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Transcriptomic and metabolomic analysis of changes in grain weight potential induced by water stress in wheat
Yanmei Gao, Maoya Jing, Meng Zhang, Zhen Zhang, Yuqing Liu, Zhimin Wang, Yinghua Zhang
2024, 23 (11): 3706-3722.   DOI: 10.1016/j.jia.2023.12.015
Abstract145)      PDF in ScienceDirect      
The sink strength of developing ovaries in wheat determines the grain weight potential.  The period from booting to the grain setting stage is critical for ovary growth and development and potential sink capacity determination.  However, the underlying regulatory mechanism during this period by which the wheat plant balances and coordinates the floret number and ovary/grain weight under water stress has not been clarified.  Therefore, we designed two irrigation treatments of W0 (no seasonal irrigation) and W1 (additional 75 mm of irrigation at the jointing stage) and analyzed the responses of the ovary/grain weight to water stress at the phenotypic, metabolomic, and transcriptomic levels.  The results showed that the W0 irrigation treatment reduced the soil water content, plant height, and green area of the flag leaf, thus reducing grain number, especially for the inferior grains.  However, it improved the grain weight of the superior and inferior grains as well as average grain weight at maturity, while the average ovary/grain weight and volume during –3 to 10 days after anthesis (DAA) also increased.  Transcriptomic analysis indicated that the genes involved in both sucrose metabolism and phytohormone signal transduction were prominently accelerated by the W0 treatment, accompanied by greater enzymatic activities of soluble acid invertase (SAI) and sucrose synthase (Sus) and elevated abscisic acid (ABA) and indole-3-acetic acid (IAA) levels.  Thus, the sucrose content decreased, while the glucose and fructose contents increased.  In addition, several TaTPP genes (especially TaTPP-6) were down-regulated and the IAA biosynthesis genes TaTAR1 and TaTAR2 were up-regulated under the W0 treatment before anthesis, which further increased the IAA level.  Collectively, water stress reduced the growth of vegetative organs and eliminated most of the inferior grains, but increased the ABA and IAA levels of the surviving ovaries/grains, promoting the enzymatic activity of Sus and degrading sucrose into glucose and fructose.  As a result, the strong sucrose utilization ability, the enhanced enzymatic activity of SAI and the ABA- and IAA-mediated signaling jointly increased the weight and volume of the surviving ovaries/grains, and ultimately achieved the trade-off between ovary/grain weight and number in wheat under water stress.


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Effects of micro-sprinkler irrigation and topsoil compaction on winter wheat grain yield and water use efficiency in the Huaibei Plain
Jinpeng Li, Siqi Wang, Zhongwei Li, Kaiyi Xing, Xuefeng Tao, Zhimin Wang, Yinghua Zhang, Chunsheng Yao, Jincai Li
DOI: 10.1016/j.jia.2025.02.020 Online: 18 February 2025
Abstract15)      PDF in ScienceDirect      

Increasing grain yield (GY) and water use efficiency (WUE) of winter wheat in the Huaibei Plain (HP) is essential.  However, the effects of micro-sprinkler irrigation and topsoil compaction after wheat seeds sowing on the GY and WUE are unclear.  Therefore, a two-year field experiment was conducted during the 2021–2023 winter wheat growing seasons with a total six treatments: rain-fed (RF), conventional irrigation (CI) and micro-sprinkler irrigation (MI), as well as topsoil compaction after seeds sowing under three irrigation methods (RFC, CIC, and MIC).  The two years’ results indicated that MI significantly increased GY compared to CI and RF, which averagely increased by 17.9 and 42.1%, respectively.  The increase in GY of MI was due to its significant increase in the number of spikes, kernels per spike, and grain weight.  Chlorophyll concentration in flag leaves of MI after anthesis stage was maintained higher levels than CI and RF, RF was the lowest.  This was due to the dramatically enhanced catalase and peroxidase activity and lower malondialdehyde content under MI.  Compared with RF and CI, MI significantly promoted dry matter remobilization and production after anthesis as well as its contribution to GY.  In addition, MI significantly boosted root growth, and root activity during grain filling stage was remarkably enhanced than CI and RF.  In 2021–2022, there was no significant difference in WUE between MI and RF, but the WUE of RF was significantly lower than MI in 2022–2023.  However, WUE in MI was significantly improved compared to CI, that averagely increased by 15.1 and 17.6% for the two years.  Topsoil compaction significantly increased GY and WUE under rain-fed conditions due to improved spike numbers and dry matter production.  Overall, topsoil compaction is advisable for enhancing GY and WUE in rain-fed conditions, whereas micro-sprinkler irrigation can be adopted to achieve high GY and WUE simultaneously in the HP.

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Faster and more wheat production governed by LED light in controlled environment agriculture
Xiaolei Guo, Zhimin Wang, Mingjie Li, Zhongyi Zhang, Xuzhang Xue, Yinghua Zhang, Li Gu
DOI: 10.1016/j.jia.2025.03.019 Online: 22 March 2025
Abstract5)      PDF in ScienceDirect      

Wheat (Triticum aestivum L.) is a major food crop grown worldwide; yet, field-grown wheat is generally restricted to only one generation per year and has a fluctuating yield, limiting wheat improvement and failing to meet future food demand.  To minimize generation time and increase total annually wheat production, five light regimens with varied day length and spectral distribution, including 12 h light/12 h dark+white light (P12W), 17 h light/7 h dark+white light (P17W), 22 h light/2 h dark+white light (P22W), 22 h light/2 h dark+red:green:blue light=6:3:2 (P22RGB), and 22 h light/2 h dark+red:blue light=6:1 (P22RB), were developed by adjusting the light emitting diodes (LEDs) in the controlled environment.  The results showed that controlled wheat agriculture illuminated by LED sources equipped with various day lengths and spectral distributions had the potential for “faster” and “more” grain production.  Prolonged day length (from 12 h to 17 h and then to 22 h) accelerated wheat development, particularly shortening the duration before flowering, and that the longer the prolonged time, the earlier the flowering.  However, 22 h day length (e.g., P22W treatment) would affect plant morphological traits, reduce dry matter accumulation, and result in a loss of yield-related components due to increased stress and disrupted pollen development.  Surprisingly, regulating the spectral distribution towards the red-light region under long-day conditions (e.g., P22RB treatment) could partially restore the grain yield of wheat.  The light regime with a rich red-light region contributed to dry matter accumulation, carbohydrate flow to reproductive tissues, and sporopollenin biosynthesis, resulting in improved plant morphology and grain yield in wheat.  Collectively, the optimized light regimes, represented by P17W and P22RB treatments in controlled environment agriculture, can produce 5-6 generations of wheat per year, yielding 3.16-5.87 kg m-2 yr-1, which is 3.59-6.68 times higher than field cultivation.  Thus, conducting appropriate LED light regimens is a favorable way to achieve the dual goals of “faster and more” in controlled wheat cultivation. 

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