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Improved multi-scale inverse bottleneck residual network based on triplet parallel attention for apple leaf disease identification

Lei Tang, Jizheng Yi, Xiaoyao Li
2024, 23 (3): 901-922.   DOI: 10.1016/j.jia.2023.06.023
Abstract138)      PDF in ScienceDirect      
Accurate diagnosis of apple leaf diseases is crucial for improving the quality of apple production and promoting the development of the apple industry.  However, apple leaf diseases do not differ significantly from image texture and structural information.  The difficulties in disease feature extraction in complex backgrounds slow the related research progress.  To address the problems, this paper proposes an improved multi-scale inverse bottleneck residual network model based on a triplet parallel attention mechanism, which is built upon ResNet-50, while improving and combining the inception module and ResNext inverse bottleneck blocks, to recognize seven types of apple leaf (including six diseases of alternaria leaf spot, brown spot, grey spot, mosaic, rust, scab, and one healthy).  First, the 3×3 convolutions in some of the residual modules are replaced by multi-scale residual convolutions, the convolution kernels of different sizes contained in each branch of the multi-scale convolution are applied to extract feature maps of different sizes, and the outputs of these branches are multi-scale fused by summing to enrich the output features of the images.  Second, the global layer-wise dynamic coordinated inverse bottleneck structure is used to reduce the network feature loss.  The inverse bottleneck structure makes the image information less lossy when transforming from different dimensional feature spaces.  The fusion of multi-scale and layer-wise dynamic coordinated inverse bottlenecks makes the model effectively balances computational efficiency and feature representation capability, and more robust with a combination of horizontal and vertical features in the fine identification of apple leaf diseases.  Finally, after each improved module, a triplet parallel attention module is integrated with cross-dimensional interactions among channels through rotations and residual transformations, which improves the parallel search efficiency of important features and the recognition rate of the network with relatively small computational costs while the dimensional dependencies are improved.  To verify the validity of the model in this paper, we uniformly enhance apple leaf disease images screened from the public data sets of Plant Village, Baidu Flying Paddle, and the Internet.  The final processed image count is 14,000.  The ablation study, pre-processing comparison, and method comparison are conducted on the processed datasets.  The experimental results demonstrate that the proposed method reaches 98.73% accuracy on the adopted datasets, which is 1.82% higher than the classical ResNet-50 model, and 0.29% better than the apple leaf disease datasets before preprocessing.  It also achieves competitive results in apple leaf disease identification compared to some state-of-the-art methods.
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Yield performance and optimal nitrogen and phosphorus application rates in wheat and faba bean intercropping
XIAO Jing-xiu, ZHU Ying-an, BAI Wen-lian, LIU Zhen-yang, TANG Li, ZHENG Yi
2021, 20 (11): 3012-3025.   DOI: 10.1016/S2095-3119(20)63489-X
Abstract188)      PDF in ScienceDirect      
Yield performance in cereal and legume intercropping is related to nutrient management, however, the yield response of companion crops to nitrogen (N) input is inconclusive and only limited efforts have focused on rationed phosphorous (P) fertilization.  In this study, two multi-year field experiments were implemented from 2014–2019 under identical conditions.  Two factors in a randomized complete block design were adopted in both experiments.  In field experiment 1, the two factors included three planting patterns (mono-cropped wheat (MW), mono-cropped faba bean (MF), and wheat and faba bean intercropping (W//F)) and four N application rates (N0, 0 kg N ha–1; N1, 90 and 45 kg N ha–1 for wheat and faba beans, respectively; N2, 180 and 90 kg N ha–1 for wheat and faba beans, respectively; and N3, 270 and 135 kg N ha–1 for wheat and faba beans, respectively).  In field experiment 2, the two factors included three P application rates (P0, 0 kg P2O5 ha–1; P1, 45 kg P2O5 ha–1; and P2, 90 kg P2O5 ha–1) and the same three planting patterns (MW, MF, and W//F).  The yield performances of inter- and mono-cropped wheat and faba beans under different N and P application rates were analyzed and the optimal N and P rates for intercropped wheat (IW) and MW were estimated.  The results revealed that intercropping favored wheat yield and was adverse to faba bean yield.  Wheat yield increased by 18–26%, but faba bean yield decreased by 5–21% in W//F compared to MW and MF, respectively.  The stimulated IW yield drove the yield advantage in W//F with an average land equivalent ratio (LER) of 1.12.  N and P fertilization benefited IW yield, but reduced intercropped faba bean (IF) yield.  Nevertheless, the partial LER of wheat (pLERwheat) decreased with increasing N application rates, and the partial LER of faba bean (pLERfaba bean) decreased with increasing P application rates.  Thus, LER decreased as N input increased and tended to decline as P rates increased.  IW maintained a similar yield as MW, even under reduced 40–50% N fertilizer and 30–40% P fertilizer conditions.  The estimated optimum N application rates for IW and MW were 150 and 168 kg ha–1, respectively, and 63 and 62 kg ha–1 for P2O5, respectively.  In conclusion, W//F exhibited yield advantages due to stimulated IW yield, but the intercropping yield benefit decreased as N and P inputs increased.  Thus, it was concluded that modulated N and P rates could maximize the economic and ecological functions of intercropping.  Based on the results, rates of 150 kg N ha–1 and 60 kg P2O5 ha–1 are recommended for IW production in southwestern China and places with similar conditions.
 
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Supplemental blue light increases growth and quality of greenhouse pak choi depending on cultivar and supplemental light intensity
ZHENG Yin-jian, ZHANG Yi-ting, LIU Hou-cheng, LI Ya-min, LIU Ying-liang, HAO Yan-wei, LEI Bing-fu
2018, 17 (10): 2245-2256.   DOI: 10.1016/S2095-3119(18)62064-7
Abstract364)      PDF in ScienceDirect      
To evaluate the supplementary blue light intensity on growth and health-promoting compounds in pak choi (Brassica campestris ssp. chinensis var. communis), four blue light intensity treatments (T0, T50, T100 and T150 indicate 0, 50, 100, and 150 μmol m–2 s–1, respectively) were applied 10 days before harvest under greenhouse conditions.  Both of cultivars (green- and red-leaf pak choi) under T50 had the highest yield, content of chlorophyll and sugars.  With light intensity increasing, antioxidant compounds (vitamin C and carotenoids) significantly increased, while nitrate content showed an opposite trend.  The health-promoting compounds (phenolics, flavonoids, anthocyanins, and glucosinolates) were significantly higher under supplementary light treatment than T0, so as the antioxidant capacity (2,2-diphenyl-1-picrylhydrazyl and ferric-reducing antioxidant power).  The species-specific differences in photosynthetic pigment and health-promoting compounds was found in green- and red-leaf pak choi.  T50 treatment could be used for yield improvement, whereas T100 treatment could be applied for quality improvement.  Results showed that blue light intensity can regulate the accumulation of biomass, morphology and health-promoting compounds in pak choi under greenhouse conditions.
 
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Over-Expression of ScMnSOD, a SOD Gene Derived from Jojoba, Improve Drought Tolerance in Arabidopsis
LIU Xiao-fei, SUN Wei-min, LI Ze-qin, BAI Rui-xue, LI Jing-xiao, SHI Zi-han, GENG Hongwei, ZHENG Ying, ZHANG Jun , ZHANG Gen-fa
2013, 12 (10): 1722-1730.   DOI: 10.1016/S2095-3119(13)60404-9
Abstract1278)      PDF in ScienceDirect      
Jojoba (Simmondsia chinensis) is mainly distributed in desert, and the molecular mechanisms of jojoba in response to abiotic stress still remain elusive. In this paper, we cloned and characterized a SOD gene from jojoba named as ScMnSOD, and introduced into Arabidopsis to investigate its functions of responding to drought stress. The transgenic Arabidopsis showed an improvement in drought tolerance. Moreover, under a water deficit condition, the accumulation of reactive oxygen species (ROS) was remarkably decreased in the transgenic lines compared to the WT. Furthermore, the ScMnSOD promoter was cloned to the 5´-upstream of GUS coding region in a binary vector, and introduced into Arabidopsis. And results showed that ScMnSOD expression can be induced by drought, salt, ABA, and low temperature. In conclusion, ScMnSOD plays an important role in drought tolerance which is, at least partially, attributed to its role in ROS detoxification.
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