Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (1): 103-109.DOI: 10.1016/S2095-3119(13)60210-5

• 论文 • 上一篇    下一篇

Characterization of Porcine Matrix Metalloproteinase 23 (pMMP-23) Gene and Its Association with Litter Size Traits

 NIU Bu-yue, LAN Xiao-ming, YAN Xiao-hong, DI Sheng-wei, WANG Yang, LI Hai-tao, XIONG Yuanzhu, WANG Xi-biao   

  1. 1.College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, P.R.China
    2.Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture/Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education/Huazhong Agricultural University, Wuhan 430070, P.R.China
  • 收稿日期:2011-11-29 出版日期:2013-01-01 发布日期:2013-01-24
  • 通讯作者: Correspondence WANG Xi-biao, Tel: +86-451-55191109, E-mail: wxibiao1967@yahoo.com.cn
  • 作者简介:NIU Bu-yue, E-mail: niubuyue@163.com
  • 基金资助:

    This study was supported by the Educational Commission of Heilongjiang Province, China (11541031).

Characterization of Porcine Matrix Metalloproteinase 23 (pMMP-23) Gene and Its Association with Litter Size Traits

 NIU Bu-yue, LAN Xiao-ming, YAN Xiao-hong, DI Sheng-wei, WANG Yang, LI Hai-tao, XIONG Yuanzhu, WANG Xi-biao   

  1. 1.College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, P.R.China
    2.Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture/Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education/Huazhong Agricultural University, Wuhan 430070, P.R.China
  • Received:2011-11-29 Online:2013-01-01 Published:2013-01-24
  • Contact: Correspondence WANG Xi-biao, Tel: +86-451-55191109, E-mail: wxibiao1967@yahoo.com.cn
  • About author:NIU Bu-yue, E-mail: niubuyue@163.com
  • Supported by:

    This study was supported by the Educational Commission of Heilongjiang Province, China (11541031).

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摘要： The matrix metalloproteinase 23 (MMP-23), which might play a role in ovulation in mammals, was one of the promising candidate genes for litter size traits in pigs. In the present research, partial sequence of porcine MMP-23 (pMMP-23) gene, including exons 2-8 (GenBank: EU360790), was obtained. Real-time PCR analysis revealed that pMMP-23 gene was highly expressed in ovary. PCR-Sau3A I-RFLP and PCR-Acc II-RFLP assay were established to detect a C/T mutation in exon 3 (EU360790: g. 269C>T) and an A/G mutation in exon 4 (EU360790: g. 505A>G), respectively. Association study for these two SNPs with litter size was assessed in three independent populations (Minzhu, Landrace I and Landrace II). Statistical analysis demonstrated that for second and subsequent litters, TT sows produced more TNB than CC pigs in Landrace breed (P<0.05) at g. 269C>T locus, and the additive effect was significant (P<0.05); GG sows produced more TNB and NBA than AA pigs in Minzhu (P<0.01) and Landrace breeds (P<0.05) at g. 505A>G locus, and the additive effect was significant (P<0.01 or P<0.05). Our study suggested that the pMMP-23 gene might be a novel candidate gene for litter size traits, and g. 505A>G locus might be a useful molecular marker for marker assisted selection (MAS).

关键词: pig , MMP-23 , molecular cloning , expression , litter size

Abstract: The matrix metalloproteinase 23 (MMP-23), which might play a role in ovulation in mammals, was one of the promising candidate genes for litter size traits in pigs. In the present research, partial sequence of porcine MMP-23 (pMMP-23) gene, including exons 2-8 (GenBank: EU360790), was obtained. Real-time PCR analysis revealed that pMMP-23 gene was highly expressed in ovary. PCR-Sau3A I-RFLP and PCR-Acc II-RFLP assay were established to detect a C/T mutation in exon 3 (EU360790: g. 269C>T) and an A/G mutation in exon 4 (EU360790: g. 505A>G), respectively. Association study for these two SNPs with litter size was assessed in three independent populations (Minzhu, Landrace I and Landrace II). Statistical analysis demonstrated that for second and subsequent litters, TT sows produced more TNB than CC pigs in Landrace breed (P<0.05) at g. 269C>T locus, and the additive effect was significant (P<0.05); GG sows produced more TNB and NBA than AA pigs in Minzhu (P<0.01) and Landrace breeds (P<0.05) at g. 505A>G locus, and the additive effect was significant (P<0.01 or P<0.05). Our study suggested that the pMMP-23 gene might be a novel candidate gene for litter size traits, and g. 505A>G locus might be a useful molecular marker for marker assisted selection (MAS).

Key words: pig , MMP-23 , molecular cloning , expression , litter size