Cadmium (Cd) contamination in wheat farmland is increasing at an alarming rate, posing threats to food security and public health.  Breeding and utilizing wheat varieties characterized by low Cd accumulation levels constitute an effective strategy in the battle against wheat Cd contamination.  The adoption of molecular marker-assisted approaches can greatly expedite the selection and enhancement of wheat varieties with low Cd accumulation.  Nonetheless, research concerning the genes associated with wheat cadmium accumulation remains scarce.  In this study, a high-density 660K SNP array was employed for conducting a genome-wide association study (GWAS) on the grain Cd concentration (GCdC), bioconcentration factor (BCF) and translocation factor (TF) in 175 wheat germplasms.  The findings revealed 401 significant SNPs identified across three diverse environments.  Linkage disequilibrium analysis revealed 30 core quantitative trait loci (QTLs) capable of reliably modulating wheat Cd accumulation phenotypes.  Through gene annotation, transcriptomics, and gene molecular features, four candidate genes (TraesCS7B02G000200, TraesCS4A02G035900, TraesCS4A02G040900, and TraesCS5D02G564000) were identified as potential constituents in the biological process of wheat Cd accumulation.  Furthermore, six wheat germplasms exhibiting low grain Cd accumulation were isolated, and two kompetitive allele specific PCR (KASP) markers conducive to breeding selection were developed.  These findings provide valuable genetic resources for cultivating wheat with low Cd accumulation and establish a foundation for understanding the molecular mechanisms underlying low Cd accumulation in wheat.  The candidate genes and KASP markers elucidated in this research have potential for effective use in genetic enhancement and marker-assisted selection in the breeding of wheat with low Cd accumulation.