Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (12): 2618-2625.DOI: 10.1016/S2095-3119(14)60933-3

• 论文 • 上一篇    

Analysis of the function of D279N mutation of VP2 of infectious bursal disease virus

 QI Xiao-le, LU Zhen, WANG Nian, CHEN Yu-ming, ZHANG Li-zhou, GAO Li, LI Kai, REN Xian-gang, WANG Yong-qiang, GAO Hong-lei, GAO Yu-long, Nicolas Eterradossi, WANG Xiao-mei   

  1. 1、Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute,
    Chinese Academy of Agricultural Sciences, Harbin 150001, P.R.China
    2、Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou
    225009, P.R.China
    3、Avian and Rabbit Virology, Immunology and Parasitology Unit, OIE Reference Laboratory for Infectious Bursal Disease, French
    Agency for Food, Environmental and Occupational Health Safety (Anses), Ploufragan 22440, France
  • 收稿日期:2014-10-10 出版日期:2015-12-01 发布日期:2015-12-16
  • 通讯作者: WANG Xiao-mei, Tel: +86-18946066004,Fax: +86-451-51997166, E-mail: xmw@hvri.ac.cn
  • 作者简介:QI Xiao-le, Tel: +86-18946066078, E-mail: qxl@hvri.ac.cn;
  • 基金资助:

    This study was supported by a grant from National Natural Science Foundation of China (31430087), the Scientific and Technological Research Project of Harbin, China (2014AB3AN058), the Special Fund for Scientific and Technological Innovative Talents of Harbin, China (2014RFQYJ129), China-France Cai-Yuanpei Program (2011008007), the Modern Agro-industry Technology Research System of China (nycytx-42-G3-01).

Analysis of the function of D279N mutation of VP2 of infectious bursal disease virus

 QI Xiao-le, LU Zhen, WANG Nian, CHEN Yu-ming, ZHANG Li-zhou, GAO Li, LI Kai, REN Xian-gang, WANG Yong-qiang, GAO Hong-lei, GAO Yu-long, Nicolas Eterradossi, WANG Xiao-mei   

  1. 1、Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute,
    Chinese Academy of Agricultural Sciences, Harbin 150001, P.R.China
    2、Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou
    225009, P.R.China
    3、Avian and Rabbit Virology, Immunology and Parasitology Unit, OIE Reference Laboratory for Infectious Bursal Disease, French
    Agency for Food, Environmental and Occupational Health Safety (Anses), Ploufragan 22440, France
  • Received:2014-10-10 Online:2015-12-01 Published:2015-12-16
  • Contact: WANG Xiao-mei, Tel: +86-18946066004,Fax: +86-451-51997166, E-mail: xmw@hvri.ac.cn
  • About author:QI Xiao-le, Tel: +86-18946066078, E-mail: qxl@hvri.ac.cn;
  • Supported by:

    This study was supported by a grant from National Natural Science Foundation of China (31430087), the Scientific and Technological Research Project of Harbin, China (2014AB3AN058), the Special Fund for Scientific and Technological Innovative Talents of Harbin, China (2014RFQYJ129), China-France Cai-Yuanpei Program (2011008007), the Modern Agro-industry Technology Research System of China (nycytx-42-G3-01).

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摘要： Infectious bursal disease virus (IBDV) is responsible for the highly contagious infectious bursal disease of chickens. Further understanding the gene-function is necessary to design the tailored vaccine. The amino acid residue 279, located on strand PF of VP2, is one of the three residues that have been reported to be involved in cell-tropism but with some inconsistency. In this study, to further clarify the amino acids involved in the cell tropism of IBDV, a series of mutations about residue 279 were introduced into the VP2 of vvIBDV Gx strain. With the reverse genetic system, we found single mutation of D279N, double mutations of D279N/A284T or Q253H/D279N were not enough to adapt IBDV to chicken embryo fibroblast (CEF) cell. To evaluate whether residue 279 could influence the replication and virulence of IBDV, the virus rGxHT-279 with three mutations (Q253H/D279N/A284T) was rescued and evaluated. Results showed that the mutation of residue 279 in VP2 had no efficient effects on both the replication efficiency in vitro and the virulence to SPF chickens of IBDV. In summary, the results demonstrated that residue 279 of VP2 did not contribute efficiently to cell tropism, replication efficiency, and virulence of IBDV at least in some strains. These findings provided further information for understanding the gene function of IBDV.

关键词: infectious bursal disease virus (IBDV) , residue 279 , cell tropism , virulence

Abstract: Infectious bursal disease virus (IBDV) is responsible for the highly contagious infectious bursal disease of chickens. Further understanding the gene-function is necessary to design the tailored vaccine. The amino acid residue 279, located on strand PF of VP2, is one of the three residues that have been reported to be involved in cell-tropism but with some inconsistency. In this study, to further clarify the amino acids involved in the cell tropism of IBDV, a series of mutations about residue 279 were introduced into the VP2 of vvIBDV Gx strain. With the reverse genetic system, we found single mutation of D279N, double mutations of D279N/A284T or Q253H/D279N were not enough to adapt IBDV to chicken embryo fibroblast (CEF) cell. To evaluate whether residue 279 could influence the replication and virulence of IBDV, the virus rGxHT-279 with three mutations (Q253H/D279N/A284T) was rescued and evaluated. Results showed that the mutation of residue 279 in VP2 had no efficient effects on both the replication efficiency in vitro and the virulence to SPF chickens of IBDV. In summary, the results demonstrated that residue 279 of VP2 did not contribute efficiently to cell tropism, replication efficiency, and virulence of IBDV at least in some strains. These findings provided further information for understanding the gene function of IBDV.

Key words: infectious bursal disease virus (IBDV) , residue 279 , cell tropism , virulence