一个编码B-BOX蛋白的GhDR基因上的2bp移码缺失与陆地棉矮杆红叶性状共分离

doi:10.1016/j.jia.2022.10.007

摘要/Abstract

摘要：

株型和叶色是棉花纤维产量的重要影响因素。本研究基于遗传分析、茎秆石蜡切片和植物激素处理方法，发现棉花矮红株突变体（DR）是一个赤霉素敏感型突变体，由一个单显性基因位点突变引起，将其命名为GhDR。通过BSA-seq结合靶向测序基因型检测（GBTS）方法将控制突变性状基因定位在A09 染色体约197 kb的候选区间内，包含 25 个注释基因。基于候选基因的注释信息，及其在突变体和正常植株之间的序列和表达差异，GH_A09G2280基因被认为是控制矮红突变体表型的最佳候选基因。在DR突变体GhDR/GH_A09G2280基因编码区发现了一个2 bp的缺失，导致GhDR基因产生移码突变，蛋白翻译提前终止。GhDR是拟南芥AtBBX24的同源基因，编码B-box锌指蛋白。移码缺失导致GhDR 的C末端缺失了核定位结构域和VP结构域，并改变了其亚细胞定位结果。比较转录组分析表明，在DR突变体中，参与赤霉素生物合成和信号转导的关键基因下调表达，而与赤霉素降解和花青素生物合成相关基因上调表达。本研究初步揭示了GhDR基因同时调控棉花株型和花青素积累的潜在分子机制。

Abstract: Plant architecture and leaf color are important factors influencing cotton fiber yield. In this study, based on genetic analysis, stem paraffin sectioning, and phytohormone treatments, we showed that the dwarf-red (DR) cotton mutant is a gibberellin-sensitive mutant caused by a mutation in a single dominant locus, designated GhDR. Using bulked segregant analysis (BSA) and genotyping by target sequencing (GBTS) approaches, we located the causative mutation to a ~197-kb genetic interval on chromosome A09 containing 25 annotated genes. Based on gene annotation and expression changes between the mutant and normal plants, GH_A09G2280 was considered to be the best candidate gene responsible for the dwarf and red mutant phenotypes. A 2-nucleotide deletion was found in the coding region of GhDR/GH_A09G2280 in the DR mutant, which caused a frameshift and truncation of GhDR. GhDR is a homolog of Arabidopsis AtBBX24, and encodes a B-box zinc finger protein. The frameshift deletion eliminated the C-terminal nuclear localization domain and the VP domain of GhDR, and altered its subcellular localization. A comparative transcriptome analysis demonstrated downregulation of the key genes involved in gibberellin biosynthesis and the signaling transduction network, as well as upregulation of the genes related to gibberellin degradation and the anthocyanin biosynthetic pathway in the DR mutant. The results of this study revealed the potential molecular basis by which plant architecture and anthocyanin accumulation are regulated in cotton.

Key words: cotton , BBX , dwarf , anthocyanin , gibberellin

WANG Xue-feng, SHAO Dong-nan, LIANG Qian, FENG Xiao-kang, ZHU Qian-hao, YANG Yong-lin, LIU Feng, ZHANG Xin-yu, LI Yan-jun, SUN Jie, XUE Fei. 一个编码B-BOX蛋白的GhDR基因上的2bp移码缺失与陆地棉矮杆红叶性状共分离[J]. Journal of Integrative Agriculture, 2023, 22(7): 2000-2014.

WANG Xue-feng, SHAO Dong-nan, LIANG Qian, FENG Xiao-kang, ZHU Qian-hao, YANG Yong-lin, LIU Feng, ZHANG Xin-yu, LI Yan-jun, SUN Jie, XUE Fei. A 2-bp frameshift deletion at GhDR, which encodes a B-BOX protein that co-segregates with the dwarf-red phenotype in Gossypium hirsutum L.[J]. Journal of Integrative Agriculture, 2023, 22(7): 2000-2014.

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