Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (2): 262-270.DOI: 10.1016/S2095-3119(15)61048-6

• 论文 • 上一篇    下一篇

The identification of presence/absence variants associated with the apparent differences of growth period structures between cultivated and wild soybeans

 LI Yan-fei, HONG Hui-long, LI Ying-hui, MA Yan-song, CHANG Ru-zhen, QIU Li-juan   

  1. 1、National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI)/Key Lab of Germplasm Utilization, Ministry of
    Agriculture/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
    2、College of Agriculture, Northeast Agricultural University, Harbin 150030, P.R.China
    3、Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, P.R.China
  • 收稿日期:2015-01-26 出版日期:2016-02-05 发布日期:2016-02-01
  • 通讯作者: LI Ying-hui, Tel/Fax: +86-10-82105843,E-mail: liyinghui@caas.cn; QIU Li-juan, Tel/Fax: +86-10-82105843,E-mail: qiulijuan@caas.cn
  • 作者简介:These authors contributed equally to this study.
  • 基金资助:

    This research was supported by the Agricultural Science and Technology Innovation Program (ASTIP) of Chinese Academy of Agricultural Sciences and the Platform of National Crop Germplasm Resources of China (nos. 2012-004 and 2013-004).

The identification of presence/absence variants associated with the apparent differences of growth period structures between cultivated and wild soybeans

 LI Yan-fei, HONG Hui-long, LI Ying-hui, MA Yan-song, CHANG Ru-zhen, QIU Li-juan   

  1. 1、National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI)/Key Lab of Germplasm Utilization, Ministry of
    Agriculture/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
    2、College of Agriculture, Northeast Agricultural University, Harbin 150030, P.R.China
    3、Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, P.R.China
  • Received:2015-01-26 Online:2016-02-05 Published:2016-02-01
  • Contact: LI Ying-hui, Tel/Fax: +86-10-82105843,E-mail: liyinghui@caas.cn; QIU Li-juan, Tel/Fax: +86-10-82105843,E-mail: qiulijuan@caas.cn
  • About author:These authors contributed equally to this study.
  • Supported by:

    This research was supported by the Agricultural Science and Technology Innovation Program (ASTIP) of Chinese Academy of Agricultural Sciences and the Platform of National Crop Germplasm Resources of China (nos. 2012-004 and 2013-004).

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摘要： The cultivated soybean (Glycine max (L.) Merr.) was distinguished from its wild progenitor Glycine soja Sieb. & Zucc. in growth period structure, by a shorter vegetative phase (V), a prolonged reproductive phase (R) and hence a larger R/V ratio. However, the genetic basis of the domestication of soybean from wild materials is unclear. Here, a panel of 123 cultivated and 97 wild accessions were genotyped using a set of 24 presence/absence variants (PAVs) while at the same time the materials were phenotyped with respect to flowering and maturity times at two trial sites located at very different latitudes. The major result of this study showed that variation at PAVs is informative for assessing patterns of genetic diversity in Glycine spp. The genotyping was largely consistent with the taxonomic status, although a few accessions were intermediate between the two major clades identified. Allelic diversity was much higher in the wild germplasm than in the cultivated materials. A significant domestication signal was detected at 11 of the PAVs at 0.01 level. In particular, this study has provided information for revealing the genetic basis of photoperiodism which was a prominent feature for the domestication of soybean. A significant marker-trait association with R/V ratio was detected at 14 of the PAVs, but stripping out population structure reduced this to three. These results will provide markers information for further finding of R/V related genes that can help to understand the domestication process and introgress novel genes in wild soybean to broaden the genetic base of modern soybean cultivars.

关键词: wild soybean , cultivated soybean , genomic structure variation , interspecific differentiation , photoperiod

Abstract: The cultivated soybean (Glycine max (L.) Merr.) was distinguished from its wild progenitor Glycine soja Sieb. & Zucc. in growth period structure, by a shorter vegetative phase (V), a prolonged reproductive phase (R) and hence a larger R/V ratio. However, the genetic basis of the domestication of soybean from wild materials is unclear. Here, a panel of 123 cultivated and 97 wild accessions were genotyped using a set of 24 presence/absence variants (PAVs) while at the same time the materials were phenotyped with respect to flowering and maturity times at two trial sites located at very different latitudes. The major result of this study showed that variation at PAVs is informative for assessing patterns of genetic diversity in Glycine spp. The genotyping was largely consistent with the taxonomic status, although a few accessions were intermediate between the two major clades identified. Allelic diversity was much higher in the wild germplasm than in the cultivated materials. A significant domestication signal was detected at 11 of the PAVs at 0.01 level. In particular, this study has provided information for revealing the genetic basis of photoperiodism which was a prominent feature for the domestication of soybean. A significant marker-trait association with R/V ratio was detected at 14 of the PAVs, but stripping out population structure reduced this to three. These results will provide markers information for further finding of R/V related genes that can help to understand the domestication process and introgress novel genes in wild soybean to broaden the genetic base of modern soybean cultivars.

Key words: wild soybean , cultivated soybean , genomic structure variation , interspecific differentiation , photoperiod