Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (9): 1786-1795.DOI: 10.1016/S2095-3119(14)60931-X

• 论文 • 上一篇    下一篇

Cryptic species composition and genetic diversity within Bemisia tabaci complex in soybean in India revealed by mtCOI DNA sequence

 Prasanna H C, Kanakala S, Archana K, Jyothsna P, Varma R K, Malathi V G   

  1. 1、Division of Vegetable Improvement, Indian Institute of Vegetable Research, Varanasi, Uttar Pradesh 221305, India
    2、Advanced Centre for Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi 110012, India
    3、Department of Plant Pathology, Jawaharlal Nehru Krishi Vishwavidyalaya, Jabalpur 482004, India
  • 收稿日期:2014-08-19 出版日期:2015-09-03 发布日期:2015-09-05
  • 通讯作者: Malathi V G, Tel: +91-8110996093, Fax: +91-11-25843123, E-mail: vgmalathi@rediffmail.com
  • 作者简介:* These authors contributed equally to this work.
  • 基金资助:

    We are thankful to Department of Biotechnology, Government of India for liberal funding (Functional Genomics of Yellow Mosaic Viruses of Soybean and Development of Transgenic Resistance in Soybean: BT/PR9631/AGR/02/468/2007).

Cryptic species composition and genetic diversity within Bemisia tabaci complex in soybean in India revealed by mtCOI DNA sequence

 Prasanna H C, Kanakala S, Archana K, Jyothsna P, Varma R K, Malathi V G   

  1. 1、Division of Vegetable Improvement, Indian Institute of Vegetable Research, Varanasi, Uttar Pradesh 221305, India
    2、Advanced Centre for Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi 110012, India
    3、Department of Plant Pathology, Jawaharlal Nehru Krishi Vishwavidyalaya, Jabalpur 482004, India
  • Received:2014-08-19 Online:2015-09-03 Published:2015-09-05
  • Contact: Malathi V G, Tel: +91-8110996093, Fax: +91-11-25843123, E-mail: vgmalathi@rediffmail.com
  • About author:* These authors contributed equally to this work.
  • Supported by:

    We are thankful to Department of Biotechnology, Government of India for liberal funding (Functional Genomics of Yellow Mosaic Viruses of Soybean and Development of Transgenic Resistance in Soybean: BT/PR9631/AGR/02/468/2007).

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摘要： Bemisia tabaci is a cryptic species complex, causing significant loss on many agriculturally important crops worldwide. Knowledge on species composition and diversity within B. tabaci complex is critical for evolving sustainable pest management strategies. Here we investigate the whitefly species complex in soybean in major soybean growing states of India. The mitochondrial cytochrome oxidase gene subunit-1 (mtCOI) based phylogenetic relationships established using Bayesian methods indicated the existence of three cryptic species namely Asia I, Asia II 1, and Asia II 7. All the haplotypes detected in the study could be assigned to these three cryptic species following the species demarcation criteria of 3.5% divergence threshold. Of these, Asia II 1 was found to be predominant with wide spread distribution across the surveyed regions from cool temperate zones to hot and humid tropical plains. On the contrary, cryptic species Asia II 7 showed localized distribution. The Asia II 1 exhibited the highest haplotype diversity and Asia I showed high level of nucleotide diversity. There was a significantly high genetic differentiation among these three cryptic species. The MEAM 1, a dreadful invasive species was not detected in the specimens tested in the current study. The diversity and distribution of three cryptic species is discussed in the light of current knowledge on distribution of whitefly species in India and yellow mosaic disease observed during sampling survey.

关键词: whitefly , mitochondrial cytochrome oxidase gene subunit-1 , Asia I , Asia II 1 , Asia II 7 , begomovirus and yellow mosaic disease

Abstract: Bemisia tabaci is a cryptic species complex, causing significant loss on many agriculturally important crops worldwide. Knowledge on species composition and diversity within B. tabaci complex is critical for evolving sustainable pest management strategies. Here we investigate the whitefly species complex in soybean in major soybean growing states of India. The mitochondrial cytochrome oxidase gene subunit-1 (mtCOI) based phylogenetic relationships established using Bayesian methods indicated the existence of three cryptic species namely Asia I, Asia II 1, and Asia II 7. All the haplotypes detected in the study could be assigned to these three cryptic species following the species demarcation criteria of 3.5% divergence threshold. Of these, Asia II 1 was found to be predominant with wide spread distribution across the surveyed regions from cool temperate zones to hot and humid tropical plains. On the contrary, cryptic species Asia II 7 showed localized distribution. The Asia II 1 exhibited the highest haplotype diversity and Asia I showed high level of nucleotide diversity. There was a significantly high genetic differentiation among these three cryptic species. The MEAM 1, a dreadful invasive species was not detected in the specimens tested in the current study. The diversity and distribution of three cryptic species is discussed in the light of current knowledge on distribution of whitefly species in India and yellow mosaic disease observed during sampling survey.

Key words: whitefly , mitochondrial cytochrome oxidase gene subunit-1 , Asia I , Asia II 1 , Asia II 7 , begomovirus and yellow mosaic disease