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Recent advances in immunocastration in sheep and goat and its animal welfare benefits: A review
ZENG Fan-mei, DING Yi, Teketay WASSIE, JING Hai-jing, Sohail AHMED, LIU Gui-qiong, JIANG Xun-ping
2022, 21 (2): 299-309.   DOI: 10.1016/S2095-3119(21)63670-5
Abstract295)      PDF in ScienceDirect      
Castration of male animals is a common practice in the meat industry aimed at reducing aggressive behavior, preventing unpleasant flavor, and controlling undesirable breeding.  For many years, mechanical castration and surgical castration have been practiced to sterilize the animals.  However, these castration methods are not humane because of the associated risk of death, pain, and stress.  Recently, immunocastration targeting the hypothalamic-pituitary-gonadal axis (HPG) axis has been reported as an animal-friendly approach that circumvents many of the concerns with conventional castration, and suggested by researches as an alternative to surgical castration.  However, there is no compilation of updated information on the use of immunocastration in sheep and goats.  Therefore, this review aims to summarize the developmental process from traditional surgical castration to immunocastration and to screen the process of immune targets.  It also compares the respective advantages and disadvantages of traditional castration technologies and immunocastration, in particular including analyses in the characteristics, features application and welfare benefits of immunocastration in sheep and goats were also analyzed.
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The CRISPR/Cas9 induces large genomic fragment deletions of MSTN and phenotypic changes in sheep
DING Yi, ZHOU Shi-wei, DING Qiang, CAI Bei, ZHAO Xiao-e, ZHONG Shu, JIN Miao-han, WANG Xiao-long, MA Bao-hua, CHEN Yu-lin
2020, 19 (4): 1065-1073.   DOI: 10.1016/S2095-3119(19)62853-4
Abstract230)      PDF in ScienceDirect      
The CRISPR/Cas9 system has been extensively used to engineer genetic loci for the generation of knockouts, insertions, and point mutations in animal models.  However, many mutations that have been reported in animals are small insertions or deletions.  This study used the CRISPR/Cas9 system to induce large DNA fragment deletions in MSTN via three guide RNAs in sheep.  This successfully achieved the precise gene editing of the ovine MSTN gene by injecting both Cas9 mRNA and sgRNAs into embryos at the one-cell stage.  Of 10 edited animals, 3 animals (30%) exhibited large genomic fragment deletions (~5 kb).  Furthermore, the body weights of these 3 animals were significantly different (P0<0.0001, P15=0.001, P30=0.005, P60=0.027) between lambs with large deletions and wildtype lambs.  In addition, the edited lambs were also significantly different (P0<0.0001, P15<0.0001, P30=0.002, P60=0.011) compared with wildtype.  These results suggest that the generated MSTN knockout sheep is a reliable and effective animal model for further study.  Furthermore, this method is time- and labor-saving, and efficient for the creation of animal models for agriculture, biology, and medicine.
 
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Antibacterial Effect of Cinnamon Oil Combined with Thyme or Clove Oil
LU Fei , DING Yi-cheng , YE Xing-qian , DING Yu-ting
2011, 10 (9): 1482-1487.   DOI: 10.1016/S1671-2927(11)60142-9
Abstract3662)      PDF in ScienceDirect      
Antibacterial effect of cinnamon oil combined with thyme or clove oil was studied in this paper. Agar dilution method wasused to determine the minimum inhibitory concentrations (MICs) of the essential oils (EOs) of cinnamon, thyme, and cloveoil against three Gram-positive bacteria (Bacillus subtilis, Bacillus cereus, and Staphylococcus aureus), and two Gramnegativebacteria (Escherichia coli and Salmonella typhimurium). The results showed that cinnamon was a promisingantibacterial substance with MIC ranged from 0.1 to 0.4 μL mL-1 for the five bacterial species. Agar dilution checkerboardmethod was used to test the combined antibacterial effect of cinnamon oil with thyme or clove oil. Combination ofcinnamon and thyme oil showed an additive effect against all selected bacteria, and combination of cinnamon and cloveoil displayed an additive effect against B. subtilis, B. cereus, S. aureus, and an indifferent effect against E. coli andS. typhimurium. Furthermore, gas chromatography-mass spectrometry (GC-MS) measurement was used to analyze thecomponents of the EOs, and the main components of cinnamon, thyme, and clove were cinnamaldehyde, thymol, carvacroland p-cymene, and eugenol, respectively.
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