Alterations of Alternative Splicing Patterns of Ser/Arg-Rich (SR) Genes in Response to Hormones and Stresses Treatments in Different Ecotypes of Rice (Oryza sativa)

doi:10.1016/S2095-3119(13)60260-9

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (5): 737-748.DOI: 10.1016/S2095-3119(13)60260-9

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Alterations of Alternative Splicing Patterns of Ser/Arg-Rich (SR) Genes in Response to Hormones and Stresses Treatments in Different Ecotypes of Rice (Oryza sativa)

ZHANG Peng, DENG Heng, XIAO Fang-ming , LIU Yong-sheng

1.Key Laboratory for Bio-Resource and Eco-Environment, Ministry of Education/State Key Laboratory of Hydraulics and Mountain River
Engineering/College of Life Science, Sichuan University, Chengdu 610064, P.R.China
2.Neijiang Hybrid Rice Research and Development Center, Neijiang 641000, P.R.China
3.Department of Plant, Soil and Entomological Sciences, University of Idaho, Moscow 83844, USA
4.School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, P.R.China

收稿日期:2012-08-29 出版日期:2013-05-01 发布日期:2013-05-01
通讯作者: Correspondence LIU Yong-sheng, E-mail: liuyongsheng1122@yahoo.com.cn
作者简介:ZHANG Peng, E-mail: njzhp825@sohu.com
基金资助:
This work was supported by the National Basic Research Program of China (2011CB100401), the National Science Fund of China for Distinguished Young Scientists (30825030), the National Natural Science Foundation of China (30970260, 30770466 and 30971752), and the Key Project from Chongqing Local Government, China (2010AA1019).

Alterations of Alternative Splicing Patterns of Ser/Arg-Rich (SR) Genes in Response to Hormones and Stresses Treatments in Different Ecotypes of Rice (Oryza sativa)

ZHANG Peng, DENG Heng, XIAO Fang-ming , LIU Yong-sheng

1.Key Laboratory for Bio-Resource and Eco-Environment, Ministry of Education/State Key Laboratory of Hydraulics and Mountain River
Engineering/College of Life Science, Sichuan University, Chengdu 610064, P.R.China
2.Neijiang Hybrid Rice Research and Development Center, Neijiang 641000, P.R.China
3.Department of Plant, Soil and Entomological Sciences, University of Idaho, Moscow 83844, USA
4.School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, P.R.China

Received:2012-08-29 Online:2013-05-01 Published:2013-05-01
Contact: Correspondence LIU Yong-sheng, E-mail: liuyongsheng1122@yahoo.com.cn
About author:ZHANG Peng, E-mail: njzhp825@sohu.com
Supported by:
This work was supported by the National Basic Research Program of China (2011CB100401), the National Science Fund of China for Distinguished Young Scientists (30825030), the National Natural Science Foundation of China (30970260, 30770466 and 30971752), and the Key Project from Chongqing Local Government, China (2010AA1019).

摘要/Abstract

摘要： Ser/Arg-rich (SR) genes encode proteins that play pivotal roles in both constitutive and alternative splicing of pre-mRNA. However, not much effort has been made to investigate the alternative splicing of their own pre-mRNA. In this study, we conducted comprehensive analyses of pre-mRNA splicing for 22 SR genes in three rice (Oryza sativa L.) ecotypes indica, japonica and javanica. Using different ecotypes we characterized the variations in expression and splicing patterns of rice SR genes in different tissues and at different developmental stages. In addition, we compared the divergence in expression and splicing patterns of SR genes from seedlings of different rice ecotypes in response to hormones application and environmental stresses. Our results revealed the complexity of alternative splicing of SR genes in rice. The splicing varies in different tissues, in different ecotypes, in response to stresses and hormones. Thus, our study suggested that SR genes were subjected to sophisticated alternative splicing although their encoding proteins were involved in the splicing process.

关键词: SR protein , alternative splicing , stress , rice

Abstract: Ser/Arg-rich (SR) genes encode proteins that play pivotal roles in both constitutive and alternative splicing of pre-mRNA. However, not much effort has been made to investigate the alternative splicing of their own pre-mRNA. In this study, we conducted comprehensive analyses of pre-mRNA splicing for 22 SR genes in three rice (Oryza sativa L.) ecotypes indica, japonica and javanica. Using different ecotypes we characterized the variations in expression and splicing patterns of rice SR genes in different tissues and at different developmental stages. In addition, we compared the divergence in expression and splicing patterns of SR genes from seedlings of different rice ecotypes in response to hormones application and environmental stresses. Our results revealed the complexity of alternative splicing of SR genes in rice. The splicing varies in different tissues, in different ecotypes, in response to stresses and hormones. Thus, our study suggested that SR genes were subjected to sophisticated alternative splicing although their encoding proteins were involved in the splicing process.

Key words: SR protein , alternative splicing , stress , rice

ZHANG Peng, DENG Heng, XIAO Fang-ming , LIU Yong-sheng. Alterations of Alternative Splicing Patterns of Ser/Arg-Rich (SR) Genes in Response to Hormones and Stresses Treatments in Different Ecotypes of Rice (Oryza sativa)[J]. Journal of Integrative Agriculture, 2013, 12(5): 737-748.

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Alterations of Alternative Splicing Patterns of Ser/Arg-Rich (SR) Genes in Response to Hormones and Stresses Treatments in Different Ecotypes of Rice (Oryza sativa)

Alterations of Alternative Splicing Patterns of Ser/Arg-Rich (SR) Genes in Response to Hormones and Stresses Treatments in Different Ecotypes of Rice (Oryza sativa)

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