Genetic and Association Mapping Study of English Grain Aphid Resistance and Tolerance in Bread Wheat Germplasm

doi:10.1016/S2095-3119(13)60356-1

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (1): 40-53.DOI: 10.1016/S2095-3119(13)60356-1

Genetic and Association Mapping Study of English Grain Aphid Resistance and Tolerance in Bread Wheat Germplasm

LI Feng-qi, PENG Jun-hua

1.Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, P.R.China
2.University of Chinese Academy of Sciences, Beijing 100049, P.R.China
3.Department of Soil and Crop Sciences, Colorado State University, CO 80526-1170, USA

收稿日期:2012-12-28 出版日期:2014-01-01 发布日期:2014-01-04
通讯作者: PENG Jun-hua, Tel/Fax: +86-27-87510525, E-mail: jpeng@wbgcas, jpeng@lamar.colostate.edu
作者简介:LI Feng-qi, E-mail: pandit@163.com
基金资助:
This work was supported by the National Basic Research Program of China (2009CB118300).

Genetic and Association Mapping Study of English Grain Aphid Resistance and Tolerance in Bread Wheat Germplasm

LI Feng-qi, PENG Jun-hua

1.Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, P.R.China
2.University of Chinese Academy of Sciences, Beijing 100049, P.R.China
3.Department of Soil and Crop Sciences, Colorado State University, CO 80526-1170, USA

Received:2012-12-28 Online:2014-01-01 Published:2014-01-04
Contact: PENG Jun-hua, Tel/Fax: +86-27-87510525, E-mail: jpeng@wbgcas, jpeng@lamar.colostate.edu
About author:LI Feng-qi, E-mail: pandit@163.com
Supported by:
This work was supported by the National Basic Research Program of China (2009CB118300).

摘要/Abstract

摘要： English grain aphid (EGA) is a destructive insect pest of wheat. To identify the loci associated with EGA resistance and tolerance, 70 bread wheat accessions mainly from central Asia were evaluated for EGA resistance and tolerance traits at two locations, and genotyped with 51 SSR markers. Totally, three accessions showed high or moderate levels resistance and 17 genotypes displayed highly or moderately tolerate to EGA. Genetic diversity of these lines was investigated also. After 97 SSR loci which evenly covered all wheat chromosomes were scanned for association, four SSR loci were significantly associated with EGA resistance and four with EGA tolerance. After association analysis was conducted with dynamic aphid densities, we found four loci Xgwm192b, Xgwm391, Xbarc98, and Xgwm613b were detected continuously at different growing stages of wheat. In addition, the loci of EGA resistance/tolerance and Russian wheat aphid resistance were compared. The results generated in this study would be helpful for utilization of the EGA resistance/tolerance germplasm, and for development of mapping populations in EGA resistance breeding programs.

关键词: English grain aphid , resistance , tolerance , association mapping , Triticum aestivum

Abstract: English grain aphid (EGA) is a destructive insect pest of wheat. To identify the loci associated with EGA resistance and tolerance, 70 bread wheat accessions mainly from central Asia were evaluated for EGA resistance and tolerance traits at two locations, and genotyped with 51 SSR markers. Totally, three accessions showed high or moderate levels resistance and 17 genotypes displayed highly or moderately tolerate to EGA. Genetic diversity of these lines was investigated also. After 97 SSR loci which evenly covered all wheat chromosomes were scanned for association, four SSR loci were significantly associated with EGA resistance and four with EGA tolerance. After association analysis was conducted with dynamic aphid densities, we found four loci Xgwm192b, Xgwm391, Xbarc98, and Xgwm613b were detected continuously at different growing stages of wheat. In addition, the loci of EGA resistance/tolerance and Russian wheat aphid resistance were compared. The results generated in this study would be helpful for utilization of the EGA resistance/tolerance germplasm, and for development of mapping populations in EGA resistance breeding programs.

Key words: English grain aphid , resistance , tolerance , association mapping , Triticum aestivum

LI Feng-qi, PENG Jun-hua. Genetic and Association Mapping Study of English Grain Aphid Resistance and Tolerance in Bread Wheat Germplasm[J]. Journal of Integrative Agriculture, 2014, 13(1): 40-53.

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Genetic and Association Mapping Study of English Grain Aphid Resistance and Tolerance in Bread Wheat Germplasm

Genetic and Association Mapping Study of English Grain Aphid Resistance and Tolerance in Bread Wheat Germplasm

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