Responses of Detoxifying, Antioxidant and Digestive Enzyme Activities to Host Shift of Bemisia tabaci (Hemiptera: Aleyrodidae)

doi:10.1016/S2095-3119(13)60228-2

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (2): 296-304.DOI: 10.1016/S2095-3119(13)60228-2

Responses of Detoxifying, Antioxidant and Digestive Enzyme Activities to Host Shift of Bemisia tabaci (Hemiptera: Aleyrodidae)

DENG Pan, CHEN Long-jia, ZHANG Zong-lei, LIN Ke-jian , MA Wei-hua

1.Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, Huazhong Agricultural University, Wuhan 430070,P.R.China
2.Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China

收稿日期:2011-11-22 出版日期:2013-02-01 发布日期:2013-02-06
通讯作者: Correspondence MA Wei-hua, Tel: +86-27-87287207, Fax: +86-27-87281938, E-mail: weihuama@mail.hzau.edu.cn; LIN Ke-jian, Tel: +86-10-62815909, Fax: +86-10- 62896114, E-mail: kjlin@ippcaas.cn
基金资助:
This study was supported by the National Natural Science Foundation of China (30800131) and the Fundamental Research Funds for the Central Universities, China (2012ZYTS043).

Responses of Detoxifying, Antioxidant and Digestive Enzyme Activities to Host Shift of Bemisia tabaci (Hemiptera: Aleyrodidae)

DENG Pan, CHEN Long-jia, ZHANG Zong-lei, LIN Ke-jian , MA Wei-hua

1.Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, Huazhong Agricultural University, Wuhan 430070,P.R.China
2.Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China

Received:2011-11-22 Online:2013-02-01 Published:2013-02-06
Contact: Correspondence MA Wei-hua, Tel: +86-27-87287207, Fax: +86-27-87281938, E-mail: weihuama@mail.hzau.edu.cn; LIN Ke-jian, Tel: +86-10-62815909, Fax: +86-10- 62896114, E-mail: kjlin@ippcaas.cn
Supported by:
This study was supported by the National Natural Science Foundation of China (30800131) and the Fundamental Research Funds for the Central Universities, China (2012ZYTS043).

摘要/Abstract

摘要： The polyphagous B-biotype of Bemisia tabaci (Gennadius) has demonstrated a high capacity to adapt to numerous hosts from diverse plant families. To illustrate induced responses by the host plant, biochemical research on eight plant-insect interaction correlative enzymes, representing detoxifying, antioxidant and digestive pathways, were investigated. Transferring whitefly adults to Zhongmian 23 from the pre-adapted host Zhongmian 41 induced activities of carboxylesterase (by 1.80-fold), glutathione S-transferase (by 3.79-fold), proteinase (by 1.62-fold) and amylase (by 2.41-fold) activities, but decreased polyphenol oxidase (by 1.89-fold) and peroxidase (by 1.88-fold). However, transferring whitefly adults to the favorite host abutilon from Zhongmian 41 was associated with increased activities of cytochrome P450 monooxygenase (by 1.61-fold) and amylase (by 1.42-fold), and decreased activities of polyphenol oxidase (by 2.96-fold) and peroxidase (by 2.07-fold). Our results, together with previous studies, proved that multiple pathways are involved in coping with host shifts by polyphagous herbivores, and the taxonomic status and preference of the host transferred would affect which pathway would be induced. These results would represent a key challenge in developing long-term sustainable insect control strategies.

关键词: Bemisia tabaci , biotype B , host shift , antioxidant enzyme , detoxifying enzyme , digestive enzyme

Abstract: The polyphagous B-biotype of Bemisia tabaci (Gennadius) has demonstrated a high capacity to adapt to numerous hosts from diverse plant families. To illustrate induced responses by the host plant, biochemical research on eight plant-insect interaction correlative enzymes, representing detoxifying, antioxidant and digestive pathways, were investigated. Transferring whitefly adults to Zhongmian 23 from the pre-adapted host Zhongmian 41 induced activities of carboxylesterase (by 1.80-fold), glutathione S-transferase (by 3.79-fold), proteinase (by 1.62-fold) and amylase (by 2.41-fold) activities, but decreased polyphenol oxidase (by 1.89-fold) and peroxidase (by 1.88-fold). However, transferring whitefly adults to the favorite host abutilon from Zhongmian 41 was associated with increased activities of cytochrome P450 monooxygenase (by 1.61-fold) and amylase (by 1.42-fold), and decreased activities of polyphenol oxidase (by 2.96-fold) and peroxidase (by 2.07-fold). Our results, together with previous studies, proved that multiple pathways are involved in coping with host shifts by polyphagous herbivores, and the taxonomic status and preference of the host transferred would affect which pathway would be induced. These results would represent a key challenge in developing long-term sustainable insect control strategies.

Key words: Bemisia tabaci , biotype B , host shift , antioxidant enzyme , detoxifying enzyme , digestive enzyme

DENG Pan, CHEN Long-jia, ZHANG Zong-lei, LIN Ke-jian , MA Wei-hua. Responses of Detoxifying, Antioxidant and Digestive Enzyme Activities to Host Shift of Bemisia tabaci (Hemiptera: Aleyrodidae)[J]. Journal of Integrative Agriculture, 2013, 12(2): 296-304.

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Responses of Detoxifying, Antioxidant and Digestive Enzyme Activities to Host Shift of Bemisia tabaci (Hemiptera: Aleyrodidae)

Responses of Detoxifying, Antioxidant and Digestive Enzyme Activities to Host Shift of Bemisia tabaci (Hemiptera: Aleyrodidae)

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