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Application of virus-induced gene silencing for identification of FHB resistant genes
FAN Yan-hui, HOU Bing-qian, SU Pei-sen, WU Hong-yan, WANG Gui-ping, KONG Ling-rang, MA Xin, WANG Hong-wei
2019, 18 (
10
): 2183-2192. DOI:
10.1016/S2095-3119(18)62118-5
Abstract
(
136
)
PDF in ScienceDirect
Virus-induced gene silencing (VIGS) showed several advantages to identify gene functions such as short experimental cycle, more broad hosts, etc. In this study, the feasibility and efficiency of employing Barley stripe mosaic virus (BSMV)-based VIGS system to evaluate Fusarium head blight (FHB) resistance were explored in wheat. With variable conditions tested, it showed that the maximal silencing efficiency 78% on spike was obtained when the recombinant BSMV was inoculated on flag leaf at flagging stage. However, the plant may reduce its own immunity to FHB when inoculated with BSMV. To induce this impact, different
Fusarium graminearum
strains were tested and SF06-1 strain was selected for FHB resistance evaluation. Using this system,
TaAOC
,
TaAOS
, and
TaOPR3
involved in jasmonic acid (JA) signaling pathway were identified to positively regulate FHB resistance, which was underpinned by the results when silencing TaAOS in wheat by stable transgenic plants.
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Multi-functional roles of
TaSSI2
involved in Fusarium head blight and powdery mildew resistance and drought tolerance
HU Li-qin, MU Jing-jing, SU Pei-sen, WU Hong-yan, YU Guang-hui, WANG Gui-ping, WANG Liang, MA Xin, LI An-fei, WANG Hong-wei, ZHAO Lan-fei, KONG Ling-rang
2018, 17 (
2
): 368-380. DOI:
10.1016/S2095-3119(17)61680-0
Abstract
(
692
)
PDF in ScienceDirect
The mutation of the gene encoding a stearoyl-acyl carrier protein fatty acid desaturase (
ssi2
) has been proved to enhance pathogen resistance in several plants, while it’s potential to regulate biotic and abiotic stresses in wheat is still unclear. In this study, we cloned
TaSSI2
gene in wheat and provided several evidences of its involvement in multiple biological functions. By using barley stripe mosaic virus (BSMV)-induced gene silencing (VIGS) in wheat, it was found that
TaSSI2
negatively regulated both powdery mildew and Fusarium head blight (FHB) resistance, which was consistent with the phenotype observed in knock-out mutants of Kronos. The expression of
TaSSI2
was down-regulated by
in
vitro
treatments of methyl jasmonate (MeJA), but positively regulated by salicylic acid (SA) and abscisic acid (ABA), implying the cross-talk between different hormone signaling pathways involved in wheat to regulate biotic stresses is still to be elucidated. Furthermore, the up-regulated expression of
PR4
and
PR5
indicated that
TaSSI2
probably regulated FHB resistance by depressing the SA signaling pathway in wheat. In addition, the over-expression of
TaSSI2
increased the content of linolenic acid (18:3) and subsequently enhanced drought tolerance of transgenic
Brachypodium
. This phenomenon might be associated with its subcellular localization in the whole cytosol, partly overlapping with Golgi apparatus and the secreted vesicles. As a stearoyl-acyl carrier protein fatty acid desaturase,
TaSSI2
was proposed to be involved in cell lipid metabolism and carried targets out of the cell from membrane or wax synthesis, resulting in enhanced drought tolerance in plant.
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QTL effects and epistatic interaction for flowering time and branch number in a soybean mapping population of Japanese×Chinese cultivars
YANG Guang, ZHAI Hong, WU Hong-yan, ZHANG Xing-zheng, Lü Shi-xiang, WANG Ya-ying, LI Yu-qiu, HU Bo, WANG Lu, WEN Zi-xiang, WANG De-chun, WANG Shao-dong, Kyuya Harada, XIA Zheng-jun, XIE Fu-ti
2017, 16 (
09
): 1900-1912. DOI:
10.1016/S2095-3119(16)61539-3
Abstract
(
877
)
PDF in ScienceDirect
Flowering time and branching type are important agronomic traits related to the adaptability and yield of soybean. Molecular bases for major flowering time or maturity loci,
E1
to
E4
, have been identified. However, more flowering time genes in cultivars with different genetic backgrounds are needed to be mapped and cloned for a better understanding of flowering time regulation in soybean. In this study, we developed a population of Japanese cultivar (Toyomusume)×Chinese cultivar (Suinong 10) to map novel quantitative trait locus (QTL) for flowering time and branch number. A genetic linkage map of a F
2
population was constructed using 1 306 polymorphic single nucleotide polymorphism (SNP) markers using Illumina SoySNP8k iSelect BeadChip containing 7 189 (SNPs). Two major QTLs at
E1
and
E9
, and two minor QTLs at a novel locus,
qFT2_1
and at
E3
region were mapped. Using other sets of F
2
populations and their derived progenies, the existence of a novel QTL of
qFT2_1
was verified.
qBR6_1
, the major QTL for branch number was mapped to the proximate to the E1 gene, inferring that
E1
gene or neighboring genetic factor is significantly contributing to the branch number.
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Functional analysis of a wheat pleiotropic drug resistance gene involved in Fusarium head blight resistance
WANG Gui-ping, HOU Wen-qian, ZHANG Lei, WU Hong-yan, ZHAO Lan-fei, DU Xu-ye, MA Xin, LI An-fei, WANG Hong-wei, KONG Ling-rang
2016, 15 (
10
): 2215-2227. DOI:
10.1016/S2095-3119(16)61362-X
Abstract
(
1708
)
PDF in ScienceDirect
The pleiotropic drug resistance (PDR) sub-family of adenosine triphosphate (ATP)-binding cassette (ABC) transporter had been reported to participate in diverse biological processes of plant. In this study, we cloned three novel PDR genes in Fusarium head blight (FHB) resistant wheat cultivar Ning 7840, which were located on wheat chromosomes 6A, 6B and 6D. In phylogeny, these genes were members of cluster I together with
AePDR7
and
BdPDR7
. Subcellular localization analysis showed that
TaPDR7
was expressed on the plasmalemma. The quantitative real time PCR (RT-PCR) analysis showed that this gene and its probable orthologues in chromosomes 6B and 6D were both up-regulated sharply at 48 h after infected by
Fusarium graminearum
and
trichothecene deoxynivalenol
(DON) in spike. When knocking down the transcripts of all
TaPDR7
members by barely stripe mosaic virus-induced gene silencing (BSMV-VIGS) system, it could promote the
F. graminearum
hyphae growth and made larger pathogen inoculation points in Ning 7840, which suggested that
TaPDR7
might play an important role in response to
F. graminearum
. Although salicylic acid (SA), methyl jasmonate (MeJA) and abscisic acid (ABA) had been reported to possibly regulate wheat FHB resistance, here, we found that the three members of
TaPDR7
were negatively regulated by these three hormones but positively regulated by indoleacetic acid (IAA).
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