Journal of Integrative Agriculture ›› 2024, Vol. 23 ›› Issue (1): 59-76.DOI: 10.1016/j.jia.2023.07.010
所属专题： 水稻遗传育种合辑Rice Genetics · Breeding · Germplasm Resources
SUPER WOMAN 2 (SPW2) 通过抑制花同源异型基因OsMADS3、OsMADS58、OsMADS13和DROOPING LEAF的表达维持小穗的器官特征
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收稿日期:2023-03-03接受日期:2023-06-20出版日期:2024-01-20发布日期:2024-01-05
SUPER WOMAN 2 (SPW2) maintains organ identity in spikelets by inhibiting the expression of floral homeotic genes OsMADS3, OsMADS58, OsMADS13, and DROOPING LEAF
Hui Zhuang*, Jinsong
Lan*, Qiuni Yang*, Xiaoyu Zhao, Yuhuan Li, Jingya Zhi,
Yalin Shen, Guanghua He, Yunfeng Li#
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Rice Research Institute, Key Laboratory of Application and Safety Control of Genetically Modified Crops, Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China
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Received:2023-03-03Accepted:2023-06-20Online:2024-01-20Published:2024-01-05 -
About author:Hui Zhuang, E-mail: amberzzh@163.com;#Correspondence Yunfeng Li, Tel: +86-23-68250158, E-mail: liyf1980@swu.edu.cn -
Supported by:This work was supported by the Chongqing Modern Agricultural Industry Technology System, China (CQMAITS202301), the National Natural Science Foundation of China (32100287 and 31971919), the Natural Science Foundation of Chongqing, China (cstc2020jcyj-jqX0020 and cstc2021ycjh-bgzxm0066), and the China Postdoctoral Science Foundation Funded Project (2020M683219), and the Fundamental Research Funds for the Central Universities, China (SWU-XDJH202315).
摘要：
水稻的花器官特征主要由A、B、C、E四类基因决定，它们大多编码MADS-box转录因子。然而，在花发育过程中，对于这些基因的表达如何被调控的研究很少。本研究报道了一个名为SUPER WOMAN 2（SPW2）的基因，该基因在水稻的小穗/小花发育过程中通过调控雌蕊特征基因OsMADS3、OsMADS13、OsMADS58和DL的表达发挥重要作用。SPW2突变导致小穗内的护颖、外稃、内稃、浆片和雄蕊中出现异位的柱头/子房状组织。通过图位克隆，我们揭示了SPW2编码一个植物特有的类EMF1蛋白，该蛋白是PRC2复合物的重要组成部分，并介导H3K27me3修饰。表达分析显示，SPW2突变导致OsMADS3、OsMADS13、OsMADS58和DL在小穗的非雌蕊器官中异位表达。此外，ChIP-qPCR结果显示这些基因在染色质上的H3K27me3修饰水平显著降低。因此，我们的研究结果表明SPW2通过参与H3K27me3介导的雌蕊特征基因表观遗传沉默，进而调控它们在水稻小穗的非雌蕊器官中的表达。这项研究拓宽了我们对于SPW2通过表观遗传调控花器官特征基因的分子机制的认识。
引用本文
. SUPER WOMAN 2 (SPW2) 通过抑制花同源异型基因OsMADS3、OsMADS58、OsMADS13和DROOPING LEAF的表达维持小穗的器官特征 [J]. Journal of Integrative Agriculture, 2024, 23(1): 59-76.
ZHUANG Hui, LAN Jin-song, YANG Qiu-ni, ZHAO Xiao-yu, LI Yu-huan, ZHI Jing-ya, SHEN Ya-lin, HE Guang-hua, LI Yun-feng. SUPER WOMAN 2 (SPW2) maintains organ identity in spikelets by inhibiting the expression of floral homeotic genes OsMADS3, OsMADS58, OsMADS13, and DROOPING LEAF[J]. Journal of Integrative Agriculture, 2024, 23(1): 59-76.
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