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miR-27b-5p regulates chicken liver disease via targeting IRS2 to suppress the PI3K/AKT signal pathway
ZHAO Jing, WU Ya-mei, ZHANG Yao, TANG Shu-yue, HAN Shun-shun, CUI Can, TAN Bo, YU Jie, KANG Hou-yang, CHEN Guang-deng, MA Meng-gen, ZHU Qing, YIN Hua-dong
2023, 22 (11): 3500-3516.   DOI: 10.1016/j.jia.2023.04.010
Abstract220)      PDF in ScienceDirect      

The liver is a vital organ in chickens that performs a number of crucial physiological functions, including the storage of hepatic glycogen, protein synthesis, detoxification, and deoxidation.  The growth and metabolism of the liver are complex processes influenced by factors such as environment, diet, and genetics.  MicroRNAs (miRNAs), as post-transcriptional regulatory molecules, play a role in various biological processes.  There is growing evidence that miR-27b-5p plays a key role in the regulation of liver development and metabolism in various species.  However, its role in chicken livers has yet to be determined.  In our experiment, we found that chickens with fatty livers had significantly higher levels of serum triglyceride (TG) and total cholesterol (TC) compared to the normal chickens, while the control group had significantly higher levels of very low-density lipoprotein (VLDL) and serum hormones.  Further research showed that the mRNA of miR-27b-5p was highly expressed in fatty livers.  By exploring the function of miR-27b-5p in chicken livers, we discovered that it promotes lipogenesis, oxidative stress, and inflammatory responses, leading to hepatocyte apoptosis.  Our study also established the mechanism by which miR-27b-5p interacts with its target gene, and found that miR-27b-5p targets insulin receptor substrate 2 (IRS2) and modulates the PI3K/AKT signaling pathway.  Additionally, our investigation of IRS2 in chicken hepatocytes revealed that knocking down IRS2 has the same effects as overexpressing miR-27b-5p.  In conclusion, our study revealed that miR-27b-5p directly binds to IRS2, inhibiting the PI3K/AKT signaling pathway and causing steatosis, oxidative stress, inflammation, and apoptosis in chicken liver.

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Expression profiles and functional prediction of ionotropic receptors in Asian corn borer, Ostrinia furnacalis (Lepidoptera: Crambidae)
ZHANG Yu, YANG Bin, YU Jie, PANG Bao-ping, WANG Gui-rong
2022, 21 (2): 474-485.   DOI: 10.1016/S2095-3119(20)63427-X
Abstract181)      PDF in ScienceDirect      
Genes involved in chemosensation are essential for odorant-mediated insect behaviors.  Odorant receptors (ORs) bind and respond to pheromones and plant volatiles, regulating insect behaviors such as mating and host-plant selection, while ionotropic receptors (IRs), which are present at lower levels in insects than ORs, influence ion channels, especially in agricultural pests.  Asian corn borer, Ostrinia furnacalis, is the main pest of maize that causes huge economic losses in Asia.  Twenty-one OfurIRs have been identified, but none has been characterized.  In this study, tissue-specific expression profiling, phylogenetic analysis, and electroantennography (EAG) analysis were applied to characterize the evolution, expression, and the potential function of OfurIRs.  It was found that 20 OfurIRs were highly expressed in the antennae, except for OfurIR75p3, whereas 10 and nine OfurIRs were highly expressed in the proboscis and genitalia, respectively, indicating that these OfurIRs were functionally associated with feeding and oviposition.  EAG results showed that seven acids elicited responses in the antennae of O. furnacalis and that 2-oxopentanoic acid displayed a significant female-biased response.  Combined with the phylogenetic analysis, 10 OfurIRs in clade 4 were roughly predicted to be candidate receptors for 2-oxopentanoic acid and other tested acids.  These results provide basic information about OfurIRs and may help advance the knolwedge on the olfactory system of O. furnacalis
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The toxicological effect of dietary excess of saccharicterpenin, the extract of camellia seed meal, in piglets
WANG Man, YU Bing, HE Jun, YU Jie, LUO Yu-heng, LUO Jun-qiu, MAO Xiang-bin, CHEN Dai-wen
2020, 19 (1): 211-224.   DOI: 10.1016/S2095-3119(19)62789-9
Abstract127)      PDF in ScienceDirect      
Recently, saccharicterpenin extracted from Camellia oleifera seed meal has become a widely used feed supplement in animal husbandry.  In order to assess its safety, this study was designed to investigate the toxicity and histopathological effects of saccharicterpenin on piglets.  One hundred-fifty weaned pigs ((Yorkshire×Landrace)×Duroc), 75 males and 75 females with body weight (BW) of (7.35±0.29) kg, were randomly allotted to groups receiving diets supplemented with 0, 500, 1 000, 2 500 or 5 000 mg kg–1 saccharicterpenin for 70 d.  The diet with 500 mg kg–1 saccharicterpenin supplementation improved liver glutathione peroxidase (GSH-Px) activity, and the diet with 1 000 mg kg–1 saccharicterpenin supplementation improved liver glutathione S-transferase (GSH-S) activity in piglets on d 70 (P<0.05).  At 2 500 mg kg–1, saccharicterpenin in the diets reduced average daily feed intake (ADFI) and average daily gain (ADG) of piglets from d 1 to 35, damaged the cardiac tissue and liver on d 35, and decreased white blood cell counts (WBC), activities of catalase (CAT) and GSH-Px, and concentrations of glucose (GLU) and urea nitrogen (BUN) in the blood of piglets on d 70 (P<0.05).  In addition, diets with 5 000 mg kg–1 saccharicterpenin supplementation reduced ADFI, ADG and increased diarrhea rates of piglets from d 36 to 70, and decreased hemoglobin (HGB) concentration and activity of CAT in the blood of piglets on d 70 (P<0.05).  Moreover, at a rate of 5 000 mg kg–1, saccharicterpenin supplementation increased pancreas index on d 35 and hepatic index on d 70, and damaged cardiac tissue, liver and spleen during the whole experimental period (P<0.05).  These results suggested that dietary 500 mg kg–1 saccharicterpenin supplementation had beneficial effects on piglets, but excessive supplementation
(2 500 or 5 000 mg kg–1) of saccharicterpenin in the diets could lead to growth retardation, hematological abnormalities and organ injuries.
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Effect of dietary supplementation with mulberry (Morus alba L.) leaves on the growth performance, meat quality and antioxidative capacity of finishing pigs
ZENG Zhu, JIANG Jun-jie, YU Jie, MAO Xiang-bing, YU Bing, CHEN Dai-wen
2019, 18 (1): 143-151.   DOI: 10.1016/S2095-3119(18)62072-6
Abstract311)      PDF in ScienceDirect      
The present study was conducted to evaluate the effect of dietary mulberry (Morus alba L.) leaves powder (MLP) supplementation on meat quality of finishing pigs.  A total of 40 Duroc×Landrace×Yorkshire pigs (initial body weight of (40.5±0.63) kg) were randomly allotted into two treatments, fed either with control diet or 15% MLP diet for 85 d.  The results showed that MLP diet decreased (P≤0.05) average daily gain (ADG) and increased (P<0.05) feed/gain ratio (F/G) in the finishing and whole period.  MLP diet also decreased (P<0.05) carcass weight, dressing percentage, last rib and average backfat depth.  However, MLP diet increased (P<0.05) intramuscular fat (IMF) content, decreased (P<0.05) shear force, cooking loss and drip loss.  In addition, MLP diet increased (P<0.05) total antioxidative capacity, glutathione peroxidase and tended (P<0.10) to increase total superoxide dismutase in serum.  In longissimus thoracis, myosin heavy chain (MyHC) I and IIa mRNA levels were increased (P≤0.05) for MLP diet.  In conclusion, 15% MLP supplementation reduced the growth performance and carcass traits, but improved meat quality of finishing pigs possibly through the change of myofiber characteristics, enhancement of antioxidative capacity and increase of IMF. 
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