A Non-Marker Mutagenesis Strategy to Generate Poly-hrp Gene Mutants in the Rice Pathogen Xanthomonas oryzae pv. oryzicola

doi:10.1016/S1671-2927(11)60104-1

Journal of Integrative Agriculture ›› 2011, Vol. 10 ›› Issue (8): 1139-1150.DOI: 10.1016/S1671-2927(11)60104-1

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A Non-Marker Mutagenesis Strategy to Generate Poly-hrp Gene Mutants in the Rice Pathogen Xanthomonas oryzae pv. oryzicola

School of Agriculture and Biology, Shanghai Jiao Tong University

收稿日期:2010-10-08 出版日期:2011-08-15 发布日期:2011-08-16
通讯作者: Correspondence CHEN Gong-you, Professor, Tel: +86-21-34205873, Fax: +86-21-34205873, E-mail:gyouchen@sjtu.edu.cn
作者简介:ZOU Li-fang, E-mail: zoulifang202018@sjtu.edu.cn

A Non-Marker Mutagenesis Strategy to Generate Poly-hrp Gene Mutants in the Rice Pathogen Xanthomonas oryzae pv. oryzicola

School of Agriculture and Biology, Shanghai Jiao Tong University

Received:2010-10-08 Online:2011-08-15 Published:2011-08-16
Contact: Correspondence CHEN Gong-you, Professor, Tel: +86-21-34205873, Fax: +86-21-34205873, E-mail:gyouchen@sjtu.edu.cn
About author:ZOU Li-fang, E-mail: zoulifang202018@sjtu.edu.cn

摘要/Abstract

摘要： Xanthomonas oryzae pv. oryzicola (Xoc), the critical pathogen causing bacterial leaf streak in rice, possesses a hrp clusterthat is responsible for triggering hypersensitive response (HR) in non-host tobacco and pathogenicity in host rice, and isconsidered to be one of the model pathogens in the rice model plant. Here, we developed a high-throughput mutagenesissystem using a two-step integration mediated by a novel suicide vector pKMS1. It was used to generate single or poly-genemutants of hpa1, hpa2, hrcV, hrpE, hpaB, and hrpF gene for functional analysis. In total, five single, four double, and twotriple hrp gene mutants were constructed. The double and triple hrp gene deletion mutants triggered novel phenotypesin planta. Our data suggest that pKMS1 is a useful tool for non-marker mutagenesis of multiple genes in Xoc.

关键词:

Xanthomonas oryzae pv. oryzicola, suicide vector, knockout mutagenesis, hrp gene

Abstract: Xanthomonas oryzae pv. oryzicola (Xoc), the critical pathogen causing bacterial leaf streak in rice, possesses a hrp clusterthat is responsible for triggering hypersensitive response (HR) in non-host tobacco and pathogenicity in host rice, and isconsidered to be one of the model pathogens in the rice model plant. Here, we developed a high-throughput mutagenesissystem using a two-step integration mediated by a novel suicide vector pKMS1. It was used to generate single or poly-genemutants of hpa1, hpa2, hrcV, hrpE, hpaB, and hrpF gene for functional analysis. In total, five single, four double, and twotriple hrp gene mutants were constructed. The double and triple hrp gene deletion mutants triggered novel phenotypesin planta. Our data suggest that pKMS1 is a useful tool for non-marker mutagenesis of multiple genes in Xoc.

Key words:

Xanthomonas oryzae pv. oryzicola, suicide vector, knockout mutagenesis, hrp gene

ZOU Li-fang, LI Yu-rong , CHEN Gong-you. A Non-Marker Mutagenesis Strategy to Generate Poly-hrp Gene Mutants in the Rice Pathogen Xanthomonas oryzae pv. oryzicola[J]. Journal of Integrative Agriculture, 2011, 10(8): 1139-1150.

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A Non-Marker Mutagenesis Strategy to Generate Poly-hrp Gene Mutants in the Rice Pathogen Xanthomonas oryzae pv. oryzicola

A Non-Marker Mutagenesis Strategy to Generate Poly-hrp Gene Mutants in the Rice Pathogen Xanthomonas oryzae pv. oryzicola

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