Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (5): 919-925.DOI: 10.1016/S2095-3119(13)60601-2

• 论文 •    下一篇

Proteomic Analysis of Wheat Seed in Response to Drought Stress

 ZHANG Yu-feng, HUANG Xiu-wen, WANG Li-li, WEI Liu, WU Zhi-hui, YOU Ming-shan , LI Bao-yun   

  1. Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis & Utilization, Ministry of Education/College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, P.R.China
  • 收稿日期:2013-03-07 出版日期:2014-05-01 发布日期:2014-05-06
  • 通讯作者: LI Bao-yun, Tel: +86-10-62731064, Fax: +86-10-62732568, E-mail: baoyunli@cau.edu.cn
  • 基金资助:

    This work was supported by the National Natural Science Foundation of China (31071412), the High-Tech R&D Program of China (2012AA101105) and the Key Basic Research Program of China (2009CB118300).

Proteomic Analysis of Wheat Seed in Response to Drought Stress

 ZHANG Yu-feng, HUANG Xiu-wen, WANG Li-li, WEI Liu, WU Zhi-hui, YOU Ming-shan , LI Bao-yun   

  1. Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis & Utilization, Ministry of Education/College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, P.R.China
  • Received:2013-03-07 Online:2014-05-01 Published:2014-05-06
  • Contact: LI Bao-yun, Tel: +86-10-62731064, Fax: +86-10-62732568, E-mail: baoyunli@cau.edu.cn
  • Supported by:

    This work was supported by the National Natural Science Foundation of China (31071412), the High-Tech R&D Program of China (2012AA101105) and the Key Basic Research Program of China (2009CB118300).

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摘要： Drought stress is one of the major factors affecting in wheat yield and grain quality. In order to investigate how drought stress might influence wheat quality during grain filling, three wheat cultivars Gaocheng 8901, Jagger and Nongda 3406 were subjected to drought stress during the grain filling stage. Neither globulin and glutenin, nor the relative percentage of amylose significantly changed following drought treatments, whereas albumin and gliadin concentrations did. The SDS-sedimentation, which has a strong linear correlation with wheat baking quality was markedly decreased following drought stress. These results indicated that drought had an adverse effect on wheat quality. In order to investigate the protein complexes in the wheat flour, the data from native PAGE and SDS-PAGE were combined and a total of 14 spots were successfully identified, and of these eight protein types were determined to be potential complex forming proteins.

关键词: wheat seed , drought stress , two-dimensional electrophoresis (2-DE)

Abstract: Drought stress is one of the major factors affecting in wheat yield and grain quality. In order to investigate how drought stress might influence wheat quality during grain filling, three wheat cultivars Gaocheng 8901, Jagger and Nongda 3406 were subjected to drought stress during the grain filling stage. Neither globulin and glutenin, nor the relative percentage of amylose significantly changed following drought treatments, whereas albumin and gliadin concentrations did. The SDS-sedimentation, which has a strong linear correlation with wheat baking quality was markedly decreased following drought stress. These results indicated that drought had an adverse effect on wheat quality. In order to investigate the protein complexes in the wheat flour, the data from native PAGE and SDS-PAGE were combined and a total of 14 spots were successfully identified, and of these eight protein types were determined to be potential complex forming proteins.

Key words: wheat seed , drought stress , two-dimensional electrophoresis (2-DE)