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Genome-wide identification and function analysis of the sucrose phosphate synthase MdSPS gene family in apple
ZHANG Li-hua, ZHU Ling-cheng, XU Yu, LÜ Long, LI Xing-guo, LI Wen-hui, LIU Wan-da, MA Feng-wang, LI Ming-jun, HAN De-guo
2023, 22 (7): 2080-2093.   DOI: 10.1016/j.jia.2023.05.024
Abstract241)      PDF in ScienceDirect      

Sucrose phosphate synthase (SPS) is a rate-limiting enzyme that works in conjunction with sucrose-6-phosphate phosphatase (SPP) for sucrose synthesis, and it plays an essential role in energy provisioning during growth and development in plants as well as improving fruit quality.  However, studies on the systematic analysis and evolutionary pattern of the SPS gene family in apple are still lacking.  In the present study, a total of seven MdSPS and four MdSPP genes were identified from the Malus domestica genome GDDH13 v1.1.  The gene structures and their promoter cis-elements, protein conserved motifs, subcellular localizations, physiological functions and biochemical properties were analyzed.  A chromosomal location and gene-duplication analysis demonstrated that whole-genome duplication (WGD) and segmental duplication played vital roles in MdSPS gene family expansion.  The Ka/Ks ratio of pairwise MdSPS genes indicated that the members of this family have undergone strong purifying selection during domestication.  Furthermore, three SPS gene subfamilies were classified based on phylogenetic relationships, and old gene duplications and significantly divergent evolutionary rates were observed among the SPS gene subfamilies.  In addition, a major gene related to sucrose accumulation (MdSPSA2.3) was identified according to the highly consistent trends in the changes of its expression in four apple varieties (‘Golden Delicious’, ‘Fuji’, ‘Qinguan’ and ‘Honeycrisp’) and the correlation between gene expression and soluble sugar content during fruit development.  Furthermore, the virus-induced silencing of MdSPSA2.3 confirmed its function in sucrose accumulation in apple fruit.  The present study lays a theoretical foundation for better clarifying the biological functions of the MdSPS genes during apple fruit development.

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The water-saving potential of using micro-sprinkling irrigation for winter wheat production on the North China Plain
ZHAI Li-chao, Lü Li-hua, DONG Zhi-qiang, ZHANG Li-hua, ZHANG Jing-ting, JIA Xiu-ling, ZHANG Zheng-bin
2021, 20 (6): 1687-1700.   DOI: 10.1016/S2095-3119(20)63326-3
Abstract175)      PDF in ScienceDirect      
The shortage of groundwater resources is a considerable challenge for winter wheat production on the North China Plain.  Water-saving technologies and procedures are thus urgently required.  To determine the water-saving potential of using micro-sprinkling irrigation (MSI) for winter wheat production, field experiments were conducted from 2012 to 2015.  Compared to traditional flooding irrigation (TFI), micro-sprinkling thrice with 90 mm water (MSI1) and micro-sprinkling four times with 120 mm water (MSI2) increased the water use efficiency by 22.5 and 16.2%, respectively, while reducing evapotranspiration by 17.6 and 10.8%.  Regardless of the rainfall pattern, MSI (i.e., MSI1 or MSI2) either stabilized or significantly increased the grain yield, while reducing irrigation water volumes by 20–40%, compared to TFI.  Applying the same volumes of irrigation water, MSI (i.e., MSI3, micro-sprinkling five times with 150 mm water) increased the grain yield and water use efficiency of winter wheat by 4.6 and 11.7%, respectively, compared to TFI.  Because MSI could supply irrigation water more frequently in smaller amounts each time, it reduced soil layer compaction, and may have also resulted in a soil water deficit that promoted the spread of roots into the deep soil layer, which is beneficial to photosynthetic production in the critical period.  In conclusion, MSI1 or MSI2 either stabilized or significantly increased grain yield while reducing irrigation water volumes by 20–40% compared to TFI, and should provide water-saving technological support in winter wheat production for smallholders on the North China Plain.
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