Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (2): 349-357.DOI: 10.1016/S2095-3119(13)60405-0

• 论文 • 上一篇    下一篇

Putrescine Plays a Positive Role in Salt-Tolerance Mechanisms by Reducing Oxidative Damage in Roots of Vegetable Soybean

 ZHANG Gu-wen, XU Sheng-chun, HU Qi-zan, MAO Wei-hua , GONG Ya-ming   

  1. 1.Institute of Vegetable, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, P.R.China
    2.Center of Analysis and Measurement, Zhejiang University, Hangzhou 310029, P.R.China
  • 收稿日期:2012-10-31 出版日期:2014-02-01 发布日期:2014-02-06
  • 通讯作者: GONG Ya-ming, Tel/Fax: +86-571-86404179,E-mail:gongym07@126.com
  • 作者简介:ZHANG Gu-wen, Tel: +86-571-86416055, Fax: +86-571-86404363, E-mail: zhangguwen@126.com
  • 基金资助:

    This work was supported by the National Natural Science Foundation of China (31101538, 31000942 and 31000676), the Grand Science and Technology Special Project of Zhejiang Province, China (2010C02006) and the Public Welfare Project of Zhejiang Province, China (2011R23A52D04).

Putrescine Plays a Positive Role in Salt-Tolerance Mechanisms by Reducing Oxidative Damage in Roots of Vegetable Soybean

 ZHANG Gu-wen, XU Sheng-chun, HU Qi-zan, MAO Wei-hua , GONG Ya-ming   

  1. 1.Institute of Vegetable, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, P.R.China
    2.Center of Analysis and Measurement, Zhejiang University, Hangzhou 310029, P.R.China
  • Received:2012-10-31 Online:2014-02-01 Published:2014-02-06
  • Contact: GONG Ya-ming, Tel/Fax: +86-571-86404179,E-mail:gongym07@126.com
  • About author:ZHANG Gu-wen, Tel: +86-571-86416055, Fax: +86-571-86404363, E-mail: zhangguwen@126.com
  • Supported by:

    This work was supported by the National Natural Science Foundation of China (31101538, 31000942 and 31000676), the Grand Science and Technology Special Project of Zhejiang Province, China (2010C02006) and the Public Welfare Project of Zhejiang Province, China (2011R23A52D04).

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摘要： Polyamines play important roles in plant tolerance to environmental stress. With the aim of investigating the possible involvement of putrescine (Put) in salt-tolerance mechanisms in vegetable soybean roots, exogenous Put (10 mmol L-1) and its biosynthetic inhibitor D-arginine (D-Arg) (0.5 mmol L-1) were added to nutrient solution when vegetable soybean (Glycine max L. cv. Huning 95-1) seedlings were exposed to 100 mmol L-1 sodium chloride (NaCl). The results showed that Put ameliorated but D-Arg aggravated the detrimental effects of NaCl on plant growth and biomass production. Under NaCl stress, levels of free, soluble conjugated and insoluble bound types of Put in roots of vegetable soybean were reduced, whereas those of free, soluble conjugated, and insoluble bound types of spermidine (Spd) and spermine (Spm) were increased. Exogenous Put eliminated the decrease in Put but promoted the increase of Spd and Spm. However, these changes could be reversed by D-Arg. Under NaCl stress, activities of arginine decarboxylase (ADC), S-adenosylmethionine decarboxylase (SAMDC), diamine oxidase (DAO), and polyamine oxidase (PAO) were induced, with exogenous Put promoting and D-Arg reversing these changes. Furthermore, NaCl stress decreased activities of antioxidant enzymes. Exogenous Put alleviated but D-Arg exaggerated these effects of NaCl stress, resulting in the same changes in membrane damage and reactive oxygen species (ROS) production. These results indicated that Put plays a positive role in vegetable soybean roots by activating antioxidant enzymes and thereby attenuating oxidative damage.

关键词: NaCl stress , oxidative damage , putrescine , salt-tolerance , vegetable soybean

Abstract: Polyamines play important roles in plant tolerance to environmental stress. With the aim of investigating the possible involvement of putrescine (Put) in salt-tolerance mechanisms in vegetable soybean roots, exogenous Put (10 mmol L-1) and its biosynthetic inhibitor D-arginine (D-Arg) (0.5 mmol L-1) were added to nutrient solution when vegetable soybean (Glycine max L. cv. Huning 95-1) seedlings were exposed to 100 mmol L-1 sodium chloride (NaCl). The results showed that Put ameliorated but D-Arg aggravated the detrimental effects of NaCl on plant growth and biomass production. Under NaCl stress, levels of free, soluble conjugated and insoluble bound types of Put in roots of vegetable soybean were reduced, whereas those of free, soluble conjugated, and insoluble bound types of spermidine (Spd) and spermine (Spm) were increased. Exogenous Put eliminated the decrease in Put but promoted the increase of Spd and Spm. However, these changes could be reversed by D-Arg. Under NaCl stress, activities of arginine decarboxylase (ADC), S-adenosylmethionine decarboxylase (SAMDC), diamine oxidase (DAO), and polyamine oxidase (PAO) were induced, with exogenous Put promoting and D-Arg reversing these changes. Furthermore, NaCl stress decreased activities of antioxidant enzymes. Exogenous Put alleviated but D-Arg exaggerated these effects of NaCl stress, resulting in the same changes in membrane damage and reactive oxygen species (ROS) production. These results indicated that Put plays a positive role in vegetable soybean roots by activating antioxidant enzymes and thereby attenuating oxidative damage.

Key words: NaCl stress , oxidative damage , putrescine , salt-tolerance , vegetable soybean