Effects of Elevated Ozone Concentration on Starch and Starch Synthesis Enzymes of Yangmai 16 Under Fully Open-Air Field Conditions

doi:10.1016/S2095-3119(13)60345-7

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (12): 2157-2163.DOI: 10.1016/S2095-3119(13)60345-7

Effects of Elevated Ozone Concentration on Starch and Starch Synthesis Enzymes of Yangmai 16 Under Fully Open-Air Field Conditions

ZHANG Ru-biao, HU Hai-juan, ZHAO Zheng, YANG Dan-dan, ZHU Xin-kai, GUO Wen-shan

1 Key Lab of Crop Genetics and Physiology, Jiangsu Province/Wheat Research Institute, Yangzhou University, Yangzhou 225009, P.R.China
2 State Key Laboratory of Soil and Sustainable Agriculture, Ministry of Science and Technology/Institute of Soil Sciences, Chinese Academy of Sciences, Nanjing 210008, P.R.China
3 Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan

收稿日期:2012-10-29 出版日期:2013-12-01 发布日期:2013-12-17
通讯作者: ZHU Xin-kai, Tel/Fax: +86-514-87979300, E-mail: xkzhu@yzu.edu.cn; GUO Wen-shan, Tel: +86-514-87979339, E-mail: guows@yzu.edu.cn
基金资助:
This work was supported by the Innovation Program of Chinese Academy of Sciences (KZCX2-EW-414), the International S&T Cooperation Program of China (2009DFA31110), the Qing Lan Project Knowledge, Priority Academic Program Development of Jiangsu Higher Education Institutions, China and the Global Environment Research Fund, Ministry of the Environment, Japan (C- 062). We are grateful to Prof. Liu Gang, Chinese Academy of Sciences, for his technical support in the free-air ozone release system.

Effects of Elevated Ozone Concentration on Starch and Starch Synthesis Enzymes of Yangmai 16 Under Fully Open-Air Field Conditions

ZHANG Ru-biao, HU Hai-juan, ZHAO Zheng, YANG Dan-dan, ZHU Xin-kai, GUO Wen-shan

1 Key Lab of Crop Genetics and Physiology, Jiangsu Province/Wheat Research Institute, Yangzhou University, Yangzhou 225009, P.R.China
2 State Key Laboratory of Soil and Sustainable Agriculture, Ministry of Science and Technology/Institute of Soil Sciences, Chinese Academy of Sciences, Nanjing 210008, P.R.China
3 Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan

Received:2012-10-29 Online:2013-12-01 Published:2013-12-17
Contact: ZHU Xin-kai, Tel/Fax: +86-514-87979300, E-mail: xkzhu@yzu.edu.cn; GUO Wen-shan, Tel: +86-514-87979339, E-mail: guows@yzu.edu.cn
Supported by:
This work was supported by the Innovation Program of Chinese Academy of Sciences (KZCX2-EW-414), the International S&T Cooperation Program of China (2009DFA31110), the Qing Lan Project Knowledge, Priority Academic Program Development of Jiangsu Higher Education Institutions, China and the Global Environment Research Fund, Ministry of the Environment, Japan (C- 062). We are grateful to Prof. Liu Gang, Chinese Academy of Sciences, for his technical support in the free-air ozone release system.

摘要/Abstract

摘要： O3 is not only greenhouse gas but also a primary gaseous contaminant in the atmosphere. It has long-lasting effects on crop growth, yield and quality, and brings a series of ecological and environmental problems. A free-air controlled enrichment (FACE) system was applied to study the effect of elevated ozone concentration on activities of key enzymes of starch synthesis of Yangmai 16 in 2009-2010. The main-plot treatment had two levels of O3: ambient level (A-O3) and 50% higher than ambient level (E-O3). The main results were that accumulation rate of amylose, amylopectin and starch were represented in a single peak curve, and their content and accumulation amount rose gradually. The O3 elevation decreased the accumulation rate of amylose, amylopectin and starch amylase, reduced the accumulation amount of amylopectin and starch, and decreased the content of amylopectin and starch, but increased the content of amylose. With the increase of O3 concentration, the enzyme activity of grain granule-bound starch synthase (GBSS), soluble starch synthase (SSS) and starch branching enzyme (SBE) decreased after anthesis. The activities of GBSS and SSS had highly significant correlations with amylose, amylopectin and starch accumulation rate, and the activity of SBE had significant correlations with these items. So the O3 elevation decreased the activity of key enzymes of starch synthesis, which led to the variation of starch synthesis.

关键词: O3 , FACE , wheat , starch , activity of enzymes

Abstract: O3 is not only greenhouse gas but also a primary gaseous contaminant in the atmosphere. It has long-lasting effects on crop growth, yield and quality, and brings a series of ecological and environmental problems. A free-air controlled enrichment (FACE) system was applied to study the effect of elevated ozone concentration on activities of key enzymes of starch synthesis of Yangmai 16 in 2009-2010. The main-plot treatment had two levels of O3: ambient level (A-O3) and 50% higher than ambient level (E-O3). The main results were that accumulation rate of amylose, amylopectin and starch were represented in a single peak curve, and their content and accumulation amount rose gradually. The O3 elevation decreased the accumulation rate of amylose, amylopectin and starch amylase, reduced the accumulation amount of amylopectin and starch, and decreased the content of amylopectin and starch, but increased the content of amylose. With the increase of O3 concentration, the enzyme activity of grain granule-bound starch synthase (GBSS), soluble starch synthase (SSS) and starch branching enzyme (SBE) decreased after anthesis. The activities of GBSS and SSS had highly significant correlations with amylose, amylopectin and starch accumulation rate, and the activity of SBE had significant correlations with these items. So the O3 elevation decreased the activity of key enzymes of starch synthesis, which led to the variation of starch synthesis. Key words:

Key words: O3 , FACE , wheat , starch , activity of enzymes

ZHANG Ru-biao, HU Hai-juan, ZHAO Zheng, YANG Dan-dan, ZHU Xin-kai, GUO Wen-shan. Effects of Elevated Ozone Concentration on Starch and Starch Synthesis Enzymes of Yangmai 16 Under Fully Open-Air Field Conditions[J]. Journal of Integrative Agriculture, 2013, 12(12): 2157-2163.

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Effects of Elevated Ozone Concentration on Starch and Starch Synthesis Enzymes of Yangmai 16 Under Fully Open-Air Field Conditions

Effects of Elevated Ozone Concentration on Starch and Starch Synthesis Enzymes of Yangmai 16 Under Fully Open-Air Field Conditions

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