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Journal of Integrative Agriculture
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First report of the South American tomato leafminer,
Tuta absoluta
(Meyrick), in China
ZHANG Gui-fen, MA De-ying, WANG Yu-sheng, GAO You-hua, LIU Wan-xue, ZHANG Rong, FU Wen-jun, XIAN Xiao-qing, WANG Jun, KUANG Meng, WAN Fang-hao
2020, 19 (
7
): 1912-1917. DOI:
10.1016/S2095-3119(20)63165-3
Abstract
(
497
)
PDF in ScienceDirect
Tuta absoluta
(Meyrick) originated in South America and is one of the most serious pests of tomatoes. It is also known to attack other solanaceous crops, including potato, eggplant, pepper, tobacco, and weedy species such as black nightshade. After accidental introduction into Spain in 2006, this pest spread rapidly throughout Afro-Eurasia and has become a major threat to tomato production worldwide. Here, we report the first record of
T. absoluta
as an invasive pest in China. It was found in tomato fields in Ili Kazakg Autonomous Prefecture, Xinjiang Uygur Autonomous Region (Ili, Xinjiang), China, and its occurrence was confirmed by both morphological and molecular approaches. In Ili,
T. absoluta
has been found to attack eggplant, potato, and black nightshade. We found the larvae generally mining and feeding on leaves and boring into tomato fruits, with multiple larvae sometimes observed in a single fruit. Its infestation levels differ among the tomato fields and host species. In all of the surveyed tomato fields,
T. absoluta
infested 100% of plants. In some of the fields, up to 90% of the eggplant and 100% of the potato plants were infested. Since no natural enemies were found under field conditions, suitable management practices are urgently needed to stop the further spread of this destructive pest in China.
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Heterologous expression of the
ThIPK2
gene enhances drought resistance of common wheat
ZHANG Shu-juan, LI Yu-lian, SONG Guo-qi, GAO Jie, ZHANG Rong-zhi, LI Wei, CHEN Ming-li, LI Gen-ying
2020, 19 (
4
): 941-952. DOI:
10.1016/S2095-3119(19)62714-0
Abstract
(
129
)
PDF in ScienceDirect
ThIPK2
is an inositol polyphosphate kinase gene cloned from Thellungiella halophila that participates in diverse cellular processes. Drought is a major limiting factor in wheat (
Triticum aestivum
L.) production. The present study investigated whether the application of the
ThIPK2
gene could increase the drought resistance of transgenic wheat. The codon-optimized
ThIPK2
gene was transferred into common wheat through
Agrobacterium
-mediated transformation driven by either a constitutive maize ubiquitin promoter or a stress-inducible rd29A promoter from Arabidopsis. Molecular characterization confirmed the presence of the foreign gene in the transformed plants. The transgenic expression of
ThIPK2
in wheat led to significantly improve drought tolerance compared to that observed in control plants. Compared to the wild type (WT) plants, the transgenic plants showed higher seed germination rates, better developed root systems, a higher relative water content (RWC) and total soluble sugar content, and less cell membrane damage under drought stress conditions. The expression profiles showed different expression patterns with the use of different promoters. The codon-optimized ThIPK2 gene is a candidate gene to enhance wheat drought stress tolerance by genetic engineering.
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Current research progresses on calf rearing and nutrition in China
DIAO Qi-yu, ZHANG Rong, TU Yan
2017, 16 (
12
): 2805-2814. DOI:
10.1016/S2095-3119(17)61767-2
Abstract
(
748
)
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(262KB)(
118
)
Calves are the reserve forces for dairy cattle. Scientific rearing strategy of calves is the basis of efficient cattle breeding. However, many problems exist in current rearing systems of calves and restrict the sustainable development of dairy cattle in China. The absence of basic research is the most highlighted problem among them. Recent researches on growth performance, nutrient digestibility, blood biochemical indices and rumen development in calves were summarized in this paper. Additionally, the optimal levels of energy and protein in milk replacer and starter diets for calves were indicated, and limiting amino acids for calves fed different diets were discussed. A variety of additives, such as acidifiers, probiotics and polysaccharides, are regarded as promising alternatives to antibiotics to reduce disease in calves. Dietary supplementations of these additives have positive effects on growth and health of calves. However, studies on the nutrition of vitamins and minerals in calves have been seldom done, and deserve our further researches. To sum up, the postnatal period is one of the most critical “windows” for rumen manipulation and epigenetic regulation. Any changes from environments, especially early nutrition, may produce long-term effects on growth, health and milk yields in adult cattle.
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