Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (12): 2584-2593.DOI: 10.1016/S2095-3119(14)60775-9

• 论文 • 上一篇    下一篇

GmPHR1, a Novel Homolog of the AtPHR1 Transcription Factor, Plays a Role in Plant Tolerance to Phosphate Starvation

 LI Xi-huan, WANG Yun-jie, WU Bing, KONG You-bin, LI Wen-long, CHANG Wen-suo , ZHANG Cai-ying   

  1. North China Key Laboratory for Germplasm Resources, Ministry of Education/Agricultural University of Hebei, Baoding 071001, P.R.China
  • 收稿日期:2013-10-13 出版日期:2014-12-01 发布日期:2014-12-10
  • 通讯作者: ZHANG Cai-ying,Tel: +86-312-7521558, Fax: +86-312-7528100, E-mail: cyzhang_60@126.com
  • 作者简介:LI Xi-huan, Mobile: 13930826741, Tel: +86-312-7528415, Fax: +86-312-7528100, E-mail: lixihuan@hebau.edu.cn
  • 基金资助:

    This work was supported by the National Natural Science Foundation of China (31071441, 31401405) and the National Transgenic Major Project of China (2014ZX0800404B).

GmPHR1, a Novel Homolog of the AtPHR1 Transcription Factor, Plays a Role in Plant Tolerance to Phosphate Starvation

 LI Xi-huan, WANG Yun-jie, WU Bing, KONG You-bin, LI Wen-long, CHANG Wen-suo , ZHANG Cai-ying   

  1. North China Key Laboratory for Germplasm Resources, Ministry of Education/Agricultural University of Hebei, Baoding 071001, P.R.China
  • Received:2013-10-13 Online:2014-12-01 Published:2014-12-10
  • Contact: ZHANG Cai-ying,Tel: +86-312-7521558, Fax: +86-312-7528100, E-mail: cyzhang_60@126.com
  • About author:LI Xi-huan, Mobile: 13930826741, Tel: +86-312-7528415, Fax: +86-312-7528100, E-mail: lixihuan@hebau.edu.cn
  • Supported by:

    This work was supported by the National Natural Science Foundation of China (31071441, 31401405) and the National Transgenic Major Project of China (2014ZX0800404B).

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摘要： GmPHR1 from soybean (Glycine max) was isolated and characterized. This novel homolog of the AtPHR1 transcription factor confers tolerance to inorganic phosphate (Pi)-starvation. The gene is 2751 bp long, with an 819-bp open reading frame and five introns. Analysis of transcription activity in yeast revealed that the full-length GmPHR1 and its C-terminal activate the reporter genes for His, Ade and Ura, suggesting that the C-terminal peptide functions as a transcriptional activator. Quantitative real-time PCR indicated that patterns of GmPHR1 expression differed. For example, under low-Pi stress, this gene was quickly induced in the tolerant JD11 after 0.5 h, with expression then decreasing slowly before peaking at 12-24 h. By contrast, induction in the sensitive Niumaohuang (NMH) was slow, peaking at 6 h before decreasing quickly at 9 h. GmPHR1 showed sub-cellular localization in the nuclei of onion epidermal cells and Arabidopsis roots. Growth parameters in wild-type (WT) Arabidopsis plants as well as in overexpression (OE) transgenic lines were examined. Under low-Pi conditions, values for shoot, root and whole-plant dry weights, root to shoot ratios, and lengths of primary roots were significantly greater in OE lines than in the WT. These data demonstrate that GmPHR1 has an important role in conferring tolerance to phosphate starvation.

关键词: GmPHR1 , transcription factor , AtPHR1 , phosphate starvation , low phosphate-stress tolerance , soybean

Abstract: GmPHR1 from soybean (Glycine max) was isolated and characterized. This novel homolog of the AtPHR1 transcription factor confers tolerance to inorganic phosphate (Pi)-starvation. The gene is 2751 bp long, with an 819-bp open reading frame and five introns. Analysis of transcription activity in yeast revealed that the full-length GmPHR1 and its C-terminal activate the reporter genes for His, Ade and Ura, suggesting that the C-terminal peptide functions as a transcriptional activator. Quantitative real-time PCR indicated that patterns of GmPHR1 expression differed. For example, under low-Pi stress, this gene was quickly induced in the tolerant JD11 after 0.5 h, with expression then decreasing slowly before peaking at 12-24 h. By contrast, induction in the sensitive Niumaohuang (NMH) was slow, peaking at 6 h before decreasing quickly at 9 h. GmPHR1 showed sub-cellular localization in the nuclei of onion epidermal cells and Arabidopsis roots. Growth parameters in wild-type (WT) Arabidopsis plants as well as in overexpression (OE) transgenic lines were examined. Under low-Pi conditions, values for shoot, root and whole-plant dry weights, root to shoot ratios, and lengths of primary roots were significantly greater in OE lines than in the WT. These data demonstrate that GmPHR1 has an important role in conferring tolerance to phosphate starvation.

Key words: GmPHR1 , transcription factor , AtPHR1 , phosphate starvation , low phosphate-stress tolerance , soybean