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Comprehensive characterization of yam tuber nutrition and medicinal quality of Dioscorea opposita and D. alata from different geographic groups in China
SHAN Nan, WANG Pu-tao, ZHU Qiang-long, SUN Jing-yu, ZHANG Hong-yu, LIU Xing-yue, CAO Tian-xu, CHEN Xin, HUANG Ying-jin, ZHOU Qing-hong
2020, 19 (11): 2839-2848.   DOI: 10.1016/S2095-3119(20)63270-1
Abstract179)      PDF in ScienceDirect      
China is an important domestication center of yams, and two main yam species of Dioscorea opposita and D. alata are commonly cultivated in China.  However, the differences of nutritional and medicinal characteristics between the two species and their subgroups remain unclear, which would greatly affect the resource conservation and commercial utilization of yams.  In this study, typical yam resources including the species of D. opposita (wild and cultivated Ruichang yam from southern China, and Tiegun yam from northern China) and two landraces of D. alata (Longyan yam and Anyuan yam from southern China) were selected as materials.  Nutritional traits and medicinal characteristics were determined and analyzed respectively.  The results showed that there was no significant differences in the content of most nutrients between D. opposita and D. alata, but most cultivated Ruichang yam of D. opposita showed higher levels of starch, soluble sugar, sucrose, and ascorbate in tuber than that in yam from D. alata.  Moreover, an UPLC-MS method was developed for identification and determination of medicinal characteristics in the two species.  The results showed that allantoin can be detected in all selected samples.  Cultivated Ruichang yam of D. opposita possessed the highest allantoin content among the tested materials, and was significantly different with that in Tiegun yam and D. alata. Dioscin was not detected in D. alata. Overall, there was little difference in nutritional composition between D. opposita and D. alata, but the medicinal quality of D. opposita was better than that of D. alata.  Due to the outstanding comprehensive quality, the local variety of cultivated Ruichang yam can be further developed and utilized.
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More children nutrition distribution, less labor incentive: Evidence from Chinese collective agriculture
HUANG Ying-wei, MAO Pei, LI Jun
2019, 18 (10): 2422-2433.   DOI: 10.1016/S2095-3119(19)62733-4
Abstract162)      PDF in ScienceDirect      
Distribution system will affect the labor incentive that has been heatedly discussed by recent literatures. Using a unique micro dataset, this paper demonstrates that the equalitarian distribution system is one of the reasons for the insufficient labor incentive during the Chinese Collective Agriculture period. Specifically speaking, in the distribution of basic rations, the proportion for children (aged 1–3 and 4–7 years) was often beyond their nutrition demand, resulting the dissatisfaction of other families with more laborers and less children, thus these households will reduce their labor supply gradually. At the same time, the existence of outstanding accounts makes it a failure to use work points to buy distributions, which is the mechanism of the distribution system and insufficient labor incentive. All the results have been accepted by the robustness tests. The study will help to understand the distribution system and labor incentive, as well as the failure of the Chinese collective agriculture.
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Overexpression of the Suaeda salsa SsNHX1 gene confers enhanced salt and drought tolerance to transgenic Zea mays
HUANG Ying, ZHANG Xiao-xia, LI Yi-hong, DING Jian-zhou, DU Han-mei, ZHAO Zhuo, ZHOU Li-na, LIU Chan, GAO Shi-bin, CAO Mo-ju, LU Yan-li, ZHANG Su-zhi
2018, 17 (12): 2612-2623.   DOI: 10.1016/S2095-3119(18)61998-7
Abstract300)      PDF in ScienceDirect      
Maize is one of the most important crops worldwide, but it suffers from salt stress when grown in saline-alkaline soil. There is therefore an urgent need to improve maize salt tolerance and crop yield. In this study, the SsNHX1 gene of Suaeda salsa, which encodes a vacuolar membrane Na+/H+ antiporter, was transformed into the maize inbred line 18-599 by Agrobacterium-mediated transformation. Transgenic maize plants overexpressing the SsNHX1 gene showed less growth retardation when treated with an increasing NaCl gradient of up to 1%, indicating enhanced salt tolerance. The improved salt tolerance of transgenic plants was also demonstrated by a significantly elevated seed germination rate (79%) and a reduction in seminal root length inhibition. Moreover, transgenic plants under salt stress exhibited less physiological damage. SsNHX1-overexpressing transgenic maize accumulated more Na+ and K+ than wild-type (WT) plants particularly in the leaves, resulting in a higher ratio of K+/Na+ in the leaves under salt stress. This result revealed that the improved salt tolerance of SsNHX1-overexpressing transgenic maize plants was likely attributed to SsNHX1-mediated localization of Na+ to vacuoles and subsequent maintenance of the cytosolic ionic balance. In addition, SsNHX1 overexpression also improved the drought tolerance of the transgenic maize plants, as rehydrated transgenic plants were restored to normal growth while WT plants did not grow normally after dehydration treatment. Therefore, based on our engineering approach, SsNHX1 represents a promising candidate gene for improving the salt and drought tolerance of maize and other crops.
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Muscle Biological Characteristics of Differentially Expressed Genes in Wujin and Landrace Pigs
XU Hong, HUANG Ying, LI Wei-zhen, YANG Ming-hua, GE Chang-rong, ZHANG Xi, LI Liu-an , GAO Shi-zheng , ZHAO Su-mei
2014, 13 (10): 2236-2242.   DOI: 10.1016/S2095-3119(13)60605-X
Abstract1349)      PDF in ScienceDirect      
The biological chemistry would be responsible for the meat quality. This study tried to investigate the transcript expression profile and explain the characteristics of differentially expressed genes between the Wujin and Landrace pigs. The results showed that 526 differentially expressed genes were found by comparing the transcript expression profile of muscle tissue between Wujin and Landrace pigs. Among them, 335 genes showed up-regulations and 191 genes showed down-regulations in Wujin pigs compared with the Landrace pigs. Gene ontology (GO) analysis indicated that the differentially expressed genes were clustered into three groups involving in protein synthesis, energy metabolism and immune response. Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis found that these differentially expressed genes participated in protein synthesis metabolism, energy metabolism and immune response pathway. The Database for Annotation, Visualization and Integrated Discovery (DAVID) analysis of protein function and protein domains function also confirmed that differentially expressed genes belonged to protein synthesis, energy metabolism and immune response. Genes related protein synthesis metabolism pathway in Landrace was higher than in Wujin pigs. However, differentially expressed genes related energy metabolism and immune response was up-regulated in Wujin pigs compared with Landrace pigs. Quantitative real-time RT-PCR on selected genes was used to confirm the results from the microarray. These suggested that the genes related to protein synthesis, energy metabolism and immune response would contribute to the growth performance, meat quality as well as anti-disease capacity.
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