Homopteran Vector Biomarkers for Efficient Circulative Plant Virus Transmission are Conserved in Multiple Aphid Species and the Whitefly Bemisia tabaci

doi:10.1016/S1671-2927(00)8542

Journal of Integrative Agriculture ›› 2012, Vol. 11 ›› Issue (2): 249-262.DOI: 10.1016/S1671-2927(00)8542

Homopteran Vector Biomarkers for Efficient Circulative Plant Virus Transmission are Conserved in Multiple Aphid Species and the Whitefly Bemisia tabaci

Michelle Cilia, Michael Bereman, Tara Fish, Michael J MacCoss , Stewart Gray

1.USDA-ARS, Robert W. Holley Center for Agriculture and Health, Tower Road, Ithaca, New York 14853, USA
2.Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, New York 14853, USA
3.Department of Genome Sciences, University of Washington, Seattle, Washington 98195-5065, USA

收稿日期:2011-03-15 出版日期:2012-02-01 发布日期:2012-02-11
通讯作者: Correspondence Stewart Gray, Tel: +1-607-2554596 (lab), +1-607-2557844 (office), Fax: +1-607-2552739, E-mail:smg3@cornell.edu
作者简介:Michelle Cilia, E-mail:.mlc68@cornell.edu
基金资助:
This work was supported by NSF BREAD IOS:1109989, USDA-NRI 2007-04567, NSF DBI-0606596, and USDA-ARS 764 CRIS projects 1907-101- 16, 1907-21000-024/25-00D and NIH/NCRR funded Yeast Resource Center P41 RR01182.

Homopteran Vector Biomarkers for Efficient Circulative Plant Virus Transmission are Conserved in Multiple Aphid Species and the Whitefly Bemisia tabaci

Michelle Cilia, Michael Bereman, Tara Fish, Michael J MacCoss , Stewart Gray

1.USDA-ARS, Robert W. Holley Center for Agriculture and Health, Tower Road, Ithaca, New York 14853, USA
2.Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, New York 14853, USA
3.Department of Genome Sciences, University of Washington, Seattle, Washington 98195-5065, USA

Received:2011-03-15 Online:2012-02-01 Published:2012-02-11
Contact: Correspondence Stewart Gray, Tel: +1-607-2554596 (lab), +1-607-2557844 (office), Fax: +1-607-2552739, E-mail:smg3@cornell.edu
About author:Michelle Cilia, E-mail:.mlc68@cornell.edu
Supported by:
This work was supported by NSF BREAD IOS:1109989, USDA-NRI 2007-04567, NSF DBI-0606596, and USDA-ARS 764 CRIS projects 1907-101- 16, 1907-21000-024/25-00D and NIH/NCRR funded Yeast Resource Center P41 RR01182.

摘要/Abstract

摘要： Plant viruses in the families Luteoviridae and Geminiviridae are phloem restricted and are transmitted in a persistent, circulative manner by homopteran insects. Using fluorescence 2-D difference gel electrophoresis to compare the proteomes of F2 genotypes of Schizaphis graminum segregating for virus transmission ability, we recently discovered a panel of protein biomarkers that predict vector competency. Here we used aphid and whitefly nucleotide and expressed sequence tag database mining to test whether these biomarkers are conserved in other homopteran insects. S. graminum gene homologs that shared a high degree of predicted amino acid identity were discovered in two other aphid species and in the whitefly Bemisia tabaci. Selected reaction monitoring mass spectrometry was used to validate the expression of these biomarkers proteins in multiple aphid vector species. The conservation of these proteins in multiple insect taxa that transmit plant viruses along the circulative transmission pathway creates the opportunity to use these biomarkers to rapidly identify insect populations that are the most efficient vectors and allow them to be targeted for control prior to the spread of virus within a crop.

关键词: aphid, whitefly, Schizaphis graminum, Bemisia tabaci, Acyrthosiphon pisum, Toxoptera citricida, Myzus persicae, proteomics, biomarker, circulative transmission, begomovirus, luteovirus, geminivirus, leafhopper, selected reaction monitoring, SRM, mass spectrometry, targeted proteomics, Skyline

Abstract: Plant viruses in the families Luteoviridae and Geminiviridae are phloem restricted and are transmitted in a persistent, circulative manner by homopteran insects. Using fluorescence 2-D difference gel electrophoresis to compare the proteomes of F2 genotypes of Schizaphis graminum segregating for virus transmission ability, we recently discovered a panel of protein biomarkers that predict vector competency. Here we used aphid and whitefly nucleotide and expressed sequence tag database mining to test whether these biomarkers are conserved in other homopteran insects. S. graminum gene homologs that shared a high degree of predicted amino acid identity were discovered in two other aphid species and in the whitefly Bemisia tabaci. Selected reaction monitoring mass spectrometry was used to validate the expression of these biomarkers proteins in multiple aphid vector species. The conservation of these proteins in multiple insect taxa that transmit plant viruses along the circulative transmission pathway creates the opportunity to use these biomarkers to rapidly identify insect populations that are the most efficient vectors and allow them to be targeted for control prior to the spread of virus within a crop.

Key words: aphid, whitefly, Schizaphis graminum, Bemisia tabaci, Acyrthosiphon pisum, Toxoptera citricida, Myzus persicae, proteomics, biomarker, circulative transmission, begomovirus, luteovirus, geminivirus, leafhopper, selected reaction monitoring, SRM, mass spectrometry, targeted proteomics, Skyline

Michelle Cilia, Michael Bereman, Tara Fish, Michael J MacCoss , Stewart Gray. Homopteran Vector Biomarkers for Efficient Circulative Plant Virus Transmission are Conserved in Multiple Aphid Species and the Whitefly Bemisia tabaci[J]. Journal of Integrative Agriculture, 2012, 11(2): 249-262.

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Homopteran Vector Biomarkers for Efficient Circulative Plant Virus Transmission are Conserved in Multiple Aphid Species and the Whitefly Bemisia tabaci

Homopteran Vector Biomarkers for Efficient Circulative Plant Virus Transmission are Conserved in Multiple Aphid Species and the Whitefly Bemisia tabaci

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