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Impacts of information about COVID-19 on pig farmers’ production willingness and behavior: Evidence from China

Huan Chen, Lei Mao, Yuehua Zhang
2024, 23 (4): 1429-1441.   DOI: 10.1016/j.jia.2023.11.034
Abstract83)      PDF in ScienceDirect      

This paper examines the impacts of information about COVID-19 on pig farmers’ production willingness by using endorsement experiments and follow-up surveys conducted in 2020 and 2021 in China.  Our results show that, first, farmers were less willing to scale up production when they received information about COVID-19.  The information in 2020 that the second wave of COVID-19 might occur without a vaccine reduced farmers’ willingness to scale up by 13.4%, while the information in 2021 that COVID-19 might continue to spread despite the introduction of vaccine reduced farmers’ willingness by 4.4%.  Second, farmers whose production was affected by COVID-19 were considerably less willing to scale up, given the access to COVID-19 information.  Third, farmers’ production willingness can predict their actual production behavior.

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Karyotype establishment and development of specific molecular markers of Aegilops geniculata Roth based on SLAF-seq 
Yongfu Wang, Jianzhong Fan, Hong Zhang, Pingchuan Deng, Tingdong Li, Chunhuan Chen, Wanquan Ji, Yajuan Wang
2024, 23 (12): 3953-3965.   DOI: 10.1016/j.jia.2023.09.014
Abstract256)      PDF in ScienceDirect      

The constant evolution of pathogens poses a threat to wheat resistance against diseases, endangering food security.  Developing resistant wheat varieties is the most practical approach for circumventing this problem.  As a close relative of wheat, Aegilops geniculata, particularly accession SY159, has evolved numerous beneficial traits that could be applied to improve wheat.  In this study, we established the karyotype of SY159 by fluorescence in situ hybridization (FISH) using the oligonucleotide probes Oligo-pTa535 and Oligo-pSc119.2 and a complete set of wheat–Ae. geniculata accession TA2899 addition lines as a reference.  Using specific-locus amplified fragment sequencing (SLAF-seq) technology, 400 specific markers were established for detecting the SY159 chromosomes with efficiencies reaching 81.5%.  The SY159-specific markers were used to classify the different homologous groups of SY159 against the wheat–Ae. geniculata addition lines.  We used these specific markers on the 7Mg chromosome after classification, and successfully confirmed their suitability for studying the different chromosomes of SY159.  This study provides a foundation for accelerating the application of SY159 in genetic breeding programs designed to improve wheat. 

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Mining elite loci and candidate genes for root morphology-related traits at the seedling stage by genome-wide association studies in upland cotton (Gossypium hirsutum L.) 
Huaxiang Wu, Xiaohui Song, Muhammad Waqas-Amjid, Chuan Chen, Dayong Zhang, Wangzhen Guo
2024, 23 (10): 3406-3418.   DOI: 10.1016/j.jia.2024.03.037
Abstract85)      PDF in ScienceDirect      
Root system architecture plays an essential role in water and nutrient acquisition in plants, and it is significantly involved in plant adaptations to various environmental stresses.  In this study, a panel of 242 cotton accessions was collected to investigate six root morphological traits at the seedling stage, including main root length (MRL), root fresh weight (RFW), total root length (TRL), root surface area (RSA), root volume (RV), and root average diameter (AvgD).  The correlation analysis of the six root morphological traits revealed strong positive correlations of TRL with RSA, as well as RV with RSA and AvgD, whereas a significant negative correlation was found between TRL and AvgD.  Subsequently, a genome-wide association study (GWAS) was performed using the root phenotypic and genotypic data reported previously for the 242 accessions using 56,010 single nucleotide polymorphisms (SNPs) from the CottonSNP80K array.  A total of 41 quantitative trait loci (QTLs) were identified, including nine for MRL, six for RFW, nine for TRL, 12 for RSA, 12 for RV and two for AvgD.  Among them, eight QTLs were repeatedly detected in two or more traits.  Integrating these results with a transcriptome analysis, we identified 17 candidate genes with high transcript values of transcripts per million (TPM)≥30 in the roots.  Furthermore, we functionally verified the candidate gene GH_D05G2106, which encodes a WPP domain protein 2 in root development.  A virus-induced gene silencing (VIGS) assay showed that knocking down GH_D05G2106 significantly inhibited root development in cotton, indicating its positive role in root system architecture formation.  Collectively, these results provide a theoretical basis and candidate genes for future studies on cotton root developmental biology and root-related cotton breeding.


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Melatonin treatment alleviates chilling injury in mango fruit 'Keitt' by modulating proline metabolism under chilling stress
Mariama KEBBEH, DONG Jing-xian, HUAN Chen, SHEN Shu-ling, LIU Yan, ZHENG Xiao-lin
2023, 22 (3): 935-944.   DOI: 10.1016/j.jia.2023.02.008
Abstract283)      PDF in ScienceDirect      

Mangoes often suffer from low temperature-induced chilling injury (CI) during postharvest cold storage.  Therefore, advanced techniques are crucial and in high demand to solve the chilling stress of mango fruit for a higher value.  This study addresses chilling stress modulation by investigating the effects of melatonin treatment on CI, proline metabolism, and related gene expressions of ‘Keitt’ mango during cold storage after dipped in 0 (control), 0.1 (MT1), and 0.2 mmol L–1 (MT2) melatonin solution for 30 min.  The results revealed that melatonin treatment in MT1 significantly reduced CI development and increased proline content in mango fruit during cold storage compared to the control.  These changes were along with increases in the activity of critical enzymes as well as the expression of encoding genes involved in proline biosynthesis, such as pyrroline-5-carboxylate synthetase (P5CS), pyrroline-5-carboxylate reductase (P5CR), ornithine D-aminotransferase (OAT), P5CS2, P5CR2, and OAT3.  Additionally, proline dehydrogenase (PDH) activity and the expression of the PDH3 gene associated with proline dehydrogenation were lower in MT1-treated mangoes than the controlled group.  Thus, melatonin treatment has regulated proline metabolism resulting in the accumulation of proline, subsequently contributing to enhancing the chilling tolerance of ‘Keitt’ mango fruit.

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PDL1-dependent trans-acting siRNAs regulate lateral organ polarity development in rice
Yi Zhang, Jing You, Jun Tang, Wenwen Xiao, Mi Wei, Ruhui Wu, Jinyan Liu, Hanying Zong, Shuoyu Zhang, Jie Qiu, Huan Chen, Yinghua Ling, Fangming Zhao, Yunfeng Li, Guanghua He, Ting Zhang
DOI: 10.1016/j.jia.2024.01.025 Online: 05 March 2024
Abstract35)      PDF in ScienceDirect      
Leaves and glumes act as lateral organs and have essential effects on photosynthesis and seed morphology, thus affecting yield.  However, the molecular mechanisms controlling their polarity development in rice is still worth further analysis.  Here, we isolated a polarity defect of lateral organs 1 (pdl1) mutant in rice, which exhibits twisted/filamentous-shaped leaves and cracked/filamentous-shaped lemmas caused by defects in polarity development.  PDL1 encodes a SUPPRESSOR OF GENE SILENCING 3 protein localized in the cytoplasm granules.  PDL1 is expressed in the shoot apical meristem, inflorescence meristem, floral meristem, and lateral organs including leaf and floral organs.  PDL1 is involved in the synthesis of tasiR-ARF, which may subsequently modulate the expression of OsARFs.  Meanwhile, the expression of abaxial miR165/166 and the adaxial identity genes OSHBs was increased and decreased significantly, respectively.  The results of this study clarified the molecular mechanism that the PDL1-mediated tasiR-ARF synthesis regulates the lateral organ polarity development in rice.
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Cytogenetic characterization and molecular marker development of a novel wheat-Thinopyrum ponticum 5E (5D) disomic substitution line with resistance to powdery mildew and stripe rust 
Xiaofang Cheng, Yi Xiao, Luhui Wang, Xiaoying Yang, Pingchuan Deng, Jixin Zhao, Changyou Wang, Chunhuan Chen, Tingdong Li, Wanquan Ji
DOI: 10.1016/j.jia.2024.04.012 Online: 10 May 2024
Abstract30)      PDF in ScienceDirect      
Thinopyrum ponticum (2n=10x=70), a wild relative of common wheat (Triticum aestivum L.), is considered an invaluable genetic resource for wheat improvement due to its abundance of genes that confer resistance to biotic and abiotic stresses.  This study focused on the CH97 line, derived from the BC1F7 progeny of a cross between wheat cv. 7182 and Th. ponticum.  Cytological evidence showed that CH97 has 42 chromosomes, forming 21 bivalents at meiotic metaphase I, with the bivalents subsequently separating and moving to opposite poles during meiotic anaphase I.  Through a combination of FISH (fluorescence in situ hybridization), GISH (genomic in situ hybridization), mc-GISH (multicolor genomic in situ hybridization), and liquid array analysis, it was determined that CH97 comprises 40 wheat chromosomes and two alien chromosomes from the Ee genome of Th. ponticum, featuring the absence of a pair of 5D chromosomes and variations in 1B, 6B, and 7B chromosomes.  These findings confirm that CH97 is a stable wheat-Th. ponticum 5E (5D) alien disomic substitution line.  Inoculation experiments revealed that CH97 exhibits high resistance to wheat powdery mildew and stripe rust throughout the growth period, in contrast to the highly susceptible common wheat parent 7182.  Compared to 7182, CH97 displayed improvements in spikelets per spike, thousand-kernel weight, and kernel length.  Additionally, utilizing SLAF-seq technology, chromosome 5E-specific molecular markers were developed and validated, achieving a 33.3% success rate, which facilitates marker-assisted selection to enhance disease resistance in wheat.  Overall, the CH97 substitution line, with its resistance to diseases and improved agronomic traits represents valuable new germplasm for wheat chromosome engineering and breeding.
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