Anatomical Study on the Multi-Ovule Development and Abortion of Hanfu Apple

doi:10.1016/S2095-3119(13)60409-8

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (4): 770-777.DOI: 10.1016/S2095-3119(13)60409-8

Anatomical Study on the Multi-Ovule Development and Abortion of Hanfu Apple

YANG Xu-yuan, MA Huai-yu, LIU Guo-cheng, LÜ De-guo, QIN Si-jun , DU Guo-dong

College of Horticulture, Shenyang Agricultural University/Key Laboratory for Northern Fruit Trees Cultivation and Physio-Ecology of Shenyang City, Shenyang 110866, P.R.China

收稿日期:2012-10-31 出版日期:2014-04-01 发布日期:2014-04-16
通讯作者: LIU Guo-cheng, Tel: +86-24-88487219; E-mail: liuguocheng2000@sina.com
作者简介:YANG Xu-yuan, E-mail: yangxuyuan320@sina.com; MA Huai-yu, Tel: +86-24-88487219, E-mail: shynydxgshzp@163.com
基金资助:
This study was supported by the Key Scientific and Technological Research Project of Shenyang City, China (1071154-3-00, F12-109-3-00) and the China Agriculture Research System (CARS-28).

YANG Xu-yuan, MA Huai-yu, LIU Guo-cheng, LÜ De-guo, QIN Si-jun , DU Guo-dong

College of Horticulture, Shenyang Agricultural University/Key Laboratory for Northern Fruit Trees Cultivation and Physio-Ecology of Shenyang City, Shenyang 110866, P.R.China

Received:2012-10-31 Online:2014-04-01 Published:2014-04-16
Contact: LIU Guo-cheng, Tel: +86-24-88487219; E-mail: liuguocheng2000@sina.com
About author:YANG Xu-yuan, E-mail: yangxuyuan320@sina.com; MA Huai-yu, Tel: +86-24-88487219, E-mail: shynydxgshzp@163.com
Supported by:
This study was supported by the Key Scientific and Technological Research Project of Shenyang City, China (1071154-3-00, F12-109-3-00) and the China Agriculture Research System (CARS-28).

摘要/Abstract

摘要： The terminal flower buds of 6-yr-old Hanfu apple were used to study the ovule development, ovular characteristics, cell death of abortive ovules, and dynamic change of starch grain quantity in the embryo sac with paraffin slices and terminal deoxynucleotidyl transferase-mediated fluorescein deoxyuridine triphosphate nick-end labeling (TUNEL) system. Four anatropous ovules in each ventricle could be observed before flowering. With the developing of floral organ, the bulk of normal ovules enlarged in each ventricle, the mature embryo sac differentiated into nucellus, and the egg cell developed into zygote by double fertilization. A large number of starch grains were observed during pollen tube growth and double fertilization, which guaranteed basic nutrient supply in the normal development of ovules. Moreover, abortion phenomenon of runtish ovules emerged at the stages of mature embryo sac, double fertilization and zygote development. The abortion characteristics included deformity of ovule development, degradation of nucellus tissue, separation between funiculus and ovule, abnormality of four-nucleate embryo sac, as well as development interruption of mature embryo sac. TUNEL analysis proved that ovule abortion was programmed cell death.

关键词: Hanfu apple , multi-ovule , abortion , programmed cell death , starch grain

Abstract: The terminal flower buds of 6-yr-old Hanfu apple were used to study the ovule development, ovular characteristics, cell death of abortive ovules, and dynamic change of starch grain quantity in the embryo sac with paraffin slices and terminal deoxynucleotidyl transferase-mediated fluorescein deoxyuridine triphosphate nick-end labeling (TUNEL) system. Four anatropous ovules in each ventricle could be observed before flowering. With the developing of floral organ, the bulk of normal ovules enlarged in each ventricle, the mature embryo sac differentiated into nucellus, and the egg cell developed into zygote by double fertilization. A large number of starch grains were observed during pollen tube growth and double fertilization, which guaranteed basic nutrient supply in the normal development of ovules. Moreover, abortion phenomenon of runtish ovules emerged at the stages of mature embryo sac, double fertilization and zygote development. The abortion characteristics included deformity of ovule development, degradation of nucellus tissue, separation between funiculus and ovule, abnormality of four-nucleate embryo sac, as well as development interruption of mature embryo sac. TUNEL analysis proved that ovule abortion was programmed cell death.

Key words: Hanfu apple , multi-ovule , abortion , programmed cell death , starch grain

YANG Xu-yuan, MA Huai-yu, LIU Guo-cheng, Lü De-guo, QIN Si-jun , DU Guo-dong. Anatomical Study on the Multi-Ovule Development and Abortion of Hanfu Apple[J]. Journal of Integrative Agriculture, 2014, 13(4): 770-777.

YANG Xu-yuan, MA Huai-yu, LIU Guo-cheng, Lü De-guo, QIN Si-jun , DU Guo-dong. YANG Xu-yuan, MA Huai-yu, LIU Guo-cheng, LÜ De-guo, QIN Si-jun , DU Guo-dong[J]. Journal of Integrative Agriculture, 2014, 13(4): 770-777.

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Anatomical Study on the Multi-Ovule Development and Abortion of Hanfu Apple

YANG Xu-yuan, MA Huai-yu, LIU Guo-cheng, LÜ De-guo, QIN Si-jun , DU Guo-dong

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