment could tailor the application functionality of protein fractions in flaxseed based on their structural remodeling.
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Transcriptomic profiling of watermelon (Citrullus lanatus) provides insights into male flowers development
ZHU Ying-chun, YUAN Gao-peng, JIA Sheng-feng, AN Guo-lin, LI Wei-hua, SUN De-xi, LIU Jun-pu
2022, 21 (2): 407-421.   DOI: 10.1016/S2095-3119(21)63615-8
Abstract265)      PDF in ScienceDirect      
Watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai) is an important cucurbit crop grown worldwide.  Watermelon fruit quality, fertility, and seed-setting rate are closely related to male flower development.  In this study, the different developmental stages of flower buds of the watermelon cultivar ‘Xinteda Zhengkang 9’ were distinguished by cytological observation, and transcriptome sequencing analysis was performed subsequently.  Acetocarmine staining of anthers was performed and the longitudinal and transverse diameters of the unopened male flower buds were measured.  Cytological observations of anthers at different developmental stages showed that the anther grew from the tetrad to the mature stage, and the longitudinal and transverse diameters of the flower buds increased.  The length of the male flower buds also changed significantly during development.  Transcriptome sequencing analysis at four periods, the tetrad (A group), mononuclear (B group), dikaryophase (C group), and mature stages (D group).  A total of 16 288 differentially expressed genes (DEGs) were detected in the four stages, with the prolongation of developmental stages, the number of DEGs increased gradually in the comparison groups, there was 2 014, 3 259, 4 628, 1 490, 3 495 and 1 132 DEGs revealed in six comparison groups (A-vs.-B, A-vs.-C, A-vs-D, B-vs.-C, B-vs.-D, and C-vs.-D), respectively.  Gene Ontology (GO) and KEGG enrichment analysis showed that the DEGs were mainly enriched in cellular component and starch and sucrose metabolism, phenylpropanoid biosynthesis and pentose sugar, etc.  Finally, we completely screened 59 DEGs in the six comparison groups, interestingly, we found one pollen-specific protein (Cla001608) that was significantly down-regulated (the value of log2Fold Change up to 17.32), which indicated that it may play an important role in the development of male flowers.  This work provides insight into the molecular basis of the developmental stages of male flowers in watermelon and may aid in dominant cross breeding.

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Yield performance and optimal nitrogen and phosphorus application rates in wheat and faba bean intercropping
XIAO Jing-xiu, ZHU Ying-an, BAI Wen-lian, LIU Zhen-yang, TANG Li, ZHENG Yi
2021, 20 (11): 3012-3025.   DOI: 10.1016/S2095-3119(20)63489-X
Abstract188)      PDF in ScienceDirect      
Yield performance in cereal and legume intercropping is related to nutrient management, however, the yield response of companion crops to nitrogen (N) input is inconclusive and only limited efforts have focused on rationed phosphorous (P) fertilization.  In this study, two multi-year field experiments were implemented from 2014–2019 under identical conditions.  Two factors in a randomized complete block design were adopted in both experiments.  In field experiment 1, the two factors included three planting patterns (mono-cropped wheat (MW), mono-cropped faba bean (MF), and wheat and faba bean intercropping (W//F)) and four N application rates (N0, 0 kg N ha–1; N1, 90 and 45 kg N ha–1 for wheat and faba beans, respectively; N2, 180 and 90 kg N ha–1 for wheat and faba beans, respectively; and N3, 270 and 135 kg N ha–1 for wheat and faba beans, respectively).  In field experiment 2, the two factors included three P application rates (P0, 0 kg P2O5 ha–1; P1, 45 kg P2O5 ha–1; and P2, 90 kg P2O5 ha–1) and the same three planting patterns (MW, MF, and W//F).  The yield performances of inter- and mono-cropped wheat and faba beans under different N and P application rates were analyzed and the optimal N and P rates for intercropped wheat (IW) and MW were estimated.  The results revealed that intercropping favored wheat yield and was adverse to faba bean yield.  Wheat yield increased by 18–26%, but faba bean yield decreased by 5–21% in W//F compared to MW and MF, respectively.  The stimulated IW yield drove the yield advantage in W//F with an average land equivalent ratio (LER) of 1.12.  N and P fertilization benefited IW yield, but reduced intercropped faba bean (IF) yield.  Nevertheless, the partial LER of wheat (pLERwheat) decreased with increasing N application rates, and the partial LER of faba bean (pLERfaba bean) decreased with increasing P application rates.  Thus, LER decreased as N input increased and tended to decline as P rates increased.  IW maintained a similar yield as MW, even under reduced 40–50% N fertilizer and 30–40% P fertilizer conditions.  The estimated optimum N application rates for IW and MW were 150 and 168 kg ha–1, respectively, and 63 and 62 kg ha–1 for P2O5, respectively.  In conclusion, W//F exhibited yield advantages due to stimulated IW yield, but the intercropping yield benefit decreased as N and P inputs increased.  Thus, it was concluded that modulated N and P rates could maximize the economic and ecological functions of intercropping.  Based on the results, rates of 150 kg N ha–1 and 60 kg P2O5 ha–1 are recommended for IW production in southwestern China and places with similar conditions.
 
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Comparative transcriptome analysis of the effect of different heat shock periods on the unfertilized ovule in watermelon (Citrullus lanatus)
ZHU Ying-chun, SUN De-xi, DENG Yun, AN Guo-lin, LI Wei-hua, SI Wen-jing, LIU Jun-pu, SUN Xiao-wu
2020, 19 (2): 528-540.   DOI: 10.1016/S2095-3119(19)62777-2
Abstract111)      PDF in ScienceDirect      

In vitro gynogenesis is an important tool used in haploid or homozygous double-haploid plant breeding.  However, because of low repeatability, embryoid induction rate and quality, the molecular mechanisms remain poorly understood.  Heat shock treatment can promote the transformation of the gametophytic pathway into the sporophyte pathway, which induces the occurrence of haploid.  In this study, unfertilized ovaries were heat shocked for 0 h (A0) before flowering and for 0 h (A1), 4 h (A3), 8 h (A5), 12 h (A7), and 24 h (A8), respectively, at 37°C at the first day of the flowering stage.  The ovule enlargement rate was increased from 0% at 25°C to 96.8% at 37°C (24 h treatment).  Thus, we aimed to investigate the gene expression patterns in unfertilized ovules of watermelon after different periods of heat shock by using RNA-Seq technology.  The results showed that compared with A3, A5, A7, and A8, the biosynthesis of amino acid, glycine, serine and threonine metabolic pathways in A1 has changed significantly.  This indicated that heat shock treatment affected the synthesis and transformation of amino acids during ovule expansion.  The transcriptome data suggested gene expressions of ovule growth were significantly changed by heat-specific influences.  The results provide new information on the complex relationship between in vitro gynogenesis and temperature.  This provides a basis for further study of the mechanism of heat shock affecting the expansion of watermelon ovule. 

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Yield characteristics of japonica/indica hybrids rice in the middle and lower reaches of the Yangtze River in China
XU Dong, ZHU Ying, CHEN Zhi-feng, HAN Chao, HU Lei, QIU Shi, WU Pei, LIU Guo-dong, WEI Hai-yan, ZHANG Hong-cheng
2020, 19 (10): 2394-2406.   DOI: 10.1016/S2095-3119(19)62872-8
Abstract103)      PDF in ScienceDirect      
Although a lot of researches have been done on yield characteristics of japonica/indica hybrid rice, there is little information on differences of yield characteristics between different types of hybrid.  To determine common characteristics of japonica/indica hybrid rice (JIHR) and identify the differences between different types of JIHR, the present study assessed yield characteristics, such as panicle trait, leaf area index (LAI), above-ground biomass accumulation, and nitrogen absorption and utilization, among three types of cultivar of JIHR.  In our field experiments, three types of JIHR, e.g., Yongyou, Chunyou and Jiayouzhongke, were divided, and each of them has two cultivars, which were used as materials, meanwhile, using conventional japonica rice (CJR) Wuyingjing 31 and Sujing 9 were as controls.  The results showed that the mean yield of those JIHR was above 12 t ha–1 in 2017 and 2018, and was 31.9 and 32.2%, respectively higher than that of CJR in the two years.  Spikelet number per panicle of JIHR resulted in high yield.  Higher yield of JIHR was likely contributed to greater panicle number and more spikelets per panicle.  Higher yielding JIHR showed stronger tillering capacity, larger LAI and above-ground biomass accumulation from jointing to heading stages, which likely contributed to the higher number of spikelets per panicle.  The long duration from heading to maturity stages allowed more nitrogen accumulation of higher yielding JIHR.
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Combined effect of shading time and nitrogen level on grain filling and grain quality in japonica super rice
WEI Hai-yan, ZHU Ying, QIU Shi, HAN Chao, HU Lei, XU Dong, ZHOU Nian-bing, XING Zhi-peng, HU Ya-jie, CUI Pei-yuan, DAI Qi-gen, ZHANG Hong-cheng
2018, 17 (11): 2405-2417.   DOI: 10.1016/S2095-3119(18)62025-8
Abstract369)      PDF (1180KB)(727)      
There is limited information about the combined effect of shading time and nitrogen (N) on grain filling and quality of rice.  Therefore, two japonica super rice cultivars, Nanjing 44 and Ningjing 3, were used to study the effect of shading time and N level on the characteristics of rice panicle and grain filling as well as the corresponding yield and quality.  At a low N level (150 kg N ha–1, 150N), grain yield decreased (by 21.07–26.07%) under the treatment of 20 days of shading before heading (BH) compared with the no shading (NS) treatment.  These decreases occurred because of shortened panicle length, decreased number of primary and secondary branches, as well as the grain number and weight per panicle.  At 150N, in the treatment of 20 days of shading after heading (AH), grain yield also decreased (by 9.46–10.60%) due to the lower grain weight per panicle.  The interaction of shading and N level had a significant effect on the number of primary and secondary branches.  A high level of N (300 kg N ha–1, 300N) could offset the negative effect of shading on the number of secondary branches and grain weight per panicle, and consequently increased the grain yield in both shading treatments.  In superior grains, compared with 150N NS, the time to reach 99% of the grain weight (T99) was shortened by 1.6 to 1.7 days, and the grain weight was decreased by 4.18–5.91% in 150N BH.  In 150N AH, the grain weight was 13.39–13.92% lower than that in 150N NS due to the slow mean and the maximum grain-filling rate (GRmean and GRmax).  In inferior grains, grain weight and GRmean had a tendency of 150N NS>150N BH>150N AH.  Under shaded conditions, 300N decreased the grain weight due to lower GRmean both in superior and inferior grains.  Compared with 150N NS, the milling and appearance qualities as well as eating and cooking quality were all decreased in 150N BH and 150N AH.  Shading with the high level of 300N improved the milling quality and decreased the number of chalky rice kernels, but the eating and cooking quality was reduced with increased chalky area and overall chalkiness.  Therefore, in the case of short term shading, appropriate N fertilizer could be used to improve the yield and milling quality of rice, but limited application of N fertilizer is recommended to achieve good eating and cooking quality of rice.
 
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