Sequencing and Phylogenetic Analysis of Potato virus Y Liaoning Isolate in China

doi:10.1016/S2095-3119(13)60442-6

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (7): 1195-1200.DOI: 10.1016/S2095-3119(13)60442-6

Sequencing and Phylogenetic Analysis of Potato virus Y Liaoning Isolate in China

WANG Fang, GAO Zheng-liang, AN Meng-nan, ZHOU Ben-guo , WU Yuan-hua

1.College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, P.R.China
2.Tobacco Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, P.R.China

收稿日期:2012-11-12 出版日期:2013-07-01 发布日期:2013-07-04
通讯作者: Correspondence WU Yuan-hua, Tel: +86-24-88487359, Fax: +86-24-88487359, E-mail: wuyh09@vip.sina.com
作者简介:WANG Fang, Tel: +86-551-65148963, Fax: +86-551-65148963, E-mail: wangfang1378@126.com;
基金资助:
This work was supported by the Programs for Science and Technology of State Tobacco Monopoly Administration, China ([2010]182) and the Science and Technology of Liaoning Province Tobacco Monopoly Administration, China ([2010]86) and the Key Programe of Anhui Province Tobacco Monopoly Administration, China (20100551005).

Sequencing and Phylogenetic Analysis of Potato virus Y Liaoning Isolate in China

WANG Fang, GAO Zheng-liang, AN Meng-nan, ZHOU Ben-guo , WU Yuan-hua

1.College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, P.R.China
2.Tobacco Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, P.R.China

Received:2012-11-12 Online:2013-07-01 Published:2013-07-04
Contact: Correspondence WU Yuan-hua, Tel: +86-24-88487359, Fax: +86-24-88487359, E-mail: wuyh09@vip.sina.com
About author:WANG Fang, Tel: +86-551-65148963, Fax: +86-551-65148963, E-mail: wangfang1378@126.com;
Supported by:
This work was supported by the Programs for Science and Technology of State Tobacco Monopoly Administration, China ([2010]182) and the Science and Technology of Liaoning Province Tobacco Monopoly Administration, China ([2010]86) and the Key Programe of Anhui Province Tobacco Monopoly Administration, China (20100551005).

摘要/Abstract

摘要： Complete genome sequence of Potato virus Y Liaoning isolate (PVY-LN) causing tobacco vein necrosis symptoms were isolated from Liaoning Province in China. Genome sequences of PVY-LN was 9 714 nucleotides in length, excluding the 3´- terminal poly (A) tail. PVY-LN encodes a single long open reading frame (ORF) of polyprotein that is predicted to be cleaved into ten mature proteins by three viral proteases. No recombination can be predicted in PVY-LN sequences compared with that of the other PVY strains using Recombination Detection Programe v. 4.16 (RDP4). Complete genome sequence comparison and phylogenetic analysis indicated that PVY-LN is closely related to PVY necrosis strain (PVYN).

关键词: PVY Liaoning isolate , cloning , genome sequencing , phylogeny

Abstract: Complete genome sequence of Potato virus Y Liaoning isolate (PVY-LN) causing tobacco vein necrosis symptoms were isolated from Liaoning Province in China. Genome sequences of PVY-LN was 9 714 nucleotides in length, excluding the 3´- terminal poly (A) tail. PVY-LN encodes a single long open reading frame (ORF) of polyprotein that is predicted to be cleaved into ten mature proteins by three viral proteases. No recombination can be predicted in PVY-LN sequences compared with that of the other PVY strains using Recombination Detection Programe v. 4.16 (RDP4). Complete genome sequence comparison and phylogenetic analysis indicated that PVY-LN is closely related to PVY necrosis strain (PVYN).

Key words: PVY Liaoning isolate , cloning , genome sequencing , phylogeny

WANG Fang, GAO Zheng-liang, AN Meng-nan, ZHOU Ben-guo , WU Yuan-hua. Sequencing and Phylogenetic Analysis of Potato virus Y Liaoning Isolate in China[J]. Journal of Integrative Agriculture, 2013, 12(7): 1195-1200.

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Sequencing and Phylogenetic Analysis of Potato virus Y Liaoning Isolate in China

Sequencing and Phylogenetic Analysis of Potato virus Y Liaoning Isolate in China

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