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Optimization of slow-release fertilizer application improves lotus rhizome quality by affecting the physicochemical properties of starch

ZHAO Shu-ping, DENG Kang-ming, ZHU Ya-mei, JIANG Tao, WU Peng, FENG Kai, LI Liang-jun
2023, 22 (4): 1045-1057.   DOI: 10.1016/j.jia.2023.01.005
Abstract233)      PDF in ScienceDirect      

To achieve the dual goals of high yield and good quality with low environmental costs, slow-release fertilizer (SRF) has been widely used in lotus cultivation as new type of fertilizer instead of traditional nitrogen fertilizer.  However, the optimal amount of SRF and how it would promote lotus rhizome quality remain unclear.  This study was designed to investigate the photosynthetic characteristics and the synthesis, accumulation, and physicochemical properties of lotus rhizome starches under six SRF levels (CK, S1, S2, S3, S4, and S5).  Compared with CK (0 kg ha–1), the net photosynthetic rate (Pn) and SPAD values of leaves remained at higher levels under SRF treatment.  Further research showed that SRF increased the lotus rhizome yield, the contents of amylose, amylopectin, and total starch, and the number of starch granules.  Among the six SRF levels, S3 (1 035 kg ha–1) showed the greatest difference from CK and produced the highest levels.  With the increasing SRF levels, the peak, hot and final viscosities decreased at first and then increased, but the setback viscosity and pasting temperature increased.  In order to interpret these changes at the molecular level, the activities of key enzymes and relative expression levels of starch accumulation related genes were analyzed.  Each of these parameters also increased under SRF treatment, especially under the S3 treatment.  The results of this study show that SRF, especially S3 (1 035 kg ha–1), is a suitable fertilizer option for lotus planting which can improve lotus rhizome quality by affecting starch accumulations related enzymes and genes.  These results will be useful for SRF application to high-quality lotus rhizome production with low environmental costs.

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Effect of three insect-resistant maizes expressing Cry1Ie, Cry1Ab/Cry2Aj and Cry1Ab on the growth and development of armyworm Mythimna separata (Walker)
SU Hong-hua, JIANG Tao, SUN Yu, GU Hui-jie, WU Jiao-jiao, YANG Yi-zhong
2020, 19 (7): 1842-1849.   DOI: 10.1016/S2095-3119(20)63162-8
Abstract125)      PDF in ScienceDirect      
Three transgenic maize events (IE09S034, Shuangkang 12–5 and C0030.3.5) produced Cry1Ie, Cry1Ab/Cry2Aj and G10-EPSPS, Cry1Ab and EPSPS, respectively, all of which target the Asian corn borer.  The oriental armyworm Mythimna separata (Walker) is the secondary target.  In this study, the effects of the three Bt maizes on the development and survival of armyworm were studied.  The results showed that IE09S034 had insecticidal activity against 1st instar larvae, and the survival rate of armyworm fed with Bt maize for 10 days was 46.2%, significantly lower than that of the control.  The larvae at 3rd–6th instar were more tolerant of the Bt toxin than the early instar larvae.  However, Shuangkang 12-5 had good insecticidal activity against 1st–5th instar larvae.  The mortality was nearly 100% when the larvae were fed with Shuangkang 12-5 before 3rd instar, and the toxin had quick-acting efficacy.  This event significantly inhibited the development of armyworm; that is, the larval duration of the 3rd and 4th instar larvae fed with Shuangkang 12-5 was prolonged by 4.5 and 3.0 days, respectively.  The pupal weight and egg number were also significantly lower than those of the control.  For C0030.3.5, it could control 1st–5th instar larvae effectively.  The mortality rates were all over 50% if 1st–3rd larvae were fed with this event.  The pupal weight of 4th–6th instar larvae fed with Bt maize were only 53.9, 56.8 and 54.6%, respectively, compared to that of the control.  The number of eggs laid was significantly less than the control.  The results indicate that all three transgenic maize events exhibit the potential to provide effective control of early instar larvae of armyworm, which can be commercialized in future to control lepidoptera pests such as Asian corn borer and armyworm.
 
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Cloning and Characterization of a Salt Tolerance-Associated Gene Encoding Trehalose-6-Phosphate Synthase in Sweetpotato
JIANG Tao, ZHAI Hong, WANG Fei-bing, ZHOU Hua-nan, SI Zeng-zhi, HE Shao-zhen , LIU Qing-chang
2014, 13 (8): 1651-1661.   DOI: 10.1016/S2095-3119(13)60534-1
Abstract1466)      PDF in ScienceDirect      
Trehalose plays an important role in metabolic regulation and abiotic stress tolerance in a variety of organisms. In plants, its biosynthesis is catalyzed by two key enzymes: trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP). In the present study, a TPS gene, named IbTPS, was first isolated from sweetpotato (Ipomoea batatas (L.) Lam.) cv. Lushu 3 by rapid amplification of cDNA ends (RACE). The open reading frame (ORF) contained 2 580 nucleotides encoding 859 amino acids with a molecular weight of 97.433 kDa and an isoelectric point (pI) of 5.7. The deduced amino acid sequence showed high identities with TPS of other plants. Real-time quantitative PCR analysis revealed that the expression level of IbTPS gene was significantly higher in stems of Lushu 3 than in its leaves and roots. Subcellular localization analysis in onion epidermal cells indicated that IbTPS gene was located in the nucleus. Transgenic tobacco (cv. Wisconsin 38) plants over-expressing IbTPS gene exhibited significantly higher salt tolerance compared with the control plant. Trehalose and proline content was found to be significantly more accumulated in transgenic tobacco plants than in the wild-type and several stress tolerance related genes were up-regulated. These results suggest that IbTPS gene may enhance salt tolerance of plants by increasing the amount of treahalose and proline and regulating the expression of stress tolerance related genes.
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