Virulence and Diversity of Blumeria graminis f. sp. tritici Populations in China

doi:10.1016/S2095-3119(13)60669-3

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (11): 2424-2437.DOI: 10.1016/S2095-3119(13)60669-3

Virulence and Diversity of Blumeria graminis f. sp. tritici Populations in China

ZENG Fan-song, YANG Li-jun, GONG Shuang-jun, SHI Wen-qi, ZHANG Xue-jiang, WANG　Hua , XIANG Li-bo, XUE Min-Feng , YU Da-zhao

1、Laboratory of Integrated Pest Management on Crop in Central China, Ministry of Agriculture/Institute for Plant Protection and Soil Science,
Hubei Academy of Agricultural Sciences, Wuhan 430064, P.R.China
2、College of Life Sciences, Wuhan University, Wuhan 430072, P.R.China

收稿日期:2013-07-03 出版日期:2014-11-06 发布日期:2014-11-14
通讯作者: YU Da-zhao, Tel/Fax: +86-27-87380681, E-mail: dazhaoyu@china.com
作者简介:ZENG Fan-song, E-mail: zengfansong2005@126.com;
基金资助:
This work was supported by the National Basic Research Program of China (2013CB127700) and the Special Fund for Agro-Scientific Research in the Public Interest, China (201303016).

Virulence and Diversity of Blumeria graminis f. sp. tritici Populations in China

ZENG Fan-song, YANG Li-jun, GONG Shuang-jun, SHI Wen-qi, ZHANG Xue-jiang, WANG　Hua , XIANG Li-bo, XUE Min-Feng , YU Da-zhao

1、Laboratory of Integrated Pest Management on Crop in Central China, Ministry of Agriculture/Institute for Plant Protection and Soil Science,
Hubei Academy of Agricultural Sciences, Wuhan 430064, P.R.China
2、College of Life Sciences, Wuhan University, Wuhan 430072, P.R.China

Received:2013-07-03 Online:2014-11-06 Published:2014-11-14
Contact: YU Da-zhao, Tel/Fax: +86-27-87380681, E-mail: dazhaoyu@china.com
About author:ZENG Fan-song, E-mail: zengfansong2005@126.com;
Supported by:
This work was supported by the National Basic Research Program of China (2013CB127700) and the Special Fund for Agro-Scientific Research in the Public Interest, China (201303016).

摘要/Abstract

摘要： Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici, is an important disease in China. To characterize the virulence and diversity of the pathogen, 1082 isolates were obtained from 8 major wheat-growing regions during the spring growing season in 2011. The virulence test was performed by inoculation on detached leaves of 22 differential lines with known Pm genes. Frequencies of virulence on these genotypes ranged from 0 to 97.4%. None of the 1 082 isolates was compatible to Pm21 and less than 20.0% were virulent to the genotype carrying Pm13. In contrast, the virulence frequencies of each population was more than 50.0% to differentials carrying Pm1a, Pm3b, Pm3c, Pm3f, Pm5a, Pm6 and Pm8. In total, 1028 pathotypes were detected, of which 984 were unique. Phenotypic diversity indices revealed a high level of diversity within populations. Genetic distance between different populations correlated significantly with geographical distance (R2=0.494, P 0.001). In addition, isolates from Xinjiang appear to form a separate group. Significant positive or negative associations between alleles at pairs of virulence loci were detected in 57 allele pairs to Pm genes. Virulence and diversity of the 8 populations suggested that varieties with effective resistance gene combinations should be developed at a regional level.

关键词: Blumeria graminis f. sp. tritici , virulence , diversity , wheat , China

Abstract: Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici, is an important disease in China. To characterize the virulence and diversity of the pathogen, 1082 isolates were obtained from 8 major wheat-growing regions during the spring growing season in 2011. The virulence test was performed by inoculation on detached leaves of 22 differential lines with known Pm genes. Frequencies of virulence on these genotypes ranged from 0 to 97.4%. None of the 1 082 isolates was compatible to Pm21 and less than 20.0% were virulent to the genotype carrying Pm13. In contrast, the virulence frequencies of each population was more than 50.0% to differentials carrying Pm1a, Pm3b, Pm3c, Pm3f, Pm5a, Pm6 and Pm8. In total, 1028 pathotypes were detected, of which 984 were unique. Phenotypic diversity indices revealed a high level of diversity within populations. Genetic distance between different populations correlated significantly with geographical distance (R2=0.494, P 0.001). In addition, isolates from Xinjiang appear to form a separate group. Significant positive or negative associations between alleles at pairs of virulence loci were detected in 57 allele pairs to Pm genes. Virulence and diversity of the 8 populations suggested that varieties with effective resistance gene combinations should be developed at a regional level.

Key words: Blumeria graminis f. sp. tritici , virulence , diversity , wheat , China

ZENG Fan-song, YANG Li-jun, GONG Shuang-jun, SHI Wen-qi, ZHANG Xue-jiang, WANG　Hua, XIANG Li-bo, XUE Min-Feng , YU Da-zhao. Virulence and Diversity of Blumeria graminis f. sp. tritici Populations in China[J]. Journal of Integrative Agriculture, 2014, 13(11): 2424-2437.

ZENG Fan-song, YANG Li-jun, GONG Shuang-jun, SHI Wen-qi, ZHANG Xue-jiang, WANG　Hua , XIANG Li-bo, XUE Min-Feng , YU Da-zhao. Virulence and Diversity of Blumeria graminis f. sp. tritici Populations in China[J]. Journal of Integrative Agriculture, 2014, 13(11): 2424-2437.

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Virulence and Diversity of Blumeria graminis f. sp. tritici Populations in China

Virulence and Diversity of Blumeria graminis f. sp. tritici Populations in China

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