Characterization of dwarf mutants and molecular mapping of a dwarf locus in soybean

doi:10.1016/S2095-3119(15)61312-0

Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (10): 2228-2236.DOI: 10.1016/S2095-3119(15)61312-0

收稿日期:2015-10-08 出版日期:2016-10-01 发布日期:2016-10-01

Characterization of dwarf mutants and molecular mapping of a dwarf locus in soybean

CHENG Wen¹, GAO Jin-shan², FENG Xing-xing², SHAO Qun¹, YANG Su-xin², FENG Xian-zhong^{1, 2}

¹ Key Laboratory of Systems Biology in Universities of Shandong/College of Life Science, Shandong Normal University, Jinan 250014, P.R.China
² Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, P.R.China

Received:2015-10-08 Online:2016-10-01 Published:2016-10-01
Contact: FENG Xian-zhong, Tel: +86-431-85655051, Fax: +86-431-85542298, E-mail: fengxianzhong@iga.ac.cn
Supported by:
This study was supported by the National Natural Science Foundation of China (31171571 and 31571692) and the One Hundred Person Project of the Chinese Academy of Sciences.

摘要/Abstract

Abstract: Plant height is one of the most important traits in soybean. The semi-dwarf soybean cultivars could improve the ability of lodging resistance to obtain higher yield. To broaden the dwarfism germplasm resources in soybean, 44 dwarf mutants were identified from a gamma rays mutagenized M2 population. Two of these mutants, Gmdwf1 (Glycine max dwarf 1) and Gmdwf2 (Glycine max dwarf 2), were investigated in this study. Genetic analysis showed that both mutants were inherited in a recessive manner and their mutated regions were delimited to a 2.610-Mb region on chromosome 1 by preliminary mapping. Further fine mapping study proved that the two mutants had a common deletion region of 1.552 Mb in the target region, which was located in a novel locus site without being reported previously. The dwarfism of Gmdwf1 could not be rescued by gibberellin (GA) and brassinolide (BR) treatments, which indicated that the biosynthesis of these hormones was not deficient in Gmdwf1.

Key words: soybean , dwarf mutant , mapping , BR , GA

CHENG Wen, GAO Jin-shan, FENG Xing-xing, SHAO Qun, YANG Su-xin, FENG Xian-zhong. [J]. Journal of Integrative Agriculture, 2016, 15(10): 2228-2236.

CHENG Wen, GAO Jin-shan, FENG Xing-xing, SHAO Qun, YANG Su-xin, FENG Xian-zhong. Characterization of dwarf mutants and molecular mapping of a dwarf locus in soybean[J]. Journal of Integrative Agriculture, 2016, 15(10): 2228-2236.

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Characterization of dwarf mutants and molecular mapping of a dwarf locus in soybean

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