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Effects of selenium source and level on growth performance, antioxidative ability and meat quality of broilers
WANG Chuan-long, XING Guan-zhong, WANG Li-sai, LI Su-fen, ZHANG Li-yang, LU Lin, LUO Xu-gang, LIAO Xiu-dong
2021, 20 (
1
): 227-235. DOI:
10.1016/S2095-3119(20)63432-3
Abstract
(
169
)
PDF in ScienceDirect
This experiment was conducted to investigate the effects of selenium (Se) source and level on growth performance, carcass traits, antioxidative ability and meat quality of broilers. A total of 320 one-d-old Arbor Acres commercial broilers were randomly assigned to 1 of 5 treatments with 8 replicates in a completely randomized design involving a 2×2 factorial arrangement of treatments plus one Se-unsupplemented basal diet control for 42 d. The two Se sources were sodium selenite and Se yeast, and the two supplemental Se levels were 0.20 and 0.40 mg Se kg
–1
. The results showed that broilers fed the Se-supplemented diets had higher (P<0.05) average daily gain and average daily feed intake from 22 to 42 d of age, eviscerated yield and abdominal fat percentages, Se concentrations and glutathione peroxidase (GSH-Px) activities in breast and thigh muscles on d 42, and lower (
P
<0.05) feed/gain from 1 to 21 and 22 to 42 d of age, mortality from 22 to 42 d of age and malondialdehyde (MDA) concentration in thigh muscle on d 42 than those fed the control diet. Broilers fed the diets supplemented with Se yeast had higher (
P
<0.05) pH value and lower (
P
<0.05) shear force in thigh muscle than those fed the diets supplemented with sodium selenite. Additionally, broilers fed the diets supplemented with 0.40 mg Se kg
–1
had lower (
P
<0.05) shear force in thigh muscle and higher (
P
<0.05) GSH-Px activities in breast and thigh muscles than those fed the diets supplemented with 0.20 mg Se kg
–1
. Furthermore, broilers fed the diet supplemented with Se yeast at 0.40 mg Se kg
–1
had higher (
P
<0.05) Se concentrations in breast and thigh muscles than those fed the diet supplemented with Se yeast at 0.20 mg Se kg
–1
, but no differences (
P
<0.05) were observed in these indices of broilers fed the diets supplemented with sodium selenite between 0.20 and 0.40 mg Se kg
–1
. The results from the present study indicated that supplemental Se could increase the growth performance, muscle Se concentration and antioxidative ability of broilers; and the Se from Se yeast was more effective than the Se from sodium selenite in improving meat quality of broilers.
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Kinetics of selenium absorption in ligated small intestinal loops of chicks
LIU Guo-qing, ZHANG Shu-min, AN Zhi-min, FENG Yan-zhong, DONG Xue-yu, LI Su-fen, LU Lin, ZHANG Li-yang, WANG Run-lian, LUO Xu-gang, LIAO Xiu-dong
2020, 19 (
8
): 2095-2102. DOI:
10.1016/S2095-3119(20)63194-X
Abstract
(
135
)
PDF in ScienceDirect
Selenium (Se) is an essential trace element that has a large number of biological functions for broilers. However, the absorption kinetics of Se from sodium selenite in the small intestine of broilers remains unclear. Therefore, two experiments were conducted with 28-d-old commercial male broilers to study the kinetics of Se absorption in ligated small intestinal segments. In experiment 1, the Se absorption in duodenal, jejunal, and ileal segments at different post-perfusion time points (0, 20, 40, 60, 80, 100 and 120 min) were compared. In experiment 2, a kinetic study of Se absorption was conducted with the duodenal, jejunal, and ileal loops perfused with solutions containing 0, 0.0375, 0.075, 0.15, 0.30, or 0.60 μg mL
–1
of Se as sodium selenite, and Se contents in perfusates were determined at 100 min after perfusion. The results from experiment 1 showed that the Se absorption increased in an asymptotic response (
P
<0.0001) to post-perfusion time within 120 min in all the small intestinal segments, but increased linearly (
P
<0.0001) at less than 100 min after perfusion in duodenal and ileal segments, while more than 96.0% of the maximum Se absorption occurred at 100 min after perfusion in each small intestinal segment. In experiment 2, there was no difference (
P
>0.05) in the Se absorption rate among different ligated small intestinal segments perfused with solutions containing 0.0375–0.15 μg mL
–1
of Se, however, the Se absorption rate was higher (
P
<0.05) in the jejunum than that in the duodenum perfused with solutions containing 0.30–0.60 μg mL
–1
of Se. The kinetic curves of Se absorption demonstrated that the Se absorption was a saturated carrier-mediated process in the duodenum, and the maximum absorption rate was 1 271 pg min
–1
cm
–1
; whereas the Se absorptions were a non-saturated diffusion process in the jejunum and ileum, and the diffusive constants were 2 107 and 1 777 cm
2
min
–1
, respectively. The results from the present study indicate that the jejunum is the main Se absorption site, and the Se absorption is a saturated carrier-mediated process in the duodenum, but a non-saturated diffusion process in the jejunum and ileum of broilers.
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Effect of dietary calcium or phosphorus deficiency on bone development and related calcium or phosphorus metabolic utilization parameters of broilers from 22 to 42 days of age
YANG Yun-feng, XING Guan-zhong, LI su-fen, SHAO Yu-xin, ZHANG Li-yang, LU Lin, LUO Xu-gang, LIAO Xiu-dong
2020, 19 (
11
): 2775-2783. DOI:
10.1016/S2095-3119(20)63302-0
Abstract
(
79
)
PDF in ScienceDirect
This experiment was conducted to investigate the effect of dietary calcium (Ca) or phosphorus (P) deficiency on bone development and related Ca or P metabolic utilization parameters of broilers from 22 to 42 days of age based on our previous study, which indicated that dietary Ca or P deficiency impaired the bone development by regulating related Ca or P metabolic utilization parameters of broilers from 1 to 21 days of age. A total of 504 one-day-old Arbor Acres male broilers were randomly assigned to 1 of 4 treatments with 7 replicates in a completely randomized design, and fed the normal control and Ca- or P-deficient diets from 1 to 21 days of age. At 22 days of age, the broilers were further fed the normal control diet (0.90% Ca+0.35% non-phytate P (NPP)), the P-deficient diet (0.90% Ca+0.18% NPP), the Ca-deficient diet (0.30% Ca+0.35% NPP) or the Ca and P-deficient diet (0.30% Ca+0.18% NPP), respectively. The results showed that dietary Ca or P deficiency decreased (
P
<0.05) tibia bone mineral density (BMD), bone breaking strength (BBS), ash content, tibia ash Ca content and serum P content on days 28 and 42, but increased (
P
<0.05) tibia alkaline phosphatase (ALP) activity of broilers on day 42 compared with the control group. Furthermore, the broilers fed the P-deficient diet had the lowest (
P
<0.05) tibia BMD, BBS, ash content, serum P content and the highest (
P
<0.05) serum Ca content on day 28 compared with those fed the Ca-deficient or Ca and P-deficient diets. The results from the present study indicated that the bone development and related Ca or P metabolic utilization parameters of broilers were the most sensitive to dietary P deficiency, followed by dietary Ca deficiency or Ca and P-deficiency; dietary Ca or P deficiency impaired the bone development possibly by regulating serum Ca and P contents as well as tibia Ca content and ALP activity of broilers from 22 to 42 days of age.
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