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A rapid approach for isolating a single fungal spore from rice blast diseased leaves
FEI Li-wang, LU Wen-bo, XU Xiao-zhou, YAN Feng-cheng, ZHANG Li-wei, LIU Jin-tao, BAI Yuan-jun, LI Zhen-yu, ZHAO Wen-sheng, YANG Jun, PENG You-liang
2019, 18 (6): 1415-1418.   DOI: 10.1016/S2095-3119(19)62581-5
Abstract298)      PDF in ScienceDirect      
Single spore isolation is a fundamental approach in plant pathology and mycology to isolate and identify plant fungal pathogens from diseased samples.  However, routine single spore isolation procedure is time-consuming and has a high risk of contamination by other microorganisms.  In this study, we developed a rapid approach for isolating a single spore of the fungal pathogen, Pyricularia oryzae, from rice blast diseased leaves in the paddy field with low potential of contamination.  First, rice blast leaves with single lesions were selected in the paddy field, and a single lesion was cut out and pressed and dragged gently across the surface of water agar.  Next, a germinated single spore with a barely visible piece of agar was cut out of water agar with a dissecting needle under a stereomicroscope at approximately 120-fold magnification.  Last, the germinated single spore with water agar was transferred onto oatmeal tomato agar for growth and preservation.  Based on our experience, a skilled technician or student can successfully isolate single spore from over 150 independent diseased samples with nearly no contaminations in a single working day.  This approach is also suitable for isolating single spore from other fungal disease samples that produce abundant aerial conidia.
 
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Comparison of energy consumption and economic performance of organic and conventional soybean production - A case study from Jilin Province, China
ZHANG Li-wei, Til Feike, Jirko Holst, Christa Hoffmann, Reiner Doluschitz
2015, 14 (8): 1561-1572.   DOI: 10.1016/S2095-3119(15)61131-5
Abstract2340)      PDF in ScienceDirect      
Modern agriculture heavily depends on energy consumption, especially fossil energy, but intensive energy input increases the production cost for producers and results in environmental pollution. Organic agricultural production is considered a more sustainable system, but there is lack of scientific research on the energy consumption between organic and conventional systems in China. The analysis and comparison of energy use between the two systems would help decision-makers to establish economic, effective and efficient agricultural production. Thus, the objectives of the present study are to analyze energy inputs, outputs, energy efficiency, and economic benefits between organic and conventional soybean (Glycine max (L.) Merrill) production. A total of 24 organic farmers and 24 conventional farmers in Jilin Province, China, were chosen for investigation in 2010 production year. Total energy input was 71.55 GJ ha–1 and total energy output was 96.18 GJ ha–1 in the organic system, resulting in an energy efficiency (output/input) of 1.34. Total energy input was 9.37 GJ ha–1 and total energy output was 113.4 GJ ha–1 in the conventional system, resulting in the energy efficiency of 12.1. The huge discrepancy in energy inputs and respective efficiencies lies in the several times higher nutrient inputs in the organic compared to the conventional production system. Finally, the production costs ha–1 were 33% higher, and the net income ha–1 25% lower in the organic compared to the conventional soybean production system. It is recommended to improve fertilizer management in organic production to improve its energetic and economic performance.
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MicroRNAs Involved in the Pathogenesis of Phytophthora Root Rot of Soybean (Glycine max)
WANG Jing*, LIU Chun-yan*, ZHANG Li-wei, WANG Jia-lin, HU Guo-hua, DING Jun-jie , CHEN Qing-shan
2011, 10 (8): 1159-1167.   DOI: 10.1016/S1671-2927(11)60106-5
Abstract3391)      PDF in ScienceDirect      
Phytophthora root rot is one of the most prevalent diseases in the world, which can infect the seedlings and plants, withsubstantial negative impact on soybean yield and quality. MicroRNAs (miRNAs) are a class of post-transcriptionalregulators of gene expression during growth and development of organisms. A soybean disease-resistance varietySuinong 10 was inoculated with Phytophthora sojae race No. 1, and the specific miRNA resistant expression profile wasacquired by microarray for the first time. Different expressional miRNAs have been found after comparing the results ofthe treated sample with the control sample. Furthermore, the target genes of different expressional miRNAs were predicted.Two miRNAs, cbr-mir-241 and ath-miR854a, regulated the disease-resistance process directly through their targets, someenzymes. Another two miRNAs, gma-miR169a and ath-miR169h, participated in disease-resistance regulation as transcriptionfactors. Similarly, one miRNA, ptc-miR164f, has been reported to regulate the plant development. All of these studieswould be served as the foundation for exploring the resistance mechanism.
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