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Determining nitrogen status and quantifying nitrogen fertilizer requirement using a critical nitrogen dilution curve for hybrid indica rice under mechanical pot-seedling transplanting pattern
YAO Bo, HE Hai-bing, XU Hao-cong, ZHU Tie-zhong, LIU Tao, KE Jian, YOU Cui-cui, ZHU De-quan, WU Li-quan
2021, 20 (6): 1474-1486.   DOI: 10.1016/S2095-3119(21)63622-5
Abstract107)      PDF in ScienceDirect      
Field experiments of nitrogen (N) treatment at five different application rates (0, 75, 150, 225, and 300 kg ha−1) were conducted under pot-seedling mechanical transplanting (PMT) in 2018 and 2019.  Two high-quality and high-yielding hybrids of indica rice, Huiliangyou 898 and Y Liangyou 900, were used in this study.  The N nutrition index (NNI) and accumulated N deficit (Nand), used to assess the N nutrition status in real-time, were calculated for the indica cultivars under PMT with a critical nitrogen concentration (Nc) dilution model based on shoot dry matter (DM) during the whole rice growth stage.  The relationships between NNI and Nand with relative yield (RY) were determined, and accurate N application schemes were developed for hybrids indica rice under PMT.  The results indicated that high application rate of N-fertilizer significantly increased the concentrations of shoot DM and N in aboveground organs during the observed stages in the two cultivars for two years (P<0.05).  The Nc dilution model of hybrid indica cultivars was Nc=4.02DM−0.42 (R2=0.97) combining the two cultivars under PMT.  Root-mean-square error and normalized root-mean-square error of the curve verification were 0.23 and 10.61%, respectively.  The NNI and Nand ranged from 0.58 to 1.31 and 109 to –55 kg ha−1, respectively, in the two cultivars for all N treatments.  NNI showed a linear relationship with Nand during the entire growth stage (0.53<R2<0.99, P<0.01).  In addition, NNI showed a linear-plateau relationship with RY (0.73<R2<0.92, P<0.01) throughout the observed stages.  These results suggest that the models can accurately diagnose the N-nutrition status and support effective N-fertilizer management in real-time for hybrid indica rice under PMT.
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Development of an automatic monitoring system for rice light-trap pests based on machine vision
YAO Qing, FENG Jin, TANG Jian, XU Wei-gen, ZHU Xu-hua, YANG Bao-jun, Lü Jun, XIE Yi-ze, YAO Bo, WU Shu-zhen, KUAI Nai-yang, WANG Li-jun
2020, 19 (10): 2500-2513.   DOI: 10.1016/S2095-3119(20)63168-9
Abstract104)      PDF in ScienceDirect      
Monitoring pest populations in paddy fields is important to effectively implement integrated pest management.  Light traps are widely used to monitor field pests all over the world.  Most conventional light traps still involve manual identification of target pests from lots of trapped insects, which is time-consuming, labor-intensive and error-prone, especially in pest peak periods.  In this paper, we developed an automatic monitoring system for rice light-trap pests based on machine vision.  This system is composed of an intelligent light trap, a computer or mobile phone client platform and a cloud server.  The light trap firstly traps, kills and disperses insects, then collects images of trapped insects and sends each image to the cloud server.  Five target pests in images are automatically identified and counted by pest identification models loaded in the server.  To avoid light-trap insects piling up, a vibration plate and a moving rotation conveyor belt are adopted to disperse these trapped insects.  There was a close correlation (r=0.92) between our automatic and manual identification methods based on the daily pest number of one-year images from one light trap.  Field experiments demonstrated the effectiveness and accuracy of our automatic light trap monitoring system.
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Identification and fine mapping of a major QTL, qGPC4D, for grain protein content using wheat–Aegilops tauschii introgression lines
Yijun Wang, Jinhao Han, Tenglong Zhang, Mengjia Sun, Hongyu Ren, Cunyao Bo, Yuqing Diao, Xin Ma, Hongwei Wang, Xiaoqian Wang
DOI: 10.1016/j.jia.2024.07.029 Online: 19 July 2024
Abstract32)      PDF in ScienceDirect      

Wheat is a staple cereal crop that is crucial for food security and human health.  Improving wheat quality has become an essential task for breeders to meet escalating market demand.  In this study, a set of wheat-Aegilops tauschii introgression lines was developed from a cross between the high-yielding wheat variety Jimai 22 and Ae. tauschii Y215.  A high-density genetic map containing 2,727 single nucleotide polymorphisms (SNPs) was constructed using a 55K SNP array to conduct quantitative trait loci (QTL) analysis for grain quality-related traits.  Eight QTL were identified for grain protein content (GPC), starch content, and wet gluten content in the two environments.  Among them, a major and environmentally stable QTL, qGPC4D, for GPC was identified, with favorable alleles contributed by Ae. tauschii Y215.  Subsequently, qGPC4D was narrowed down to a 9.88 Mb physical interval through further fine mapping utilizing the introgression lines.  Additionally, three linked SNP of qGPC4D were converted into high-throughput kompetitive allele-specific PCR (KASP) markers and validated in the introgression population.  These findings offer promising candidate genes, elite introgression lines, and KASP markers for wheat high-quality breeding. 

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