菜用大豆外观品质性状的全基因组关联分析及KASP标记开发

doi:10.1016/j.jia.2024.09.005

Journal of Integrative Agriculture ›› 2026, Vol. 25 ›› Issue (6): 2341-2352.DOI: 10.1016/j.jia.2024.09.005

菜用大豆外观品质性状的全基因组关联分析及KASP标记开发

收稿日期:2024-04-27 修回日期:2024-09-12 接受日期:2024-07-04 出版日期:2026-06-20 发布日期:2026-05-06

Genome-wide association study of appearance quality traits and development of KASP makers in vegetable soybean

Shuo Yang^1*, Qianru Jia^{2, 4*}, Qiong Wang^{2, 4}, Junyan Wang^{2, 4}, Jiahao Li³, Shengyan Hu^{2, 4}, Wei Zhang^{2, 4}, Hongmei Zhang^{2, 4}, Ya Guo³, Xin Chen⁴, Yuelin Zhu^1#, Huatao Chen^{2, 4#}

¹College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
²Zhongshan Biological Breeding Laboratory (ZSBBL), Nanjing 210014, China
³The School of IoT (Internet of Things), Jiangnan University, Wuxi 214122, China
⁴Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

Received:2024-04-27 Revised:2024-09-12 Accepted:2024-07-04 Online:2026-06-20 Published:2026-05-06
About author:#Correspondence Yuelin Zhu, E-mail: ylzhu@njau.edu.cn; Huatao Chen, E-mail: cht@jaas.ac.cn * These authors contributed equally to this study.
Supported by:
This study was supported by the Zhongshan Biological Breeding Laboratory, China (ZSBBL-KY2023-03), the Jiangsu Agricultural Science and Technology Innovation Fund, China (CX(22)5002) and a project funded by the China Postdoctoral Science Foundation (2023M731401).

摘要/Abstract

摘要：

菜用大豆（Glycine max（L.）Merr.），通常称为毛豆，是一种在豆荚结荚期（R6）收获的豆类蔬菜，在中国农业中具有重要地位。菜用大豆的外观品质对消费者的偏好和市场适销性至关重要，这些品质性状包括豆荚的长度、宽度和颜色等因素。本研究在南京、淮安和南通三地种植了264种菜用大豆，并使用PlantPhenoM（一种豆荚表型鉴定和分析系统）对豆荚性状进行了评估。结果显示，菜用大豆外观品质性状的变异范围为8.64%至30.00%。通过表型数据和全基因组关联研究（GWAS），我们鉴定出了525个与不同地区外观质量性状显著相关的SNP位点。此外，还筛选出了与目标性状相关的候选基因Glyma.04G004700、Glyma.15G051600、Glyma.18G25700、Glyma.18G225900和Glyma.18G272300，并成功开发了KASP标记。本研究为培育优良的菜用大豆品种提供了宝贵的见解，并为菜用大豆外观品质性状候选基因及分子标记相关研究奠定了基础。

关键词:

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Abstract:

Vegetable soybean ((Glycine max (L.) Merr.), commonly referred to as edamame, holds significant agricultural importance in China as a legume vegetable harvested at the pod-filling stage (R6). The visual appeal of vegetable soybeans is crucial for consumer preference and marketability, and it depends on factors such as pod length, pod width, and pod color. This study cultivated 264 vegetable soybeans in Nanjing, Huai’an, and Nantong, Jiangsu Province, China to assess pod traits using PlantPhenoM, a system for pod phenotypic identification and analysis. The results revealed a variability range of 8.64 to 30.00% in appearance quality traits among the vegetable soybeans. Leveraging phenotypic data and employing a genome-wide association study (GWAS) identified 525 SNPs significantly linked to the appearance quality traits in different regions. In addition, five candidate genes (Glyma.04G004700, Glyma.15G051600, Glyma.18G225700, Glyma.18G225900, and Glyma.18G272300) associated with target traits were identified, and KASP markers for S04_372771 (pod length), S18_51477324 (pod width), and S18_55553200 (pod color) were developed. This study offers valuable insights for breeding superior vegetable soybean varieties and lays the groundwork for exploring candidate genes and molecular markers related to appearance and quality traits in vegetable soybeans.

Key words: appearance quality , genome-wide association study , KASP marker , vegetable soybean

Shuo Yang, Qianru Jia, Qiong Wang, Junyan Wang, Jiahao Li, Shengyan Hu, Wei Zhang, Hongmei Zhang, Ya Guo, Xin Chen, Yuelin Zhu, Huatao Chen. 菜用大豆外观品质性状的全基因组关联分析及KASP标记开发[J]. Journal of Integrative Agriculture, 2026, 25(6): 2341-2352.

Shuo Yang, Qianru Jia, Qiong Wang, Junyan Wang, Jiahao Li, Shengyan Hu, Wei Zhang, Hongmei Zhang, Ya Guo, Xin Chen, Yuelin Zhu, Huatao Chen. Genome-wide association study of appearance quality traits and development of KASP makers in vegetable soybean[J]. Journal of Integrative Agriculture, 2026, 25(6): 2341-2352.

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菜用大豆外观品质性状的全基因组关联分析及KASP标记开发

Genome-wide association study of appearance quality traits and development of KASP makers in vegetable soybean

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