Effects of light intensity on photosynthesis and photoprotective mechanisms in apple under progressive drought

doi:10.1016/S2095-3119(15)61148-0

摘要/Abstract

摘要： The effects of light intensity on photosynthesis and photoprotective mechanisms under progressive drought were studied on apple trees (Malus domestica Borkh.) Fuji. The potted trees were exposed to drought stress for 12 days and different light conditions (100, 60 and 25% sunlight). During the progressive drought, the relative water content (RWC) in leaf declined and was faster in full light than in 60 and 25% sunlight. However, the decrease in the net photosynthetic rate (Pn), stomatal conductance (Gs) and Rubisco activity were slower under 100% sunlight condition than other light conditions. After the 6 days of drought, the maximum PSII quantum yield (Fv/Fm), the capacity of electrons move beyond QA − (1–VJ) and electron move from intersystem to PSI acceptor side (1–VI)/(1–VJ) decreased, with greater decline extent in brighter light. While RWCs were >75%, the variations in different light intensities of Gs and Rubisco activity at identical RWC, suggested the direct effects of light. While the little difference in the state of photosynthetic electron transport chain among tested light intensities indicates the results of faster water loss rate of light. Our results also demonstrated that the enhancement the de-epoxidations of xanthophyll cycle, activities of ascorbate peroxidase (APX) and catalase (CAT) were directly regulated by light intensity. While the higher photorespiration rate (Pr) under stronger light condition was mainly caused by faster water loss rate of light.

关键词: apple , drought stress , photosynthesis , photoprotection

Abstract: The effects of light intensity on photosynthesis and photoprotective mechanisms under progressive drought were studied on apple trees (Malus domestica Borkh.) Fuji. The potted trees were exposed to drought stress for 12 days and different light conditions (100, 60 and 25% sunlight). During the progressive drought, the relative water content (RWC) in leaf declined and was faster in full light than in 60 and 25% sunlight. However, the decrease in the net photosynthetic rate (Pn), stomatal conductance (Gs) and Rubisco activity were slower under 100% sunlight condition than other light conditions. After the 6 days of drought, the maximum PSII quantum yield (Fv/Fm), the capacity of electrons move beyond QA − (1–VJ) and electron move from intersystem to PSI acceptor side (1–VI)/(1–VJ) decreased, with greater decline extent in brighter light. While RWCs were >75%, the variations in different light intensities of Gs and Rubisco activity at identical RWC, suggested the direct effects of light. While the little difference in the state of photosynthetic electron transport chain among tested light intensities indicates the results of faster water loss rate of light. Our results also demonstrated that the enhancement the de-epoxidations of xanthophyll cycle, activities of ascorbate peroxidase (APX) and catalase (CAT) were directly regulated by light intensity. While the higher photorespiration rate (Pr) under stronger light condition was mainly caused by faster water loss rate of light.

Key words: apple , drought stress , photosynthesis , photoprotection

MA Ping, BAI Tuan-hui, WANG Xiao-qian, MA Feng-wang. Effects of light intensity on photosynthesis and photoprotective mechanisms in apple under progressive drought[J]. Journal of Integrative Agriculture, 2015, 14(9): 1755-1766.

参考文献

Adams III W W, Muller O, Cohu C M, Demmig-Adams B. 2013.May photoinhibition be a consequence, rather than a cause,of limited plant productivity? Photosynthesis Research,117, 31-44

Aebi H. 1984. Catalase in vitro. Methods in Enzymology, 105,121-126

Arnon D L. 1949. Copper enzymes in isolated chloroplasts:Polyphenoloxidase in Betavulgaris L. Plant Physiology,24, 1-15

Asada K. 2006. Production and scavenging of reactive oxygenspecies in chloroplasts and their functions. Plant Physiology,141, 391-396

Bota J, Medrano H, Flexas J. 2004. Is photosynthesis limitedby decreased Rubisco activity and RuBP content underprogressive water stress? New Phytologist, 162, 671-681

Chen L S, Cheng L L. 2003. Carbon assimilation andcarbohydrate metabolism of ‘Concord’ grape (VitislabruscaL.) leaves in response to nitrogen supply. Journal of theAmerican Society for Horticultural Science, 128, 754-760

Cheng L L, Fuchigami L H. 2000. Rubisco activation statedecreases with increasing nitrogen content in apple leaves.Journal of Experimental Botany, 51, 1687-1694

Cornic G, Massacci A. 1994. Leaf photosynthesis underdrought stress. In: Baker N R, ed., Photosynthesis and theEnvironment. Kluwer Academic Publishers, Dordrecht, TheNetherlands. pp. 347-366

Corpas F J, Barroso J B, del Río L A. 2001. Peroxisomesas a source of reactive oxygen species and nitric oxidesignal molecules in plant cells. Trends in Plant Science,6, 145-150

Demmig B, Björkman O. 1987. Comparison of the effect ofexcessive light on chlorophyll fluorescence (77K) andphoton yield of O2 evolution in leaves of higher plants.Planta, 171, 171-184

Demmig-Adams B, Adams W. 1996. The role of xanthophyllcycle carotenoids in the protection of photosynthesis.Trends in Plant Science, 1, 21-26

Escalona J, Flexas J, Medrano H. 2000. Stomatal and nonstomatallimitations of photosynthesis under water stressin field-grown grapevines. Functional Plant Biology, 127,87-87

Farquhar G D, Sharkey T D. 1982. Stomatal conductance andphotosynthesis. Annual Review of Plant Physiology, 33,317-345

Flexas J, Medrano H. 2002. Drought inhibition of photosynthesisin C3 plants: Stomatal and non-stomatal limitationsrevisited. Annals of Botany, 89, 183-189

Giannopolitis C N, Ries S K. 1977. Superoxide dismutases. I.Occurrence in higher plants. Plant Physiology, 59, 309-314

Gilmore A M, Yamamoto H Y. 1991. Resolution of lutein andzeaxanthin using a non-endcapped, lightly carbon-loadedC18 high-performance liquid chromatographic column.Journal of Chromatography, 543, 137-145

Hager A. 1980. The reversible, light-induced conversions ofxanthophylls in the chloroplast. Pigments in Plants, 2,57-59

Kaiser W M. 1987. Effects of water deficit on photosyntheticcapacity. Physiologia Plantarum, 71, 142-149

Kramer D M, Johnson G, Kiirats O, Edwards G E. 2004. Newfluorescence parameters for the determination of QAredox state and excitation energy fluxes. PhotosynthesisResearch, 79, 209-218

Kruse O. 2001. Light-induced short-term adaptation mechanismsunder redox control in the PS II-LHCII supercomplex: LHC IIstate transitions and PS II repair cycle. Naturwissenschaften,88, 284-292

Li P M, Ma F W. 2012. Different effects of light irradiation onthe photosynthetic electron transport chain during appletree leaf dehydration. Plant Physiology and Biochemistry,55, 16-22

Lombardini L, Caspari H, Elfving D, Auvil T, McFerson J. 2002.Gas exchange and water relations in ‘FUJI’ apple treesgrown under deficit irrigation. Acta Horticulturae, 636,43-50

doi, 10.17660/ActaHortic.2004.636.4

Luo Y Y, Zhao X Y, Qu H, Zuo X A, Wang S K, Huang W D,Luo Y Q, Chen M. 2014. Photosynthetic performance andgrowth traits in Pennisetum centrasiaticum exposed todrought and rewatering under different soil nutrient regimes.Acta Physiologiae Plantarum, 36, 381-388

Ma P, Bai T H, Ma F W. 2015. Effects of progressive droughton photosynthesis and partitioning of absorbed light inapple trees. Journal of Integrative Agriculture, 14, 681-690

Marques da Silva J, Arrabac M C. 2004. Photosynthesis in thewater-stressed C4 grass Setaria sphacelata is mainly limitedby stomata with both rapidly and slowly imposed waterdeficits. Physiologia Plantarum, 121, 409-420

Maxwell K, Johnson G N. 2000. Chlorophyll fluorescencea practical guide. Journal of Experimental Botany, 51,659-668

Mullineaux C W, Emlyn-Jones D. 2005. State transitions:An example of acclimation to low-light stress. Journal ofExperimental Botany, 56, 389-393

Munné-Bosch S, Peñuelas J. 2004. Drought-induced oxidativestress in strawberry tree (Arbutus unedo L.) growingin Mediterranean field conditions. Plant Science, 166,1105-1110

Müller M, Hernández I, Alegre L, Munné-Bosch S. 2006.Enhanced α-tocopherol quinone levels and xanthophyllcycle de-epoxidation in rosemary plants exposed to waterdeficit during a Mediterranean winter. Journal of PlantPhysiology, 163, 601-606

Navari-Izzo F, Rascio N.1999. Plant response to water-deficitconditions. In: Pessarakli M, ed. Handbook of Plant andCrop Stress. Marcel Dekker Inc, New York. pp. 231-270

Parry M A J, Androlojc P J, Khan S, Lea P J, Keys A J. 2002.Rubisco activity: Effects of drought stress. Annals of Botany,89, 833-839

Peguero-Pina J J, Morales F, Flexas J, Gil-Pelegrín E, MoyaI. 2008. Photochemistry, remotely sensed physiologicalreflectance index and de-epoxidation state of thexanthophyll cycle in Quercus coccifera under intensedrought. Oecologia, 156, 1-11

Raki? T, Gaji? G, Lazarevi? M, Stevanovic B. 2015. Effects ofdifferent light intensities, CO2 concentrations, temperaturesand drought stress on photosynthetic activity in twopaleoendemic resurrection plant species Ramonda serbicaand R. nathaliae. Environmental and Experimental Botany,109, 63-72

Shimazaki K, Doi M, Assmann S M, Kinoshita T. 2007. Lightregulation of stomatal movement. Annual Review of PlantBiology, 58, 219-247

Siddique M, Hamid A, Islam M. 1999. Drought stress effectson photosynthetic rate and leaf gas exchange of wheat.Botanical Bulletin of Academia Sinica, 40, 141-145

Sircelj H, Tausz M, Grill D, Batic F. 2007. Detecting differentlevels of drought stress in apple trees (Malus domesticaBorkh.) with selected biochemical and physiologicalparameters. Scientia Horticulturae, 113, 362-369

Smirnoff. 1993. The role of active oxygen in the response ofplants to water de recit and desiccation. New Phytologist,125, 27-58

Strasser R J, Tsimilli-Michael M, Qiang S, Goltsev V. 2010.Simultaneous in vivo recording of prompt and delayedfluorescence and 820-nm reflection changes duringdrying and after rehydration of the resurrection plantHaberlearhodopensis. Biochimica et Biophysica Acta-Bioenergetics, 1797, 1313-1326

Wingler A, Quick W, Bungard R, Bailey K, Lea P, Leegood R.1999. The role of photorespiration during drought stress:An analysis utilizing barley mutants with reduced activitiesof photorespiratory enzymes. Plant Cell Environment, 22,361-373

Zhang N, Portis A R. 1999. Mechanism of light regulation ofRubisco: A specific role for the larger Rubisco activaseisoform involving reductive activation by thioredoxin-f.Proceedings of the National Academy of Sciences of theUnited States of America, 96, 9438-9443

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