QTL Mapping for Adult Plant Resistance to Powdery Mildew in Italian Wheat cv. Strampelli

doi:10.1016/S2095-3119(13)60297-X

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (5): 756-764.DOI: 10.1016/S2095-3119(13)60297-X

QTL Mapping for Adult Plant Resistance to Powdery Mildew in Italian Wheat cv. Strampelli

Asad Muhammad Azeem, BAI Bin, LAN Cai-xia, YAN Jun, XIA Xian-chun, ZHANG Yong , HE Zhong-hu

1.National Wheat Improvement Center/National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences,Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, P.R.China
2.Cotton Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Anyang 455000, P.R.China
3.International Maize and Wheat Improvement Center (CIMMYT) China Office, Beijing 100081, P.R.China

收稿日期:2012-07-19 出版日期:2013-05-01 发布日期:2013-05-01
通讯作者: Correspondence HE Zhong-hu, Tel: +86-10-82108547, E-mail: zhhecaas@163.com
作者简介:Asad Muhammad Azeem, E-mail: azeem_nia@yahoo.com;
基金资助:
This study was supported by the National Basic Research Program of China (2013CB127700), the International Collaboration Project, Ministry of Agriculture, China (2011-G3), the National Natural Science Foundation of China (31261140370), and the China Agriculture Research System (CARS-3-1-3).

QTL Mapping for Adult Plant Resistance to Powdery Mildew in Italian Wheat cv. Strampelli

Asad Muhammad Azeem, BAI Bin, LAN Cai-xia, YAN Jun, XIA Xian-chun, ZHANG Yong , HE Zhong-hu

1.National Wheat Improvement Center/National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences,Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, P.R.China
2.Cotton Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Anyang 455000, P.R.China
3.International Maize and Wheat Improvement Center (CIMMYT) China Office, Beijing 100081, P.R.China

Received:2012-07-19 Online:2013-05-01 Published:2013-05-01
Contact: Correspondence HE Zhong-hu, Tel: +86-10-82108547, E-mail: zhhecaas@163.com
About author:Asad Muhammad Azeem, E-mail: azeem_nia@yahoo.com;
Supported by:
This study was supported by the National Basic Research Program of China (2013CB127700), the International Collaboration Project, Ministry of Agriculture, China (2011-G3), the National Natural Science Foundation of China (31261140370), and the China Agriculture Research System (CARS-3-1-3).

摘要/Abstract

摘要： The Italian wheat cv. Strampelli displays high resistance to powdery mildew caused by Blumeria graminis f. sp. tritici. The objective of this study was to map quantitative trait loci (QTLs) for resistance to powdery mildew in a population of 249 F2:3 lines from Strampelli/Huixianhong. Adult plant powdery mildew tests were conducted over 2 yr in Beijing and 1 yr in Anyang and simple sequence repeat (SSR) markers were used for genotyping. QTLs Qpm.caas-3BS, Qpm.caas-5BL.1, and Qpm.caas-7DS were consistent across environments whereas, Qpm.caas-2BS.1 found in two environments, explained 0.4-1.6, 5.5-6.9, 27.1-34.5, and 1.0-3.5% of the phenotypic variation respectively. Qpm.caas-7DS corresponded to the genomic location of Pm38/Lr34/Yr18. Qpm.caas-4BL was identified in Anyang 2010 and Beijing 2011, accounting for 1.9-3.5% of phenotypic variation. Qpm.caas-2BS.1 and Qpm.caas-5BL.1 contributed by Strampelli and Qpm.caas-3BS by Huixianhong, seem to be new QTL for powdery mildew resistance. Qpm.caas-4BL, Qpm.caas-5BL.3, and Qpm.caas-7DS contributed by Strampelli appeared to be in the same genomic regions as those mapped previously for stripe rust resistance in the same population, indicating that these loci conferred resistance to both stripe rust and powdery mildew. Strampelli could be a valuable genetic resource for improving durable resistance to both powdery mildew and stripe rust in wheat.

关键词: QTL analysis , SSR markers , Blumeria graminis , durable resistance , Triticum aestivum L.

Abstract: The Italian wheat cv. Strampelli displays high resistance to powdery mildew caused by Blumeria graminis f. sp. tritici. The objective of this study was to map quantitative trait loci (QTLs) for resistance to powdery mildew in a population of 249 F2:3 lines from Strampelli/Huixianhong. Adult plant powdery mildew tests were conducted over 2 yr in Beijing and 1 yr in Anyang and simple sequence repeat (SSR) markers were used for genotyping. QTLs Qpm.caas-3BS, Qpm.caas-5BL.1, and Qpm.caas-7DS were consistent across environments whereas, Qpm.caas-2BS.1 found in two environments, explained 0.4-1.6, 5.5-6.9, 27.1-34.5, and 1.0-3.5% of the phenotypic variation respectively. Qpm.caas-7DS corresponded to the genomic location of Pm38/Lr34/Yr18. Qpm.caas-4BL was identified in Anyang 2010 and Beijing 2011, accounting for 1.9-3.5% of phenotypic variation. Qpm.caas-2BS.1 and Qpm.caas-5BL.1 contributed by Strampelli and Qpm.caas-3BS by Huixianhong, seem to be new QTL for powdery mildew resistance. Qpm.caas-4BL, Qpm.caas-5BL.3, and Qpm.caas-7DS contributed by Strampelli appeared to be in the same genomic regions as those mapped previously for stripe rust resistance in the same population, indicating that these loci conferred resistance to both stripe rust and powdery mildew. Strampelli could be a valuable genetic resource for improving durable resistance to both powdery mildew and stripe rust in wheat.

Key words: QTL analysis , SSR markers , Blumeria graminis , durable resistance , Triticum aestivum L.

Asad Muhammad Azeem, BAI Bin, LAN Cai-xia, YAN Jun, XIA Xian-chun, ZHANG Yong , HE Zhong-hu. QTL Mapping for Adult Plant Resistance to Powdery Mildew in Italian Wheat cv. Strampelli[J]. Journal of Integrative Agriculture, 2013, 12(5): 756-764.

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QTL Mapping for Adult Plant Resistance to Powdery Mildew in Italian Wheat cv. Strampelli

QTL Mapping for Adult Plant Resistance to Powdery Mildew in Italian Wheat cv. Strampelli

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