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Journal of Integrative Agriculture
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Production of homeobox A10 gene transgenic pigs by somatic cell nuclear transfer
XIAO Qian, ZHAO Chang-zhi, LIN Rui-yi, LI Guang-lei, LI Chang-chu, WANG Hai-yan, XU Jing, XIE Sheng-song, YU Mei, ZHAO Shu-hong
2019, 18 (
5
): 1072-1079. DOI:
10.1016/S2095-3119(19)62582-7
Abstract
(
233
)
PDF in ScienceDirect
Homeobox A10 (
Hoxa10
) gene is one of the most important candidate genes associated with the reproductive performance of humans and mice. Overexpression of
Hoxa10
in mouse endometrium can increase litter size. Moreover,
Hoxa10
plays a key role in regulating the embryo implantation of sows. This study aimed to generate transgenic pigs using
Hoxa10
via somatic cell nuclear transfer (SCNT). We established seven
Hoxa10
-transgenic cell lines, and two of the cell lines were selected as nuclear donors for the transfer. A total of 1 270 cloned embryos were generated and transferred to five surrogate mothers (Landrace×Yorkshire). Eight cloned male piglets were produced including one with cryptorchidism. Six transgenic piglets grew up healthy and produced 56 offspring. Finally, we obtained six transgenic male pigs and 26 transgenic positive offspring that can be used to further study the regulatory mechanism of
Hoxa10
on the reproductive performance of pigs.
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Human Capital of Family and Social Mobility in Rural Areas - Evidence from China
ZHANG Jin-hua, YU Mei-lian, WU Fang-wei , CHEN Wei
2013, 12 (
10
): 1905-1915. DOI:
10.1016/S2095-3119(13)60599-7
Abstract
(
1482
)
PDF in ScienceDirect
This research focuses on the impact of family’s human capital on social mobility in China’s rural community. Empirical research is conducted based on data from surveying a typical rural community in the past 20 yr. The study indicates that social mobility in rural area is active in the past 20 yr, and the human capital of family, represented by primary labor’s education level, has played an essential role in mobility of low social class. Meanwhile, socio-economic development and the change of supply and demand in labor market dims the signaling role of degree education, but the impact of occupational training is increasingly remarkable. Therefore, the change from sole degree education to multi-leveled education including occupational education and training is a main way for China’s rural families in low class to realize social mobility.
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Spatio-Temporal Changes of Soil Salinity in Arid Areas of South Xinjiang Using Electromagnetic Induction
LI Xiao-ming, YANG Jing-song, LIU Mei-xian, LIU Guang-ming, YU Mei
2012, 12 (
8
): 1365-1376. DOI:
10.1016/S1671-2927(00)8667
Abstract
(
1364
)
PDF in ScienceDirect
The aim of this paper was to research the spatio-temporal changes in total soluble salt content (TS) in a typical arid region of South Xinjiang, China, where the climate is arid and soil salinization happens easily. The total soluble salt content was interpreted by measurements made in the horizontal mode with EM38 and EM31. The electromagnetic induction (EM) surveys were made three times with the apparent soil electrical conductivity (ECa) measurements taken at 3 873 locations in Nov. 2008, 4807 locations in Apr. 2009 and 6 324 locations in Nov. 2009, respectively. For interpreting the ECa measurements into total soluble salt content, calibtion sites were needed for EM survey of each time, e.g., 66 sites were selected in Nov. 2008 to measure ECa, and soils-core samples were taken by different depth layers of 0-10, 10-20 and 20-40 cm at the same time. On every time duplicate samples were taken at five sites to allevaite the local-scale variability, and soil temperatures in different layers through the profiles were also measured. Factors including TS, pH, water content, bulk density were analyzed by lab experiments. ECa calibration equations were obtained by linear regression analysis, which indicated that soil salinity was one primary concern to ECa with a determination coefficient of 0.792 in 0-10 cm layer, 0.711 in 10-20 cm layer and 0.544 in 20-40 cm layer, respectively. The maps of spatial distribution were predicted by Kriging interpolation, which showed that the high soil salinity was located near the drainage canal, which validated the trend effect caused by the irrigation canal and the drainage canal. And by comparing the soil salinity in different layers, the soluble salt accumulated to the top soil surface only in the area where the soil salinization was serious, and in the other areas, the soil salinity trended to increase from the top soil surface to 40 cm depth. Temporal changes showed that the soil salinity in November was higher than that in April, and the soil salinization trended to aggravate, especially in the top soil layer of 0- 10 cm.
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Effects of Irrigation Water Quality and Drip Tape Arrangement on Soil Salinity, Soil Moisture Distribution, and Cotton Yield (Gossypium hirsutum L.) Under Mulched Drip Irrigation in Xinjiang, China
LIU Mei-xian, LI Xiao-ming, YU Mei, WANG Jin
2012, 12 (
3
): 502-511. DOI:
10.1016/S1671-2927(00)8569
Abstract
(
1456
)
PDF in ScienceDirect
More and more attention is being focused on saline water utilization in irrigation due to the shortage of fresh water to agriculture in many regions. For purpose of reducing the risks of using of saline water for irrigation, the mechanism of soil moisture and salinity distribution and transport should be well understood for developing optimum management strategies. In this paper, field experiments were carried out at Junggar Basin, China, to study the effects of drip irrigation water quality and drip tape arrangement on distribution of soil salinity and soil moisture. Six treatments were designed, including two drip tape arrangement modes and three irrigation water concentration levels (0.24, 4.68, and 7.42 dS m-1). Results showed that, soil moisture content (SMC) directly beneath the drip tape in all treatments kept a relatively high value about 18% before boll opening stage; the SMC in the narrow strip in single tape arrangement (Ms) plot was obviously lower than that in the double tapes arrangement (Md) plot, indicating that less sufficient water was supplied under the same condition of irrigation depth, but there was no significant reduction in yield. Mulching had not significant influence on salt accumulation but the drip tape arrangement, under the same condition of irrigation water depth and quality, compared with Md, Ms reduced salt accumulation in root zone and brought about relatively high cotton yield.
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