Identification of SSR Marker Linked to a Major Dwarfing Gene in Common Wheat

doi:10.1016/S2095-3119(13)60266-X

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (5): 749-755.DOI: 10.1016/S2095-3119(13)60266-X

Identification of SSR Marker Linked to a Major Dwarfing Gene in Common Wheat

MENG Ya-ning, KANG Su-hua, LAN Su-que, LI Xing-pu, ZHANG Ye-lun , BAI Feng

1.Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences/Hebei Research Station of Crop Gene Resource &Germplasm Enhancement, Ministry of Agriculture/Key Laboratory of Genetics and Breeding of Hebei Province, Shijiazhuang 050031,P.R.China
2.College of Life Science, Hebei Normal University, Shijiazhuang 050016, P.R.China

收稿日期:2012-09-17 出版日期:2013-05-01 发布日期:2013-05-01
通讯作者: Correspondence LI Xing-pu, Tel: +86-311-87670622, Fax: +86-311-87670663, E-mail: lixingpu@126.com
基金资助:
This work was supported by the Natural Science Foundation of Hebei Province, China (C200500637), the Key Technologies R&D Program of China during the 11th Five-Year Plan period (2006BAD13B02-08), and the Hebei Provincial Science and Technology Underpinning Project, China (06820119D).

Identification of SSR Marker Linked to a Major Dwarfing Gene in Common Wheat

MENG Ya-ning, KANG Su-hua, LAN Su-que, LI Xing-pu, ZHANG Ye-lun , BAI Feng

1.Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences/Hebei Research Station of Crop Gene Resource &Germplasm Enhancement, Ministry of Agriculture/Key Laboratory of Genetics and Breeding of Hebei Province, Shijiazhuang 050031,P.R.China
2.College of Life Science, Hebei Normal University, Shijiazhuang 050016, P.R.China

Received:2012-09-17 Online:2013-05-01 Published:2013-05-01
Contact: Correspondence LI Xing-pu, Tel: +86-311-87670622, Fax: +86-311-87670663, E-mail: lixingpu@126.com
Supported by:
This work was supported by the Natural Science Foundation of Hebei Province, China (C200500637), the Key Technologies R&D Program of China during the 11th Five-Year Plan period (2006BAD13B02-08), and the Hebei Provincial Science and Technology Underpinning Project, China (06820119D).

摘要/Abstract

摘要： A segregating population with 410 F2 individuals from the cross MERCIA (Rht-B1a) × Dwarf 123 was made to identify a new major dwarfing gene carrying by novel wheat germplasm Dwarf 123. Combination of bulk segerant analysis method was used. A total of 145 SSR markers were tested for polymorphisms among parental lines and DNA bulks of F2 population. Out of 145 primer pairs only three markers revealed corresponding polymorphism among parental lines and F2 DNA bulks. The marker Barc20 was close to the dwarfing gene with a genetic distance of 1.8 cM, and markers Gwm513 and Gwm495 were linked to the gene with genetic distance of 6.7 and 13 cM, respectively. Linkage analysis mapped the dwarfing gene to the long arm of chromosome 4B with the order of Barc20-dwarfing gene-Gwm513-Gwm495. The Comparision between the new gene and the known Rht-B1 alleles showed that dwarfing gene Rht-Ai123 was different from the others. The identification of the new dwarfing gene and its linked markers will greatly facilitate its utilization in wheat high yield breeding for reducing plant height.

关键词: chromosome , dwarfing gene , genetic distance , linkage analysis , molecular markers , polymorphism , Triticum aestivum L.

Abstract: A segregating population with 410 F2 individuals from the cross MERCIA (Rht-B1a) × Dwarf 123 was made to identify a new major dwarfing gene carrying by novel wheat germplasm Dwarf 123. Combination of bulk segerant analysis method was used. A total of 145 SSR markers were tested for polymorphisms among parental lines and DNA bulks of F2 population. Out of 145 primer pairs only three markers revealed corresponding polymorphism among parental lines and F2 DNA bulks. The marker Barc20 was close to the dwarfing gene with a genetic distance of 1.8 cM, and markers Gwm513 and Gwm495 were linked to the gene with genetic distance of 6.7 and 13 cM, respectively. Linkage analysis mapped the dwarfing gene to the long arm of chromosome 4B with the order of Barc20-dwarfing gene-Gwm513-Gwm495. The Comparision between the new gene and the known Rht-B1 alleles showed that dwarfing gene Rht-Ai123 was different from the others. The identification of the new dwarfing gene and its linked markers will greatly facilitate its utilization in wheat high yield breeding for reducing plant height.

Key words: chromosome , dwarfing gene , genetic distance , linkage analysis , molecular markers , polymorphism , Triticum aestivum L.

MENG Ya-ning, KANG Su-hua, LAN Su-que, LI Xing-pu, ZHANG Ye-lun , BAI Feng. Identification of SSR Marker Linked to a Major Dwarfing Gene in Common Wheat[J]. Journal of Integrative Agriculture, 2013, 12(5): 749-755.

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Identification of SSR Marker Linked to a Major Dwarfing Gene in Common Wheat

Identification of SSR Marker Linked to a Major Dwarfing Gene in Common Wheat

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