Cloning, Tissue Distribution, and Transmembrane Orientation of the Olfactory Co-Receptor Orco from Two Important Lepidopteran Rice Pests, the Leaffolder (Cnaphalocrocis medinalis) and the Striped Stem Borer (Chilo suppressalis)

doi:10.1016/S2095-3119(13)60501-8

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (10): 1816-1825.DOI: 10.1016/S2095-3119(13)60501-8

Cloning, Tissue Distribution, and Transmembrane Orientation of the Olfactory Co-Receptor Orco from Two Important Lepidopteran Rice Pests, the Leaffolder (Cnaphalocrocis medinalis) and the Striped Stem Borer (Chilo suppressalis)

LIU Su, HUANG Yuan-jie, QIAO Fei, ZHOU Wen-wu, GONG Zhong-jun, CHENG Jia-an , ZHU Zeng-rong

1 State Key Laboratory of Rice Biology/Key Laboratory of Agricultural Entomology, Ministry of Agriculture/Institute of Insect Sciences,
Zhejiang University, Hangzhou 310058, P.R.China
2 Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, P.R.China

收稿日期:2012-12-25 出版日期:2013-10-01 发布日期:2013-10-01
通讯作者: Correspondence ZHU Zeng-rong, Tel/Fax: +86-571-88982355, E-mail: zrzhu@zju.edu.cn
基金资助:
This work was funded by the Industry Project of Ministry of Agriculture of China (200903051) and the National Natural Science Foundation of China (30900149).

Cloning, Tissue Distribution, and Transmembrane Orientation of the Olfactory Co-Receptor Orco from Two Important Lepidopteran Rice Pests, the Leaffolder (Cnaphalocrocis medinalis) and the Striped Stem Borer (Chilo suppressalis)

LIU Su, HUANG Yuan-jie, QIAO Fei, ZHOU Wen-wu, GONG Zhong-jun, CHENG Jia-an , ZHU Zeng-rong

1 State Key Laboratory of Rice Biology/Key Laboratory of Agricultural Entomology, Ministry of Agriculture/Institute of Insect Sciences,
Zhejiang University, Hangzhou 310058, P.R.China
2 Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, P.R.China

Received:2012-12-25 Online:2013-10-01 Published:2013-10-01
Contact: Correspondence ZHU Zeng-rong, Tel/Fax: +86-571-88982355, E-mail: zrzhu@zju.edu.cn
Supported by:
This work was funded by the Industry Project of Ministry of Agriculture of China (200903051) and the National Natural Science Foundation of China (30900149).

摘要/Abstract

摘要： In insects, the sense of smell is mainly mediated by olfactory receptors (Ors). Olfactory co-receptor (Orco), which is coexpressed with the Ors in almost all olfactory receptor neurons (ORNs), is demonstrated to be an essential component in the insect olfactory system. It can be potential target for developing novel olfactory-disruption strategy to control insect pests. In this study, two full-length cDNA sequences encoding Orcos (CmedOrco and ChsupOrco) were cloned from two Lepidopteran rice pests, the rice leaffolder, Cnaphalocrocis medinalis and the rice striped stem borer, Chilo suppressalis. The amino acid sequences of CmedOrco and ChsupOrco showed high similarity to the previously identified Orcos from other insect species. Bioinformatic prediction and cellular immunofluorescence indicated that CmedOrco and ChsupOrco were both seventransmembrane proteins with intracellular N-termini and extracellular C-termini. mRNA expression levels of the two Orcos were much higher in male and female antennae than those in non-olfactory tissues, and the ChsupOrco transcripts reached a peak level in adults compared to other life stages. Our results provide a foundation from which it will be possible to elucidate the roles of Orco in moth olfaction and for the development of environment-friendly management strategies of these two rice insect pests.

关键词: Cnaphalocrocis medinalis , Chilo suppressalis , olfactory co-receptor , molecular cloning , relative expression , transmembrane orientation

Abstract: In insects, the sense of smell is mainly mediated by olfactory receptors (Ors). Olfactory co-receptor (Orco), which is coexpressed with the Ors in almost all olfactory receptor neurons (ORNs), is demonstrated to be an essential component in the insect olfactory system. It can be potential target for developing novel olfactory-disruption strategy to control insect pests. In this study, two full-length cDNA sequences encoding Orcos (CmedOrco and ChsupOrco) were cloned from two Lepidopteran rice pests, the rice leaffolder, Cnaphalocrocis medinalis and the rice striped stem borer, Chilo suppressalis. The amino acid sequences of CmedOrco and ChsupOrco showed high similarity to the previously identified Orcos from other insect species. Bioinformatic prediction and cellular immunofluorescence indicated that CmedOrco and ChsupOrco were both seventransmembrane proteins with intracellular N-termini and extracellular C-termini. mRNA expression levels of the two Orcos were much higher in male and female antennae than those in non-olfactory tissues, and the ChsupOrco transcripts reached a peak level in adults compared to other life stages. Our results provide a foundation from which it will be possible to elucidate the roles of Orco in moth olfaction and for the development of environment-friendly management strategies of these two rice insect pests.

Key words: Cnaphalocrocis medinalis , Chilo suppressalis , olfactory co-receptor , molecular cloning , relative expression , transmembrane orientation

LIU Su, HUANG Yuan-jie, QIAO Fei, ZHOU Wen-wu, GONG Zhong-jun, CHENG Jia-an , ZHU Zeng-rong . Cloning, Tissue Distribution, and Transmembrane Orientation of the Olfactory Co-Receptor Orco from Two Important Lepidopteran Rice Pests, the Leaffolder (Cnaphalocrocis medinalis) and the Striped Stem Borer (Chilo suppressalis)[J]. Journal of Integrative Agriculture, 2013, 12(10): 1816-1825.

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Cloning, Tissue Distribution, and Transmembrane Orientation of the Olfactory Co-Receptor Orco from Two Important Lepidopteran Rice Pests, the Leaffolder (Cnaphalocrocis medinalis) and the Striped Stem Borer (Chilo suppressalis)

Cloning, Tissue Distribution, and Transmembrane Orientation of the Olfactory Co-Receptor Orco from Two Important Lepidopteran Rice Pests, the Leaffolder (Cnaphalocrocis medinalis) and the Striped Stem Borer (Chilo suppressalis)

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