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Inheritance of steroidal glycoalkaloids in potato tuber flesh
PENG Zhen, WANG Pei, TANG Die, SHANG Yi, LI Can-hui, HUANG San-wen, ZHANG Chun-zhi
2019, 18 (10): 2255-2263.   DOI: 10.1016/S2095-3119(19)62718-8
Abstract154)      PDF in ScienceDirect      
Potato (Solanum tuberosum L.) is the third most important food crop worldwide after wheat and rice in terms of human consumption.  A critical domestication trait for potato was the decrease of toxic steroidal glycoalkaloids (SGAs) in tuber flesh.  Here, we used a diploid F2 segregating population derived from a cross between S. tuberosum and the wild potato species Solanum chacoense to map the quantitative trait loci (QTLs) associated with the regulation of SGAs content in tuber flesh.  In a three-year study, we identified two QTLs on chromosomes 2 and 8 affecting SGAs content in tuber flesh.  The QTL on chromosome 8 harbors 38 genes that are co-expressed with the GLYCOALKALOID METABOLISM genes.  These findings lay the foundation for exploiting the genes controlling SGAs content in tuber flesh and they provide a theoretical basis for the use of wild germplasm in potato breeding.
 
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Design and selection of an artificial diet for the coconut black-headed caterpillar, Opisina arenosella, based on orthogonal array analysis
JIN Tao, LIN Yu-ying, JIN Qi-an, WEN Hai-bo, PENG Zheng-qiang
2018, 17 (12): 2758-2757.   DOI: 10.1016/S2095-3119(17)61889-6
Abstract252)      PDF (412KB)(278)      
Opisina arenosella has been an outbreak pest of coconut trees in southern China since 2013.  To develop efficient control methods for this invasive pest, adequate rearing protocols are desirable.  In this study, an orthogonal array of artificial diets with 11 factors at 3 levels was deployed for both 2nd–4th and 5th–6th instar larvae of O. arenosella.  Biological parameters including survival time of larvae, development time from larva to pupa, pupation rate, emergence rate, and pupal weight were monitored to reveal the most important components in the diet formulas.  Biological parameters in O. arenosella were most affected by brewer’s yeast, sucrose, ascorbic acid, and wheat germ.  Statistical analysis indicated that different diet combinations supported optimum performance of biological parameters for 2nd–4th and 5th–6th instar larvae.  The validity of the optimization predicted by the orthogonal array analysis was confirmed in a follow-up bioassay with similar optimized diets for both 2nd–4th and 5th–6th instar larvae.  The optimal artificial diet has great potential for the mass rearing technique, and can provide valuable results for using parasitoids in biological control of O. arenosella.
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Effects of different nitrogen fertilizer management practices on wheat yields and N2O emissions from wheat fields in North China
LIU Ya-nan, LI Ying-chun, PENG Zheng-ping, WANG Yan-qun, MA Shao-yun, GUO Li-ping, LIN Er-da, HAN Xue
2015, 14 (6): 1184-1191.   DOI: 10.1016/S2095-3119(14)60867-4
Abstract2177)      PDF in ScienceDirect      
Nitrogen (N) is one of the macronutrients required for plant growth, and reasonable application of N fertilizers can increase crop yields and improve their quality. However, excessive application of N fertilizers will decrease N use efficiency and also lead to increases in N2O emissions from agricultural soils and many other environmental issues. Research on the effects of different N fertilizer management practices on wheat yields and N2O emissions will assist the selection of effective N management measures which enable achieving high wheat yields while reducing N2O emissions. To investigate the effects of different N management practices on wheat yields and soil N2O emissions, we conducted field trials with 5 treatments of no N fertilizer (CK), farmers common N rate (AN), optimal N rate (ON), 20% reduction in optimal rate+dicyandiamide (ON80%+DCD), 20% reduction in optimal rate+nano-carbon (ON80%+NC). The static closed chamber gas chromatography method was used to monitor N2O emissions during the wheat growing season. The results showed that there were obvious seasonal characteristics of N2O emissions under each treatment and N2O emissions were mainly concentrated in the sowing- greening stage, accounting for 54.6–68.2% of the overall emissions. Compared with AN, N2O emissions were decreased by 23.1, 45.4 and 33.7%, respectively, under ON, ON80%+DCD and ON80%+NC, and emission factors were declined by 22.2, 66.7 and 33.3%, respectively. Wheat yield was increased significantly under ON80%+DCD and ON80%+NC by 12.3 and 11.9%, respectively, relative to AN while there was no significant change in yield in the ON treatment. Compared with ON, overall N2O emissions were decreased by 29.1 and 13.9% while wheat yields improved by 18.3 and 17.9% under ON80%+DCD and ON80%+NC, respectively. We therefore recommend that ON80%+DCD and ON80%+NC be referred as effective N management practices increasing yields while mitigating emissions.
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TheAbundance andPopulationDynamics of Leptocybe invasa (Hymenoptera: Eulophidae) Galls on Eucalyptus spp. in China
ZHU Fang-li, REN Shun-xiang, QIU Bao-li, HUANG Zhen , PENG Zheng-qiang
2012, 12 (12): 2116-2123.   DOI: 10.1016/S1671-2927(00)8750
Abstract1298)      PDF in ScienceDirect      
The blue gum chalcid, Leptocybe invasa Fisher & La Salle, invaded China in 2007 and has subsequently caused substantial damage to eucalyptus trees. In the current paper, we investigated the susceptibility of 10 Eucalyptus spp. and Eucahetus dunnii to L. invasa in the field, determined the density of galls as well as the gall volume on these tree species, and monitored the population dynamics of wasps in Hainan and Guangdong provinces of China. The order of susceptibility to L. invasa was Eucalyptus urophylla×Eucalyptus camaldulensis>E. urophylla (coppices)>Eucalyptus exserta> Eucalyptus grandis×E. urophylla in Hainan, and Eucalyptus propinqua>Eucalyptus saligna>E. exserta>Eucalyptus microcorys>Eucahetus dunnii>E. camaldulensis>Eucalyptus tereticornis>Eucalyptus robust in Guangdong, China. Although L. invasa generally damages the midribs and petioles of young leaves and the tender bark of twigs of eucalyptus, galls were not observed on leaves of E. microcorys, E. camaldulensis, or E. dunnii. Gall volume significantly differed among the tree species, and gall volume and wasp number were positively correlated. In Dongfang, Hainan Province, the overwintering period of L. invasa emergencing through the year was from the end of December to March of the next year, and the number of population was the greatest on E. urophylla×E. camaldulensis, and the smallest on E. grandis×E. urophylla. In Guangzhou, Guangdong Province, L. invasa hardly emerged in winter from December to June of the next year, and the population was the greatest on E. propinqua, and the smallest on E. microcorys.
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