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1. 一个编码B-BOX蛋白的GhDR基因上的2bp移码缺失与陆地棉矮杆红叶性状共分离
WANG Xue-feng, SHAO Dong-nan, LIANG Qian, FENG Xiao-kang, ZHU Qian-hao, YANG Yong-lin, LIU Feng, ZHANG Xin-yu, LI Yan-jun, SUN Jie, XUE Fei
Journal of Integrative Agriculture    2023, 22 (7): 2000-2014.   DOI: 10.1016/j.jia.2022.10.007
摘要291)      PDF    收藏

株型和叶色是棉花纤维产量的重要影响因素。本研究基于遗传分析、茎秆石蜡切片和植物激素处理方法,发现棉花矮红突变体DR一个赤霉素敏感型突变体,由一个单显性基因位点突变引起,将其命名为GhDR。通过BSA-seq结合靶向测序基因型检测GBTS方法控制突变性状基因定位A09 染色体约197 kb候选区间包含 25 个注释基因。基于候选基因的注释信息及其在突变体和正常植株之间的序列和表达差异GH_A09G2280基因被认为是控制矮红突变体表型的最佳候选基因。在DR突变体GhDR/GH_A09G2280基因编码区发现一个2 bp缺失,导致GhDR基因产生移码突变,蛋白翻译提前终止GhDR是拟南芥AtBBX24的同源基因,编码B-box锌指蛋白。移码缺失导致GhDR C末端缺失核定位结构域和VP结构,并改变了其亚细胞定位结果比较转录组分析表明,在DR突变体中,参与赤霉素生物合成和信号转导的关键基因下调表达,而与赤霉素降解和花青素生物合成相关基因上调表达。研究初步揭示了GhDR基因同时调控棉花株型和花青素积累的潜在分子机制

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2. Does poverty-alleviation-based industry development improve farmers’ livelihood capital?
LIU Ming-yue, FENG Xiao-long, WANG San-gui, ZHONG Yu
Journal of Integrative Agriculture    2021, 20 (4): 915-926.   DOI: 10.1016/S2095-3119(20)63449-9
摘要206)      PDF    收藏

精准扶贫是中国全面建设小康社会、实现中华民族伟大“中国梦”的重要保障。产业扶贫是精准扶贫“五个一批”工程的重头戏,也是其他扶贫措施取得实效的重要基础。本文基于可持续生计框架,采用综合评价方法测度农户的生计资本,进而利用PSM-DID方法估计产业扶贫对农户生计资本的影响效应。结果显示,产业扶贫对农户生计资本有显著的正向影响,能显著提升农户的人力资本、社会资本和金融资本,但对自然资本和物质资本的影响不显著。产业扶贫对农户生计资本的影响具有异质性,对非贫困户的影响显著于贫困户。另外,产业扶贫对农户生计资本的影响也存在地区差异,对贵州农户的影响大于四川,但对甘肃农户的影响不显著。针对以上结论,未来需要通过加大人力资本培育力度、增强金融资本创新手段、发挥社会资本引导作用等提高农户的可持续生计,同时更要加大对贫困户的产业扶持力度。


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3. Income effects of poverty alleviation relocation program on rural farmers in China
LENG Gan-xiao, FENG Xiao-long, QIU Huan-guang
Journal of Integrative Agriculture    2021, 20 (4): 891-904.   DOI: 10.1016/S2095-3119(20)63583-3
摘要150)      PDF    收藏

文基于8省16县中国易地扶贫搬迁户面板数据,分析了易地扶贫搬迁对农村家庭人均收入的影响,并评估各种搬迁方式的异质性增收效应。结果表明,易地扶贫搬迁可以增加搬迁人口的总收入。城镇安置的搬迁户工资性收入有显著增加,村内安置的搬迁户的农业收入有显著增加。进一步的分析表明,村内安置的搬迁户的收入增加主要得益于农业技术培训的发展,而城镇安置的搬迁户的收入增加主要是由于医疗等公共服务保障的改善。因此,对于村内安置的搬迁户,应加强当地的产业发展和农业技术培训。对于城镇安置的搬迁户,应通过提高安置区的非农就业服务和公共保障服务使其长期稳定发展。


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4. Viricidal activity of several disinfectants against African swine fever virus
JIANG Cheng-gang, SUN Ying, ZHANG Fan, AI Xin, FENG Xiao-ning, HU Wei, ZHANG Xian-feng, ZHAO Dong-ming, BU Zhi-gao, HE Xi-jun
Journal of Integrative Agriculture    2021, 20 (11): 3084-3088.   DOI: 10.1016/S2095-3119(21)63631-6
摘要114)      PDF    收藏

本研究以表达eGFP重组非洲猪瘟病毒为指示病毒,通过体外细胞培养方法,模拟浸泡和喷雾方式,在室温条件下,观察消毒剂不同浓度、不同时间作用后是否产生可见荧光信号为标准,来判定病毒是否完全被消毒剂灭活。结果显示,模拟浸泡消毒方式,在室温条件下,碘酸混合溶液0.5%浓度,作用10分钟能完全灭活非洲猪瘟病毒;过硫酸氢钾复合粉0.25%浓度作用30分钟能完全灭活非洲猪瘟病毒;枸橼酸粉0.25%浓度作用30分钟能完全灭活非洲猪瘟病毒;二氯异氰脲酸钠粉0.125%浓度作用60分钟能完全灭活非洲猪瘟病毒;戊二醛癸甲溴铵溶液0.2%浓度作用60分钟能完全灭活非洲猪瘟病毒;癸甲溴铵溶液0.5%浓度作用60分钟能完全灭活非洲猪瘟病毒。模拟喷雾消毒方式,在室温条件下,碘酸混合溶液0.5%浓度,作用10分钟能完全灭活非洲猪瘟病毒;过硫酸氢钾复合粉0.25%浓度作用60分钟能完全灭活非洲猪瘟病毒;枸橼酸粉0.5%浓度作用30分钟能完全灭活非洲猪瘟病毒;二氯异氰脲酸钠粉0.25%浓度作用60分钟能完全灭活非洲猪瘟病毒;戊二醛癸甲溴铵溶液0.2%浓度作用60分钟能完全灭活非洲猪瘟病毒;癸甲溴铵溶液0.5%浓度作用60分钟能完全灭活非洲猪瘟病毒。这些结果表明,常用市售6种消毒剂,在实验室条件下,不同浓度和作用时间均能有效灭活非洲猪瘟病毒,本实验建立了一种应用表达eGFP重组非洲猪瘟病毒为指示病毒,快速准确评价消毒剂灭活非洲猪瘟病毒效果的方法,为非洲猪瘟的生物安全防控提供有力理论依据和技术支持。


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5. Genetic effects and plant architecture influences on outcrossing rate in soybean
YAN Hao, ZHANG Jing-yong, ZHANG Chun-bao, PENG Bao, ZHANG Wei-long, WANG Peng-nian, DING Xiao-yang, LIU Bao-hui, FENG Xian-zhong, ZHAO Li-mei
Journal of Integrative Agriculture    2019, 18 (9): 1971-1979.   DOI: 10.1016/S2095-3119(18)62054-4
摘要168)      PDF    收藏
Outcrossing rate is an important determinant of cytoplasmic male sterile (CMS) breeding and hybrid seed production for heterosis in soybean.  Parental lines with a high outcrossing rate were screened for backcross breeding to obtain the high outcrossing rate maintenance B-lines and sterile A-lines.  Application in production practices will help to increase hybrid soybean production.  In this study, JLCMS82B and JLCMS89B were selected as parents for the construction of outcrossing rate segregation populations, and the progeny-array approach (PAA) and glyphosate resistant gene markers were used to determine outcrossing rates.  We found that: (1) The outcrossing rate between JLCMS82B and JLCMS89B was significantly different; (2) the outcrossing rate of the F2 segregating populations was a quantitative trait, though whether an additive or epistatic effect exists required analysis with a triple test intersection analysis; (3) agronomic traits correlated with outcrossing rate; outcrossing rate was the highest with plant height of about 84 cm, lower number of plant branches, earlier flowering time, larger angle between the branches and the main stem, and with more divergent plant morphology.  Correlation analysis between agronomic traits and outcrossing rate can effectively guide the screening of parents with a high outcrossing rate.
 
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6. How plant density affects maize spike differentiation, kernel set, and grain yield formation in Northeast China?
ZHANG Ming, CHEN Tao, Hojatollah Latifmanesh, FENG Xiao-min, CAO Tie-hua, QIAN Chun-rong, DENG Ai-xing, SONG Zhen-wei, ZHANG Wei-jian
Journal of Integrative Agriculture    2018, 17 (08): 1745-1757.   DOI: 10.1016/S2095-3119(17)61877-X
摘要508)      PDF    收藏
Received  14 August, 2017    Accepted  20 December, 2017
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7. Characterization of dwarf mutants and molecular mapping of a dwarf locus in soybean
CHENG Wen, GAO Jin-shan, FENG Xing-xing, SHAO Qun, YANG Su-xin, FENG Xian-zhong
Journal of Integrative Agriculture    2016, 15 (10): 2228-2236.   DOI: 10.1016/S2095-3119(15)61312-0
摘要1775)      PDF    收藏
    Plant height is one of the most important traits in soybean. The semi-dwarf soybean cultivars could improve the ability of lodging resistance to obtain higher yield. To broaden the dwarfism germplasm resources in soybean, 44 dwarf mutants were identified from a gamma rays mutagenized M2 population. Two of these mutants, Gmdwf1 (Glycine max dwarf 1) and Gmdwf2 (Glycine max dwarf 2), were investigated in this study. Genetic analysis showed that both mutants were inherited in a recessive manner and their mutated regions were delimited to a 2.610-Mb region on chromosome 1 by preliminary mapping. Further fine mapping study proved that the two mutants had a common deletion region of 1.552 Mb in the target region, which was located in a novel locus site without being reported previously. The dwarfism of Gmdwf1 could not be rescued by gibberellin (GA) and brassinolide (BR) treatments, which indicated that the biosynthesis of these hormones was not deficient in Gmdwf1.
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8. cDNA cloning and characterization of the carboxylesterase pxCCE016b from the diamondback moth, Plutella xylostella L.
HU Zhen-di, FENG Xia, LIN Qing-sheng, CHEN Huan-yu, LI Zhen-yu, YIN Fei, LIANG Pei, GAO Xi-wu
Journal of Integrative Agriculture    2016, 15 (05): 1059-1068.   DOI: 10.1016/S2095-3119(15)61278-3
摘要1668)      PDF    收藏
    Carboxylesterase is a multifunctional superfamily and can be found in almost all living organisms. As the metabolic enzymes, carboxylesterases are involved in insecticides resistance in insects for long time. In our previous studies, the enhanced carboxylesterase activities were found in the chlorantraniliprole resistance strain of diamondback moth (DBM). However, the related enzyme gene of chlorantraniliprole resistance has not been clear in this strain. Here, a full-length cDNA of carboxylesterase pxCCE016b was cloned and exogenously expressed in Escherichia coli at the first time, which contained a 1 693 bp open reading frame (ORF) and encoded a protein of 542 amino acids. Sequence analysis showed that this cDNA has a predicted mass of 61.56 kDa and a theoretical isoelectric point value of 5.78. The sequence of deduced amino acid possessed the classical structural features: a type-B carboxylesterase signature 2 (EDCLYLNVYTK), a type-B carboxylesterase serine active site (FGGDPENITIFGESAG) and the catalytic triad (Ser186, Glu316, and His444). The real-time quantitative PCR (qPCR) analysis showed that the expression level of the pxCCE016b was significantly higher in the chlorantraniliprole resistant strain than in the susceptible strain. Furthermore, pxCCE016b was highly expressed in the midgut and epidermis of the DBM larvae. When the 3rd-instar larvae of resistant DBM were exposed to abamectin, alpha-cypermethrin, chlorantraniliprole, spinosad, chlorfenapyr and indoxacarb insecticides, the up-regulated expression of pxCCE016b was observed only in the group treated by chlorantraniliprole. In addition, recombinant vector pET-pxCCE016b was constructed with the most coding region (1 293 bp) and large number of soluble recombinant proteins (less than 48 kDa) were expressed successfully with prokaryotic cell. Western blot analysis showed that it was coded by pxCCE016b. All the above findings provide important information for further functional study, although we are uncertainty whether the pxCCE016b gene is actually involved in chlorantraniliprole resistance.
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9. Biochemical Mechanism of Chlorantraniliprole Resistance in the Diamondback Moth, Plutella xylostella Linnaeus
HU Zhen-di, FENG Xia, LIN Qing-sheng, CHEN Huan-yu, LI Zhen-yu, YIN Fei, LIANG Pei , GAO Xi-wu
Journal of Integrative Agriculture    2014, 13 (11): 2452-2459.   DOI: 10.1016/S2095-3119(14)60748-6
摘要1337)      PDF    收藏
The insecticide chlorantraniliprole exhibits good efficacy and plays an important role in controlling the diamondback moth, Plutella xylostella Linnaeus. However, resistance to chlorantraniliprole has been observed recently in some field populations. At present study, diamondback moths with resistance to chlorantraniliprole (resistant ratio (RR) was 82.18) for biochemical assays were selected. The assays were performed to determine potential resistance mechanisms. The results showed that the selected resistant moths (GDLZ-R) and susceptible moth could be synergized by known metabolic inhibitors such as piperonyl butoxide (PBO), triphenyl phosphate (TPP) and diethyl-maleate (DEM) at different levels (1.68-5.50-fold and 2.20-2.89-fold, respectively), and DEM showed the maximum synergism in both strains. In enzymes assays, a high level of glutathione-S-transferase (GST) was observed in the resistant moth, in contrast, moths that are susceptible to the insecticide had only 1/3 the GST activity of the resistant moths. The analysis of short-term exposure of chlorantraniliprole on biochemical response in the resistant strain also showed that GST activity was significantly elevated after exposure to a sub-lethal concentration of chlorantraniliprole (about 1/3 LC50, 12 mg L-1) 12 and 24 h, respectively. The results show that there is a strong correlation between the enzyme activity and resistance, and GST is likely the main detoxification mechanism responsible for resistance to chlorantraniliprole in P. xylostella L., cytochrome P450 monooxygenase (P450) and carboxy-lesterase (CarE) are involved in to some extent.
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10. 菌群YM2的富集、驯化、降解、适应机制以及烟嘧磺隆的生物修复
Yufeng Xiao, Meiqi Dong, Xian Wu, Shuang Liang, Ranhong Li, Hongyu Pan, Hao Zhang
Journal of Integrative Agriculture    DOI: 10.1016/j.jia.2024.03.004
录用日期: 2024-03-21