Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (1): 76-86.DOI: 10.1016/S2095-3119(14)60977-1

• 论文 • 上一篇    下一篇

Programmed cell death, antioxidant response and oxidative stress in wheat flag leaves induced by chemical hybridization agent SQ-1

 WANG Shu-ping, ZHANG Gai-sheng, SONG Qi-lu, ZHANG Ying-xin, LI Ying, GUO Jia-lin, CHEN Zheng, NIU Na, MA Shou-cai, WANG Jun-wei   

  1. 1、College of Agronomy, Northwest A&F University/National Yangling Agricultural Biotechnology & Breeding Center/Yangling Branch
    of State Wheat Improvement Centre/Wheat Breeding Engineering Research Center, Ministry of Education/Key Laboratory of Crop
    Heterosis of Shaanxi Province, Yangling 712100, P.R.China
    2、Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, P.R.China
  • 收稿日期:2014-10-15 出版日期:2016-01-08 发布日期:2016-01-11
  • 通讯作者: ZHANG Gai-sheng, Tel/Fax: +86-29-87092085,E-mail: zhanggsh58@aliyun.com
  • 作者简介:WANG Shu-ping, E-mail: wangshuping2003@126.com;
  • 基金资助:

    This research was supported by the National High Technology Research and Development Program of China (2011AA10A106), the National Natural Science Foundation of China (31171611, 31371697), the Technological Innovation and Over Planning Projects of Shaanxi Province, China (2014KTZB02-01-02, 2011KTZB02-01-01), and the Projects Opening Up New Function of Precision Instrument of Northwest A&F University, China (dysb130210).

Programmed cell death, antioxidant response and oxidative stress in wheat flag leaves induced by chemical hybridization agent SQ-1

 WANG Shu-ping, ZHANG Gai-sheng, SONG Qi-lu, ZHANG Ying-xin, LI Ying, GUO Jia-lin, CHEN Zheng, NIU Na, MA Shou-cai, WANG Jun-wei   

  1. 1、College of Agronomy, Northwest A&F University/National Yangling Agricultural Biotechnology & Breeding Center/Yangling Branch
    of State Wheat Improvement Centre/Wheat Breeding Engineering Research Center, Ministry of Education/Key Laboratory of Crop
    Heterosis of Shaanxi Province, Yangling 712100, P.R.China
    2、Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, P.R.China
  • Received:2014-10-15 Online:2016-01-08 Published:2016-01-11
  • Contact: ZHANG Gai-sheng, Tel/Fax: +86-29-87092085,E-mail: zhanggsh58@aliyun.com
  • About author:WANG Shu-ping, E-mail: wangshuping2003@126.com;
  • Supported by:

    This research was supported by the National High Technology Research and Development Program of China (2011AA10A106), the National Natural Science Foundation of China (31171611, 31371697), the Technological Innovation and Over Planning Projects of Shaanxi Province, China (2014KTZB02-01-02, 2011KTZB02-01-01), and the Projects Opening Up New Function of Precision Instrument of Northwest A&F University, China (dysb130210).

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摘要： Male sterility induced by a chemical hybridization agent (CHA) is an important tool for utilizing crop heterosis. Leaves, especially the flag leaves, as CHA initial recipients play a decisive role in inducing male sterility. To investigate effects of different treatment times of CHA-SQ-1 used, morphological, biochemical and physiological responses of wheat flag leaves were detected in this study. CHA induced programmed cell death (PCD) as shown in terminal deoxynucleotidyl transferase-mediated dUTP nick end-labelling (TUNEL) and DNA laddering analysis. In the early phase, CHA-SQ-1 triggered organelle changes and PCD in wheat leaves accompanied by excess production of reactive oxygen species (O2 -. and H2O2) and down-regulation of the activities of superoxide dismutase (SOD), catalase (CAT) and guaiacol peroxidase (POD). Meanwhile, leaf cell DNAs showed ladder-like patterns on agarose gel, indicating that CHA-SQ-1 led to the activation of the responsible endonuclease. The oxidative stress assays showed that lipid peroxidation was strongly activated and photosynthesis was obviously inhibited in SQ-1-induced leaves. However, CHA contents in wheat leaves gradually reduced along with the time CHA-SQ-1 applied. Young flags returned to an oxidative/antioxidative balance and ultimately developed into mature green leaves. These results provide explanation of the relations between PCD and anther abortion and practical application of CHA for hybrid breeding.

关键词: wheat flag leaf , chemical hybridization agent SQ-1 , programmed cell death , antioxidant response , oxidative stress

Abstract: Male sterility induced by a chemical hybridization agent (CHA) is an important tool for utilizing crop heterosis. Leaves, especially the flag leaves, as CHA initial recipients play a decisive role in inducing male sterility. To investigate effects of different treatment times of CHA-SQ-1 used, morphological, biochemical and physiological responses of wheat flag leaves were detected in this study. CHA induced programmed cell death (PCD) as shown in terminal deoxynucleotidyl transferase-mediated dUTP nick end-labelling (TUNEL) and DNA laddering analysis. In the early phase, CHA-SQ-1 triggered organelle changes and PCD in wheat leaves accompanied by excess production of reactive oxygen species (O2 -. and H2O2) and down-regulation of the activities of superoxide dismutase (SOD), catalase (CAT) and guaiacol peroxidase (POD). Meanwhile, leaf cell DNAs showed ladder-like patterns on agarose gel, indicating that CHA-SQ-1 led to the activation of the responsible endonuclease. The oxidative stress assays showed that lipid peroxidation was strongly activated and photosynthesis was obviously inhibited in SQ-1-induced leaves. However, CHA contents in wheat leaves gradually reduced along with the time CHA-SQ-1 applied. Young flags returned to an oxidative/antioxidative balance and ultimately developed into mature green leaves. These results provide explanation of the relations between PCD and anther abortion and practical application of CHA for hybrid breeding.

Key words: wheat flag leaf , chemical hybridization agent SQ-1 , programmed cell death , antioxidant response , oxidative stress