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Detection of antimicrobial resistance and virulence-related genes in Streptococcus uberis and Streptococcus parauberis isolated from clinical bovine mastitis cases in northwestern China
ZHANG Hang, YANG Feng, LI Xin-pu, LUO Jin-yin, WANG Ling, ZHOU Yu-long, YAN Yong, WANG Xu-rong, LI Hong-sheng
2020, 19 (11): 2784-2791.   DOI: 10.1016/S2095-3119(20)63185-9
Abstract118)      PDF in ScienceDirect      
The objectives of this study were to investigate antimicrobial resistance of Streptococcus uberis and Streptococcus parauberis isolated from cows with bovine clinical mastitis in China and to examine the distribution of resistance- and virulence-related gene patterns.  Antimicrobial susceptibility was determined by the E-test.  Genes encoding antimicrobial resistance and invasiveness factors were examined by PCR.  A total of 27 strains were obtained from 326 mastitis milk samples.  Streptococcus parauberis isolates (n=11) showed high resistance to erythromycin (90.9%), followed by tetracycline (45.5%), chloramphenicol (36.4%) and clindamycin (27.3%).  Streptococcus uberis isolates (n=16) were highly resistant to tetracycline (81.3%) and clindamycin (62.5%).  Both species were susceptible to ampicillin.  The most prevalent resistance gene in S. uberis was tetM (80.0%), followed by blaZ (62.5%) and ermB (62.5%).  However, tetM, blaZ, and ermB genes were only found in 27.3, 45.5, and 27.3%, respectively, of S. parauberis.  In addition, all of the isolates carried at least one selected virulence-related gene.  The most prevalent virulence-associated gene pattern in the current study was sua+pauA/skc+gapC+hasC detected in 22.2% of the strains.  One S. uberis strain carried 7 virulence-associated genes and belonged to the sua+pauA/skc+gapC+cfu+hasA+hasB+hasC pattern.  More than 59.3% of analysed strains carried 4 to 7 virulence-related genes.  Our findings demonstrated that S. parauberis and S. uberis isolated from clinical bovine mastitis cases in China exhibited diverse molecular ecology, and that the strains were highly resistant to antibiotics commonly used in the dairy cow industry.  The data obtained in the current study contribute to a better understanding of the pathogenesis of bacteria in mastitis caused by these pathogens, and the findings are relevant to the development of multivalent vaccines and targeted prevention procedures.
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Effects of Chlorination on Soil Chemical Properties and Nitrogen Uptake for Tomato Drip Irrigated with Secondary Sewage Effluent
LI Yan-feng, LI Jiu-sheng, ZHANG Hang
2014, 13 (9): 2049-2060.   DOI: 10.1016/S2095-3119(13)60692-9
Abstract1599)      PDF in ScienceDirect      
Chlorination is usually an economical method for treating clogging in drip emitters during sewage application. Appropriate assessment of the responses of soil and crop is essential for determining an optimal chlorination scheme. During 2008 to 2009, field experiments were conducted in a solar-heated greenhouse for tomato drip irrigated with secondary sewage effluent, to investigate the influences of chlorine injection intervals and levels on soil chemical properties and nitrogen uptake. Injection intervals ranging from two to eight weeks and injection concentrations ranging from 2 to 50 mg L-1 were used. A salinity factor and a nutrient factor were extracted from the pool of the nine soil chemical constituents using factor analysis method. The results demonstrated that chlorination practices increased the residual Cl in the soil, resulting in an increased salinity factor, especially for the frequent chlorination at a high injection concentration. Chlorination weakened the accumulation of nutrients factor in the upper soil layer. Nitrogen uptake of the tomato plants also was inhibited by the increased salinity in the upper soil layer caused by high chlorination levels. In order to reduce the unfavorable effect on soil chemical properties and nitrogen uptake, chlorination scheme with concentrations of lower than 20 mg L-1 was recommended.
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Tomato Yield and Quality and Emitter Clogging as Affected by Chlorination Schemes of Drip Irrigation Systems Applying Sewage Effluent
LI Jiu-sheng, LI Yan-feng, ZHANG Hang
2012, 12 (10): 1744-1754.   DOI: 10.1016/S1671-2927(00)8709
Abstract1163)      PDF in ScienceDirect      
Chlorination has been recognized as an efficient and economically favorable method for treating clogging in drip emitters caused by biological growth during sewage application. Further important criteria for determining an optimal chlorination scheme are the different responses of crops to the chloride added into the soil through chlorination. During two seasons in 2008 and 2009, field experiments were conducted in a solar-heated greenhouse with drip irrigation systems applying secondary sewage effluent to tomato plants to investigate the influences of chlorine injection intervals and levels on plant growth, yield, fruit quality, and emitter clogging. Injection intervals ranging from 2 to 8 wk and injection concentrations ranging 2-50 mg L-1 of free chlorine residual at the end of the laterals were used. For the 2008 experiments, the yield from the treatments of sewage application with chlorination was 7.5% lower than the yield from the treatment of sewage application without chlorination, while the yields for the treatments with and without chlorination were similar for the 2009 experiments. The statistical tests indicated that neither the chlorine injection intervals and concentrations nor the interactions between the two significantly influenced plant height, leaf area, or tomato yield for both years. The qualities of the fruit in response to chlorination were parameter-dependent. Chlorination did not significantly influence the quality of ascorbic acid, soluble sugar, or soluble acids, but the interaction between the chlorine injection interval and the chlorine concentration significantly influenced the levels of soluble solids. It was also confirmed that chlorination was an effective method for reducing biological clogging. These results suggested that chlorination is safe for a crop that has a moderate sensitivity to chlorine, like tomato, and can maintain a high level of performance in drip irrigation systems applying sewage effluent
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