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Preharvest application of melatonin induces anthocyanin accumulation and related gene upregulation in red pear (Pyrus ussuriensis)
SUN Hui-li, WANG Xin-yue, SHANG Ye, WANG Xiao-qian, DU Guo-dong, LÜ De-guo
2021, 20 (8): 2126-2137.   DOI: 10.1016/S2095-3119(20)63312-3
Abstract200)      PDF in ScienceDirect      
Anthocyanins are important components in the peel of red pears and contribute to the appearance of the fruit.  Melatonin application is known to affect anthocyanin biosynthesis, but the effect of preharvest melatonin application on fruit coloration remains largely unknown.  The objective of this study was to determine the effects of preharvest melatonin application on pigmentation, phenolic compounds, and the expression of related genes in Nanhong pear (Pyrus ussuriensis).  The applications were performed during the pre-color-change period by spraying 50 or 200 μmol L–1 of melatonin on fruits.  We found that treatment with melatonin had a significant effect on color development.  The concentrations of anthocyanins and favonols were enhanced by melatonin treatment, whereas hydroxycinnamate and favanol concentrations were reduced.  Quantitative real-time PCR analyses indicated that the transcription levels for most anthocyanin biosynthetic genes and anthocyanin-related transcription factors were induced by melatonin.  Melatonin application also stimulated the expression of melatonin biosynthesis-related genes and consequently caused an increase in endogenous melatonin concentration.  These results provide insights into melatonin-induced fruit coloration and will facilitate the application of exogenous melatonin in agriculture.
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Effects of light intensity on photosynthesis and photoprotective mechanisms in apple under progressive drought
MA Ping, BAI Tuan-hui, WANG Xiao-qian, MA Feng-wang
2015, 14 (9): 1755-1766.   DOI: 10.1016/S2095-3119(15)61148-0
Abstract2203)      PDF in ScienceDirect      
The effects of light intensity on photosynthesis and photoprotective mechanisms under progressive drought were studied on apple trees (Malus domestica Borkh.) Fuji. The potted trees were exposed to drought stress for 12 days and different light conditions (100, 60 and 25% sunlight). During the progressive drought, the relative water content (RWC) in leaf declined and was faster in full light than in 60 and 25% sunlight. However, the decrease in the net photosynthetic rate (Pn), stomatal conductance (Gs) and Rubisco activity were slower under 100% sunlight condition than other light conditions. After the 6 days of drought, the maximum PSII quantum yield (Fv/Fm), the capacity of electrons move beyond QA − (1–VJ) and electron move from intersystem to PSI acceptor side (1–VI)/(1–VJ) decreased, with greater decline extent in brighter light. While RWCs were >75%, the variations in different light intensities of Gs and Rubisco activity at identical RWC, suggested the direct effects of light. While the little difference in the state of photosynthetic electron transport chain among tested light intensities indicates the results of faster water loss rate of light. Our results also demonstrated that the enhancement the de-epoxidations of xanthophyll cycle, activities of ascorbate peroxidase (APX) and catalase (CAT) were directly regulated by light intensity. While the higher photorespiration rate (Pr) under stronger light condition was mainly caused by faster water loss rate of light.
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